Improvement in Mouse iPSC Induction by Rab32 Reveals the Importance of Lipid Metabolism during Reprogramming

Scientific Reports

Volumn 5, Issue , 2015, Pages

  Pei, Yangli ^a   Yue, Liang ^a   Zhang, Wei ^a   Wang, Yanliang ^a   Wen, Bingqiang ^a   Zhong, Liang ^a   Xiang, Jinzhu ^a   Li, Junhong ^a   Zhang, Shaopeng ^a   Wang, Hanning ^a   Mu, Haiyuan ^a   Wei, Qingqing ^a   Han, Jianyong ^a  

^a CHINA AGRICULTURAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

RAB PROTEIN; RAB32 PROTEIN, MOUSE; TRANSCRIPTION FACTOR;

ANIMAL; AUTOPHAGY; CELL DIFFERENTIATION; CYTOLOGY; GENE EXPRESSION; GENETICS; INDUCED PLURIPOTENT STEM CELL; LIPID METABOLISM; METABOLISM; MOUSE; NUCLEAR REPROGRAMMING; PHAGOSOME;

ANIMALS; AUTOPHAGY; CELL DIFFERENTIATION; CELLULAR REPROGRAMMING; GENE EXPRESSION; INDUCED PLURIPOTENT STEM CELLS; LIPID METABOLISM; MICE; PHAGOSOMES; RAB GTP-BINDING PROTEINS; TRANSCRIPTION FACTORS;

EID: 84947475040     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep16539     Document Type: Article

References (40)

