Engineering of Alicyclobacillus hesperidum l-Arabinose Isomerase for Improved Catalytic Activity and Reduced pH Optimum Using Random and Site-Directed Mutagenesis

Applied Biochemistry and Biotechnology

Volumn 177, Issue 7, 2015, Pages 1480-1492

  Fan, Chen ^a   Xu, Wei ^a   Zhang, Tao ^a   Zhou, Leon ^b   Jiang, Bo ^a   Mu, Wanmeng ^a  

^a JIANGNAN UNIVERSITY   (China)

^b Roquette America   (United States)

Author keywords

d tagatose; Error prone PCR; l Arabinose isomerase; Random mutagenesis; Site directed mutagenesis

Indexed keywords

BACILLI; CATALYST ACTIVITY; ISOMERIZATION; ISOMERS; MANGANESE; MUTAGENESIS;

D-TAGATOSE; ERROR PRONE PCR; L-ARABINOSE ISOMERASE; RANDOM MUTAGENESIS; SITE DIRECTED MUTAGENESIS;

POLYMERASE CHAIN REACTION;

ARABINOSE; GALACTOSE; ISOMERASE; MANGANESE; HEXOSE; L-ARABINOSE ISOMERASE; METAL; TAGATOSE;

ALICYCLOBACILLUS; ALICYCLOBACILLUS HESPERIDUM; ARTICLE; CATALYSIS; CATALYTIC EFFICIENCY; ENZYME ACTIVE SITE; ENZYME SUBSTRATE; ISOMERIZATION; MOLECULAR MODEL; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; SITE DIRECTED MUTAGENESIS; TEMPERATURE; WILD TYPE; BIOCATALYSIS; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MUTATION;

ALDOSE-KETOSE ISOMERASES; ALICYCLOBACILLUS; BIOCATALYSIS; CATALYTIC DOMAIN; HEXOSES; HYDROGEN-ION CONCENTRATION; KINETICS; METALS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; TEMPERATURE;

EID: 84947488135     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-015-1828-3     Document Type: Article

References (31)

