Efficient CRISPR/Cas9-mediated multiplex genome editing in CHO cells via high-level sgRNA-Cas9 complex

Biotechnology and Bioprocess Engineering

Volumn 20, Issue 5, 2015, Pages 825-833

  Shin, Jongoh ^a   Lee, Namil ^a   Song, Yoseb ^a   Park, Jinhyung ^a   Kang, Taek Jin ^b   Kim, Sun Chang ^a,b   Lee, Gyun Min ^a   Cho, Byung Kwan ^a,b  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^b Dongguk University   (South Korea)

Author keywords

Chinese hamster ovary (CHO) cells; CRISPR Cas9; genome editing; iterative transfection

Indexed keywords

CELLS; CYTOLOGY; EFFICIENCY; GENES; ITERATIVE METHODS; MOLECULAR BIOLOGY; NUCLEIC ACIDS; PROTEINS; RECOMBINANT PROTEINS; RNA;

CHINESE HAMSTER OVARY CELLS; CRISPR/CAS9; CRITICAL DETERMINANT; EFFICIENT STRATEGY; FRAME-SHIFT MUTATION; HIGH LEVEL EXPRESSION; ITERATIVE TRANSFECTION; THERAPEUTIC PROTEIN;

CELL ENGINEERING;

CAS9 NUCLEASE; CRISPR ASSOCIATED PROTEIN; GUIDE RNA; NUCLEASE; RECOMBINANT ENZYME; SINGLE GUIDE RNA; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CHO CELL LINE; COMPLEX FORMATION; CONTROLLED STUDY; CRISPR CAS SYSTEM; DNA END JOINING REPAIR; FEMALE; FRAMESHIFT MUTATION; GENE EXPRESSION; GENE LOCUS; GENE TARGETING; GENETIC ASSOCIATION; GENETIC TRANSFECTION; GENOME; GENOME EDITING; MEASUREMENT ERROR; MUTATION RATE; NONHUMAN; PROTEIN RNA BINDING; SITE DIRECTED MUTAGENESIS;

EID: 84947903179     PISSN: 12268372     EISSN: 19763816     Source Type: Journal    
DOI: 10.1007/s12257-015-0233-7     Document Type: Article

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