Metabolic engineering of Escherichia coli for poly(3-hydroxybutyrate) production via threonine bypass

Microbial Cell Factories

Volumn 14, Issue 1, 2015, Pages

  Lin, Zhenquan ^a   Zhang, Yan ^a   Yuan, Qianqian ^a,b   Liu, Qiaojie ^a   Li, Yifan ^a   Wang, Zhiwen ^a   Ma, Hongwu ^b   Chen, Tao ^a   Zhao, Xueming ^a  

^a TIANJIN UNIVERSITY   (China)

^b Chinese Academy of Sciences   (China)

Author keywords

Genome scale metabolic network; Poly(3 hydroxybutyrate); Strain optimization; Threonine bypass

Indexed keywords

ACETYL COENZYME A; GLUCOSE; NICOTINAMIDE ADENINE DINUCLEOTIDE (PHOSPHATE) TRANSHYDROGENASE; POLY(3 HYDROXYBUTYRIC ACID); PYRUVATE DEHYDROGENASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; THREONINE; HYDROXYBUTYRIC ACID; POLY-BETA-HYDROXYBUTYRATE; POLYESTER;

AMINO ACID SYNTHESIS; ARTICLE; BACTERIAL GENE; BACTERIAL METABOLISM; BIOFUEL PRODUCTION; CARBON SOURCE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME MECHANISM; FED BATCH FERMENTATION; FERMENTATION TECHNIQUE; GENE OVEREXPRESSION; GENETIC ENGINEERING; GLYA GENE; NONHUMAN; PROCESS DEVELOPMENT; PROCESS OPTIMIZATION; PROTEIN DEGRADATION; SHAKE FLASH FERMENTATION; THREONINE BYPASS; ESCHERICHIA COLI; METABOLIC ENGINEERING; METABOLISM; PROCEDURES;

ESCHERICHIA COLI; HYDROXYBUTYRATES; METABOLIC ENGINEERING; POLYESTERS; THREONINE;

EID: 84947586122     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-015-0369-3     Document Type: Article

References (40)