1. Xu, Z., Li, S., Feng, X., Liang, J., & Xu, H. (2014). l-Arabinose isomerase and its use for biotechnological production of rare sugars. Applied Microbiology and Biotechnology,98, 8869–8878.
2. Oh, D. K. (2007). Tagatose: properties, applications, and biotechnological processes. Applied Microbiology and Biotechnology,76, 1–8.
3. Kim, P. (2004). Current studies on biological tagatose production using l-arabinose isomerase: a review and future perspective. Applied Microbiology and Biotechnology,65, 243–249.
4. Bertelsen, H., Jensen, B. B., & Buemann, B. (1999). d-Tagatose—a novel low-calorie bulk sweetener with prebiotic properties. World Review of Nutrition and Dietetics,85, 98–109.
5. Lo, M. C., & Lansang, M. C. (2013). Recent and emerging therapeutic medications in type 2 diabetes mellitus: incretin-based, pramlintide, colesevelam, SGLT2 inhibitors, tagatose, succinobucol. American Journal of Therapeutics,20, 638–653.
6. Kwak, J. H., Kim, M. S., Lee, J. H., Yang, Y. J., Lee, K. H., Kim, O. Y., & Lee, J. H. (2013). Beneficial effect of tagatose consumption on postprandial hyperglycemia in Koreans: a double-blind crossover designed study. Food & Function,4, 1223–1228.
7. Police, S. B., Harris, J. C., Lodder, R. A., & Cassis, L. A. (2009). Effect of diets containing sucrose vs. d-tagatose in hypercholesterolemic mice. Obesity,17, 269–275.
8. Donner, T. W., Magder, L. S., & Zarbalian, K. (2010). Dietary supplementation with d-tagatose in subjects with type 2 diabetes leads to weight loss and raises high-density lipoprotein cholesterol. Nutrition Research,30, 801–806.
9. Lu, Y., Levin, G. V., & Donner, T. W. (2008). Tagatose, a new antidiabetic and obesity control drug. Diabetes Obesity & Metabolism,10, 109–134.
10. Lu, Y., & Levin, G. V. (2002). Removal and prevention of dental plaque with d-tagatose. International Journal of Cosmetic Science,24, 225–234.
11. Friedman, M. (1996). Food browning and its prevention: an overview. Journal of Agricultural and Food Chemistry,44, 631–653.
12. Rhimi, M., & Bejar, S. (2006). Cloning, purification and biochemical characterization of metallic-ions independent and thermoactive l-arabinose isomerase from the Bacillus stearothermophilus US100 strain. Biochimica et Biophysica Acta,1760, 191–199.
13. Kim, H. J., Kim, J. H., Oh, H. J., & Oh, D. K. (2006). Characterization of a mutated Geobacillus stearothermophilusl-arabinose isomerase that increases the production rate of d-tagatose. Journal of Applied Microbiology,101, 213–221.
14. Kim, H. J., & Oh, D. K. (2005). Purification and characterization of an l-arabinose isomerase from an isolated strain of Geobacillus thermodenitrificans producing d-tagatose. Journal of Biotechnology,120, 162–173.
15. Jorgensen, F., Hansen, O. C., & Stougaard, P. (2004). Enzymatic conversion of d-galactose to d-tagatose: heterologous expression and characterisation of a thermostable l-arabinose isomerase from Thermoanaerobacter mathranii.Applied Microbiology and Biotechnology,64, 816–822.
16. Kim, B. C., Lee, Y. H., Lee, H. S., Lee, D. W., Choe, E. A., & Pyun, Y. R. (2002). Cloning, expression and characterization of l-arabinose isomerase from Thermotoga neapolitana: bioconversion of d-galactose to d-tagatose using the enzyme. FEMS Microbiology Letters,212, 121–126.
17. Lee, D. W., Jang, H. J., Choe, E. A., Kim, B. C., Lee, S. J., Kim, S. B., Hong, Y., & Pyun, Y. R. (2004). Characterization of a thermostable l-arabinose (d-galactose) isomerase from the hyperthermophilic eubacterium Thermotoga maritima.Applied and Environmental Microbiology,70, 1397–1404.
18. Lee, S. J., Lee, D. W., Choe, E. A., Hong, Y. H., Kim, S. B., Kim, B. C., & Pyun, Y. R. (2005). Characterization of a thermoacidophilic l-arabinose isomerase from Alicyclobacillus acidocaldarius: role of Lys-269 in pH optimum. Applied and Environmental Microbiology,71, 7888–7896.
19. Xu, Z., Li, S., Feng, X. H., Zhan, Y. J., & Xu, H. (2014). Function of aspartic acid residues in optimum pH control of l-arabinose isomerase from Lactobacillus fermentum.Applied Microbiology and Biotechnology,98, 3987–3996.
20. Lee, S. J., Lee, Y. J., Kim, S. B., Kim, S. K., & Lee, D. W. (2012). Homologous alkalophilic and acidophilic l-arabinose isomerases reveal region-specific contributions to the pH dependence of activity and stability. Applied and Environmental Microbiology,78, 8813–8816.
21. Kim, P., Yoon, S. H., Seo, M. J., Oh, D. K., & Choi, J. H. (2001). Improvement of tagatose conversion rate by genetic evolution of thermostable galactose isomerase. Biotechnology and Applied Biochemistry,34, 99–102.
22. Kim, B. J., Hong, S. H., Shin, K. C., Jo, Y. S., & Oh, D. K. (2014). Characterization of a F280N variant of l-arabinose isomerase from Geobacillus thermodenitrificans identified as a d-galactose isomerase. Applied Microbiology and Biotechnology,98, 9271–9281.
23. Fan, C., Liu, K., Zhang, T., Zhou, L., Xue, D., Jiang, B., & Mu, W. (2014). Biochemical characterization of a thermostable l-arabinose isomerase from a thermoacidophilic bacterium, Alicyclobacillus hesperidum URH17-3–68. Journal of Molecular Catalysis B-Enzymatic,102, 120–126.
24. Cheng, L., Mu, W., & Jiang, B. (2010). Thermostable l-arabinose isomerase from Bacillus stearothermophilus IAM 11001 for d-tagatose production: gene cloning, purification and characterisation. Journal of the Science of Food and Agriculture,90, 1327–1333.
25. Zhou, X., & Wu, J. C. (2012). Heterologous expression and characterization of Bacillus coagulansl-arabinose isomerase. World Journal of Microbiology & Biotechnology,28, 2205–2212.
26. Cheng, L., Mu, W., Zhang, T., & Jiang, B. (2010). An l-arabinose isomerase from Acidothermus cellulolytics ATCC 43068: cloning, expression, purification, and characterization. Applied Microbiology and Biotechnology,86, 1089–1097.
27. Li, Y., Zhu, Y., Liu, A., & Sun, Y. (2011). Identification and characterization of a novel l-arabinose isomerase from Anoxybacillus flavithermus useful in d-tagatose production. Extremophiles,15, 441–450.
28. Manjasetty, B. A., & Chance, M. R. (2006). Crystal structure of Escherichia coli l-arabinose isomerase (ECAI), the putative target of biological tagatose production. Journal of Molecular Biology,360, 297–309.
29. Rhimi, M., Aghajari, N., Juy, M., Chouayekh, H., Maguin, E., Haser, R., & Bejar, S. (2009). Rational design of Bacillus stearothermophilus US100 l-arabinose isomerase: potential applications for d-tagatose production. Biochimie,91, 650–653.
30. Oh, D. K., Oh, H. J., Kim, H. J., Cheon, J., & Kim, P. (2006). Modification of optimal pH in l-arabinose isomerase from Geobacillus stearothermophilus for d-galactose isomerization. Journal of Molecular Catalysis B-Enzymatic,43, 108–112.
31. Oh, H. J., Kim, H. J., & Oh, D. K. (2006). Increase in d-tagatose production rate by site-directed mutagenesis of l-arabinose isomerase from Geobacillus thermodenitrificans.Biotechnology Letters,28, 145–149.