Improving the phosphoproteome coverage for limited sample amounts using TiO2-SIMAC-HILIC (TiSH) phosphopeptide enrichment and fractionation

Methods in Molecular Biology

Volumn 1355, Issue , 2016, Pages 161-177

  Engholm Keller, Kasper ^a   Larsen, Martin R ^a  

^a NONE

Author keywords

Capillary HPLC; Enrichment; Fractionation; HILIC; Phosphopeptide; Phosphoproteomics; Protein phosphorylation; TiO2; TiSH

Indexed keywords

PHOSPHOPEPTIDE; TITANIUM DIOXIDE; PHOSPHOPROTEIN; TITANIUM;

CYTOLYSIS; ELUTION; FRACTIONATION; HYDROPHILIC INTERACTION CHROMATOGRAPHY; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; PHOSPHOPROTEOMICS; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN PHOSPHORYLATION; PROTEOMICS; TANDEM MASS SPECTROMETRY; AFFINITY CHROMATOGRAPHY; ANIMAL; CELL LINE; CHEMISTRY; HUMAN; METABOLISM; PEPTIDE MAPPING; PHOSPHORYLATION; PROCEDURES; PROTEIN PROCESSING; WORKFLOW;

ANIMALS; CELL LINE; CHROMATOGRAPHY, AFFINITY; HUMANS; PEPTIDE MAPPING; PHOSPHOPROTEINS; PHOSPHORYLATION; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEOMICS; TANDEM MASS SPECTROMETRY; TITANIUM; WORKFLOW;

EID: 84947997488     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-3049-4_11     Document Type: Chapter

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