1. Akaraonye E, Keshavarz T, Roy I. Production of polyhydroxyalkanoates: the future green materials of choice. J Chem Technol Biotechnol. 2010;85:732-43.
2. Lee SY. Deciphering bioplastic production. Nat Biotechnol. 2006;24:1227-9.
3. Chen G-Q. A microbial polyhydroxyalkanoates (PHA) based bio- and materials industry. Chem Soc Rev. 2009;38:2434.
4. Lee SY. Bacterial polyhydroxyalkanoates. Biotechnol Bioeng. 1996;49:1-14.
5. Chen G-Q, Wu Q. Jung YK. Lee SY: Pha/Phb. Compr Biotechnol; 2011. p. 217-27.
6. Leong YK, Show PL, Ooi CW, Ling TC, Lan JCW. Current trends in polyhydroxyalkanoates (PHAs) biosynthesis: insights from the recombinant Escherichia coli. J Biotechnol. 2014;180:52-65.
7. Li R, Zhang H, Qi Q. The production of polyhydroxyalkanoates in recombinant Escherichia coli. Bioresour Technol. 2007;98:2313-20.
8. Spiekermann P, Rehm BH, Kalscheuer R, Baumeister D, Steinbüchel A. A sensitive, viable-colony staining method using Nile red for direct screening of bacteria that accumulate polyhydroxyalkanoic acids and other lipid storage compounds. Arch Microbiol. 1999;171:73-80.
9. Schubert P, Steinbüchel A, Schlegel HG. Cloning of the Alcaligenes eutrophus genes for synthesis of poly-beta-hydroxybutyric acid (PHB) and synthesis of PHB in Escherichia coli. J Bacteriol. 1988;170:5837-47.
10. Lee SY. E. coli moves into the plastic age. Nat Biotechnol. 1997;15:17-8.
11. Choi JI, Lee SY. Process analysis and economic evaluation for poly(3-hydroxybutyrate) production by fermentation. Bioprocess Eng. 1997;17:335-42.
12. Khosravi-Darani K, Mokhtari ZB, Amai T, Tanaka K. Microbial production of poly(hydroxybutyrate) from C1 carbon sources. Appl Microbiol Biotechnol. 2013;97:1407-24.
13. Shi H, Nikawa J, Shimizu K. Effect of modifying metabolic network on poly-3-hydroxybutyrate biosynthesis in recombinant Escherichia coli. J Biosci Bioeng. 1999;87:666-77.
14. Jian J, Zhang SQ, Shi ZY, Wang W, Chen GQ, Wu Q. Production of polyhydroxyalkanoates by Escherichia coli mutants with defected mixed acid fermentation pathways. Appl Microbiol Biotechnol. 2010;87:2247-56.
15. Nikel PI, Pettinari MJ, Galvagno MA, Mendez BS. Poly(3-Hydroxybutyrate) synthesis by recombinant Escherichia coli arcA mutants in microaerobiosis. Appl Environ Microbiol. 2006;72:2614-20.
16. Nikel PI, Pettinari MJ, Galvagno MA, Méndez BS. Poly(3-hydroxybutyrate) synthesis from glycerol by a recombinant Escherichia coli arcA mutant in fed-batch microaerobic cultures. Appl Microbiol Biotechnol. 2008;77:1337-43.
17. Koffas M, Stephanopoulos G. Strain improvement by metabolic engineering: Lysine production as a case study for systems biology. Curr Opin Biotechnol. 2005;16(3 SPEC. ISS.):361-6.
18. Sawers G. The anaerobic degradation of l-serine and l-threonine in enterobacteria: networks of pathways and regulatory signals. Arch Microbiol. 1998;171:1-5.
19. Newman EB, Kapoor V, Potter R. Role of l threonine dehydrogenase in the catabolism of threonine and synthesis of glycine by Escherichia coli. J Bacteriol. 1976;126:1245-9.
20. Potter R, Kapoor V, Newman EB. Role of threonine dehydrogenase in Escherichia coli threonine degradation. J Bacteriol. 1977;132:385-91.
21. Marcus JP, Dekker EE. Threonine formation via the coupled activity of 2-amino-3-ketobutyrate coenzyme A lyase and threonine dehydrogenase. J Bacteriol. 1993;175:6505-11.
22. Zhang X, Newman E. Deficiency in l-serine deaminase results in abnormal growth and cell division of Escherichia coli K-12. Mol Microbiol. 2008;69:870-81.
23. Netzer R, Peters-Wendisch P, Eggeling L, Sahm H. Cometabolism of a nongrowth substrate: l-serine utilization by Corynebacterium glutamicum. Appl Environ Microbiol. 2004;70:7148-55.
24. Newman EB. Metabolism of serine and glycine in E. coli K12. I. The role of formate in the metabolism of serine-glycine auxotrophs. Can J Microbiol. 1970;16:933-40.
25. Chan TTK, Newman EB. Threonine as a carbon source for Escherichia coli. J Bacteriol. 1981;145:1150-3.
26. Ravnikar PD, Somerville RL. Genetic characterization of a highly efficient alternate pathway of serine biosynthesis in Escherichia coli. J Bacteriol. 1987;169:2611-7.
27. Wright JK, Takahashi M. Interaction of substrates and inhibitors with the homoserine dehydrogenase of kinase-inactivated aspartokinase I. Biochemistry. 1977;16:1541-8.
28. Lee J-H, Lee D-E, Lee B-U, Kim H-S. Global analyses of transcriptomes and proteomes of a parent strain and an l-threonine-overproducing mutant strain. J Bacteriol. 2003;185:5442-51.
29. Lynn SP, Gardner JF, Reznikoff WS. Attenuation regulation in the thr operon of Escherichia coli K-12: molecular cloning and transcription of the controlling region. J Bacteriol. 1982;152:363-71.
30. Lee KH, Park JH, Kim TY, Kim HU, Lee SY. Systems metabolic engineering of Escherichia coli for l-threonine production. Mol Syst Biol. 2007;3:149.
31. Mansouri A, Decter JB, Silber R. Studies on the regulation of one-carbon metabolism. II. Repression-derepression of serine hydroxymethyltransferase by methionine in Escherichia coli 113-3. J Biol Chem. 1972;247:348-52.
32. Zhang X, El-Hajj ZW, Newman E. Deficiency in l-serine deaminase interferes with one-carbon metabolism and cell wall synthesis in Escherichia coli K-12. J Bacteriol. 2010;192:5515-25.
33. Sauer U, Canonaco F, Heri S, Perrenoud A, Fischer E. The soluble and membrane-bound transhydrogenases UdhA and PntAB have divergent functions in NADPH metabolism of Escherichia coli. J Biol Chem. 2004;279:6613-9.
34. Schellenberger J, Que R, Fleming RMT, Thiele I, Orth JD, Feist AM, Zielinski DC, Bordbar A, Lewis NE, Rahmanian S, Kang J, Hyduke DR, Palsson BØ. Quantitative prediction of cellular metabolism with constraint-based models: the COBRA Toolbox v2.0. Nat Protoc. 2011;6:1290-307.
35. Li Z-J, Cai L, Wu Q, Chen G-Q. Overexpression of NAD kinase in recombinant Escherichia coli harboring the phbCAB operon improves poly(3-hydroxybutyrate) production. Appl Microbiol Biotechnol. 2009;83:939-47.
36. Lin Z, Xu Z, Li Y, Wang Z, Chen T, Zhao X. Metabolic engineering of Escherichia coli for the production of riboflavin. Microb Cell Fact. 2014;13:104.
37. Lu J, Tang J, Liu Y, Zhu X, Zhang T, Zhang X. Combinatorial modulation of galP and glk gene expression for improved alternative glucose utilization. Appl Microbiol Biotechnol. 2012;93:2455-62.
38. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods San Diego Calif. 2001;25:402-8.
39. Zhu N, Xia H, Wang Z, Zhao X, Chen T. Engineering of acetate recycling and citrate synthase to improve aerobic succinate production in Corynebacterium glutamicum. PLoS One. 2013;8:e60659. doi: 10.1371/journal.pone.0060659.
40. Kuhlman TE, Cox EC. Site-specific chromosomal integration of large synthetic constructs. Nucleic Acids Res. 2010;38:e92. doi: 10.1093/nar/gkp1193.