1. Takahashi, K. et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. cell 131, 861-872 (2007).
2. Takahashi, K. & Yamanaka, S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. cell 126, 663-676 (2006).
3. Zhao, X. Y. et al. iPS cells produce viable mice through tetraploid complementation. nature 461, 86-90 (2009).
4. Kang, L., Wang, J., Zhang, Y., Kou, Z. & Gao, S. iPS cells can support full-term development of tetraploid blastocyst-complementedembryos. Cell Stem Cell 5, 135-138 (2009).
5. Boland, M. J. et al. Adult mice generated from induced pluripotent stem cells. nature 461, 91-94 (2009).
6. Panopoulos, A. D. et al. The metabolome of induced pluripotent stem cells reveals metabolic changes occurring in somatic cell reprogramming. Cell Res 22, 168-177 (2012).
7. Chin, M. H. et al. Induced pluripotent stem cells and embryonic stem cells are distinguished by gene expression signatures. Cell Stem Cell 5, 111-123 (2009).
8. Heng, J. C. et al. The nuclear receptor Nr5a2 can replace Oct4 in the reprogramming of murine somatic cells to pluripotent cells. Cell Stem Cell 6, 167-174 (2010).
9. Han, J. et al. Tbx3 improves the germ-line competency of induced pluripotent stem cells. nature 463, 1096-1100 (2010).
10. O'Malley, J. et al. High-resolution analysis with novel cell-surface markers identifies routes to iPS cells. nature 499, 88-91 (2013).
11. Cao, S., Loh, K., Pei, Y., Zhang, W. & Han, J. Overcoming barriers to the clinical utilization of iPSCs: reprogramming efficiency, safety and quality. Protein Cell 3, 834-845 (2012).
12. Feng, B., Ng, J. H., Heng, J. C. & Ng, H. H. Molecules that promote or enhance reprogramming of somatic cells to induced pluripotent stem cells. Cell Stem Cell 4, 301-312 (2009).
13. Liu, K. et al. KSR-based medium improves the generation of high-quality mouse iPS cells. PLoS One 9, e105309 (2014).
14. Markoulaki, S. et al. Transgenic mice with defined combinations of drug-inducible reprogramming factors. Nat Biotechnol 27, 169-171 (2009).
15. Bui, M. et al. Rab32 modulates apoptosis onset and mitochondria-associated membrane (MAM) properties. J Biol Chem 285, 31590-31602 (2010).
16. Alto, N. M., Soderling, J. & Scott, J. D. Rab32 is an A-kinase anchoring protein and participates in mitochondrial dynamics. J Cell Biol 158, 659-668 (2002).
17. Bultema, J. J. & Di Pietro, S. M. Cell type-specific Rab32 and Rab38 cooperate with the ubiquitous lysosome biogenesis machinery to synthesize specialized lysosome-related organelles. Small GTPases 4, 16-21 (2013).
18. Park, M., Serpinskaya, A. S., Papalopulu, N. & Gelfand, V. I. Rab32 regulates melanosome transport in Xenopus melanophores by protein kinase a recruitment. Curr Biol 17, 2030-2034 (2007).
19. Tamura, K. et al. Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol Biol Cell 20, 2900-2908 (2009).
20. Wasmeier, C. et al. Rab38 and Rab32 control post-Golgi trafficking of melanogenic enzymes. J Cell Biol 175, 271-281 (2006).
21. Wang, C., Liu, Z. & Huang, X. Rab32 is important for autophagy and lipid storage in Drosophila. PLoS One 7, e32086 (2012).
22. Hirota, Y. & Tanaka, Y. A small GTPase, human Rab32, is required for the formation of autophagic vacuoles under basal conditions. Cell Mol Life Sci 66, 2913-2932 (2009).
23. Knobloch, M. et al. Metabolic control of adult neural stem cell activity by Fasn-dependent lipogenesis. nature 493, 226-230 (2013).
24. Demine, S. et al. Macroautophagy and cell responses related to mitochondrial dysfunction, lipid metabolism and unconventional secretion of proteins. Cells 1, 168-203 (2012).
25. Singh, R. et al. Autophagy regulates lipid metabolism. nature 458, 1131-1135 (2009).
26. Nazio, F. et al. mTOR inhibits autophagy by controlling ULK1 ubiquitylation, self-association and function through AMBRA1 and TRAF6. Nat Cell Biol 15, 406-416 (2013).
27. Huang, J., Dibble, C. C., Matsuzaki, M. & Manning, B. D. The TSC1-TSC2 complex is required for proper activation of mTOR complex 2. Mol Cell Biol 28, 4104-4115 (2008).
28. Chen, T. et al. Rapamycin and other longevity-promoting compounds enhance the generation of mouse induced pluripotent stem cells. Aging Cell 10, 908-911 (2011).
29. Bennett, B. L. et al. SP600125, an anthrapyrazolone inhibitor of Jun N-terminal kinase. Proc Natl Acad Sci USA 98, 13681-13686 (2001).
30. Halvorson, D. L. & McCune, S. A. Inhibition of fatty acid synthesis in isolated adipocytes by 5-(tetradecyloxy)-2-furoic acid. Lipids 19, 851-856 (1984).
31. Hutagalung, A. H. & Novick, P. J. Role of Rab GTPases in membrane traffic and cell physiology. Physiol Rev 91, 119-149 (2011).
32. Ao, X., Zou, L. & Wu, Y. Regulation of autophagy by the Rab GTPase network. Cell Death Differ 21, 348-358 (2014).
33. Marks, M. S. Darkness descends with two Rabs. J Cell Biol 175, 199-200 (2006).
34. Zhang, J., Nuebel, E., Daley, G. Q., Koehler, C. M. & Teitell, M. A. Metabolic regulation in pluripotent stem cells during reprogramming and self-renewal. Cell Stem Cell 11, 589-595 (2012).
35. Muthusamy, T., Mukherjee, O., Menon, R., Megha, P. B. & Panicker, M. M. A method to identify and isolate pluripotent human stem cells and mouse epiblast stem cells using lipid body-associated retinyl ester fluorescence. Stem Cell Reports 3, 169-184 (2014).
36. Vazquez-Martin, A. et al. The mitochondrial H(+)-ATP synthase and the lipogenic switch: new core components of metabolic reprogramming in induced pluripotent stem (iPS) cells. Cell Cycle 12, 207-218 (2013).
37. Kim, C. W. et al. Induced polymerization of mammalian acetyl-CoA carboxylase by MIG12 provides a tertiary level of regulation of fatty acid synthesis. Proc Natl Acad Sci USA 107, 9626-9631 (2010).
38. Feng, B. et al. Reprogramming of fibroblasts into induced pluripotent stem cells with orphan nuclear receptor Esrrb. Nat Cell Biol 11, 197-203 (2009).
39. Jiang, J. et al. A core Klf circuitry regulates self-renewal of embryonic stem cells. Nat Cell Biol 10, 353-360 (2008).
40. Hasuwa, H. et al. Transgenic mouse sperm that have green acrosome and red mitochondria allow visualization of sperm and their acrosome reaction in vivo. Exp Anim 59, 105-107 (2010).