Time-resolved FRET strategy to screen GPCR ligand library

Methods in Molecular Biology

Volumn 1272, Issue , 2015, Pages 23-36

  Oueslati, Nadia ^a   Hounsou, Candide ^a   Belhocine, Abderazak ^a   Rodriguez, Thieric ^a   Dupuis, Elodie ^a   Zwier, Jurriaan M ^a   Trinquet, Eric ^a   Pin, Jean Philippe ^a   Durroux, Thierry ^a  

^a NONE

Author keywords

Binding experiment; Fluorescent ligand; G protein coupled receptor; Lanthanide; Self labeling enzyme; Tag lite screening; Terbium; Time resolved FRET

Indexed keywords

G PROTEIN COUPLED RECEPTOR; LIGAND; BENZYLGUANIDINE; COORDINATION COMPOUND; FLUORESCENT DYE; GUANIDINE DERIVATIVE; METHYLATED DNA PROTEIN CYSTEINE METHYLTRANSFERASE; MOLECULAR LIBRARY; PROTEIN BINDING; TERBIUM;

ANALYTIC METHOD; ARTICLE; CHEMICAL LABELING; DRUG DESIGN; EMBRYO; FLUORESCENCE RESONANCE ENERGY TRANSFER; HEK293 CELL LINE; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; MOLECULAR LIBRARY; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LABELING; AGONISTS; ANTAGONISTS AND INHIBITORS; BINDING SITE; CHEMISTRY; GENE EXPRESSION; GENETICS; HIGH THROUGHPUT SCREENING; KINETICS; METABOLISM; PHARMACOLOGY; PROCEDURES; SIGNAL TRANSDUCTION; STAINING;

BINDING SITES; COORDINATION COMPLEXES; DRUG DESIGN; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT DYES; GENE EXPRESSION; GUANIDINES; HEK293 CELLS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; KINETICS; LIGANDS; O(6)-METHYLGUANINE-DNA METHYLTRANSFERASE; PROTEIN BINDING; RECEPTORS, G-PROTEIN-COUPLED; SIGNAL TRANSDUCTION; SMALL MOLECULE LIBRARIES; STAINING AND LABELING; TERBIUM;

EID: 84947807186     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2336-6_2     Document Type: Article

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