Extracting Cell Stiffness from Real-Time Deformability Cytometry: Theory and Experiment

Biophysical Journal

Volumn 109, Issue 10, 2015, Pages 2023-2036

  Mietke, Alexander ^a   Otto, Oliver ^a   Girardo, Salvatore ^a   Rosendahl, Philipp ^a   Taubenberger, Anna ^a   Golfier, Stefan ^a   Ulbricht, Elke ^a   Aland, Sebastian ^a   Guck, Jochen ^a   Fischer Friedrich, Elisabeth ^b,c  

^a DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

^b MAX PLANCK INSTITUTE OF MOLECULAR CELL BIOLOGY AND GENETICS   (Germany)

^c MAX PLANCK INSTITUT FÜR PHYSIK KOMPLEXER SYSTEME   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CELL SEPARATION; CELL SHAPE; ELASTICITY; HUMAN; MECHANICAL STRESS; MICROFLUIDICS; PROCEDURES; THEORETICAL MODEL; TUMOR CELL LINE;

CELL LINE, TUMOR; CELL SEPARATION; CELL SHAPE; ELASTICITY; HUMANS; MICROFLUIDICS; MODELS, THEORETICAL; STRESS, MECHANICAL;

EID: 84947269621     PISSN: 00063495     EISSN: 15420086     Source Type: Journal    
DOI: 10.1016/j.bpj.2015.09.006     Document Type: Article

References (48)

1. A.E. Ekpenyong, and G. Whyte J. Guck Viscoelastic properties of differentiating blood cells are fate- and function-dependent PLoS One 7 2012 e45237
2. F. Lautenschläger, and S. Paschke J. Guck The regulatory role of cell mechanics for migration of differentiating myeloid cells Proc. Natl. Acad. Sci. USA 106 2009 15696 15701
3. H.T.K. Tse, and D.R. Gossett D.D. Carlo Quantitative diagnosis of malignant pleural effusions by single-cell mechanophenotyping Sci. Transl. Med. 5 2013 212ra163
4. S.-Y. Tee, and J. Fu P.A. Janmey Cell shape and substrate rigidity both regulate cell stiffness Biophys. J. 100 2011 L25 L27
5. S. Suresh Biomechanics and biophysics of cancer cells Acta Biomater. 3 2007 413 438
6. B. Lincoln, and H.M. Erickson J. Guck Deformability-based flow cytometry Cytometry A 59 2004 203 209
7. J. Guck, and S. Schinkinger C. Bilby Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence Biophys. J. 88 2005 3689 3698
8. T.W. Remmerbach, and F. Wottawah J. Guck Oral cancer diagnosis by mechanical phenotyping Cancer Res. 69 2009 1728 1732
9. S.C. Hur, H.T.K. Tse, and D. Di Carlo Sheathless inertial cell ordering for extreme throughput flow cytometry Lab Chip 10 2010 274 280
10. C. Pfafferott, G.B. Nash, and H.J. Meiselman Red blood cell deformation in shear flow. Effects of internal and external phase viscosity and of in vivo aging Biophys. J. 47 1985 695 704
11. V. Swaminathan, and K. Mythreye R. Superfine Mechanical stiffness grades metastatic potential in patient tumor cells and in cancer cell lines Cancer Res. 71 2011 5075 5080
12. G. Wang, and W. Mao T. Sulchek Stiffness dependent separation of cells in a microfluidic device PLoS One 8 2013 e75901
13. D.R. Gossett, and H.T.K. Tse D. Di Carlo Hydrodynamic stretching of single cells for large population mechanical phenotyping Proc. Natl. Acad. Sci. USA 109 2012 7630 7635
14. W. Xu, and R. Mezencev T. Sulchek Cell stiffness is a biomarker of the metastatic potential of ovarian cancer cells PLoS One 7 2012 e46609
15. P.A. Pullarkat, P.A. Fernández, and A. Ott Rheological properties of the Eukaryotic cell cytoskeleton Phys. Rep. 449 2007 29 53
16. S. Byun, and S. Son S.R. Manalis Characterizing deformability and surface friction of cancer cells Proc. Natl. Acad. Sci. USA 110 2013 7580 7585
17. O. Otto, and P. Rosendahl J. Guck Real-time deformability cytometry: on-the-fly cell mechanical phenotyping Nat. Methods 12 2015 199 202 4, 202 (advance online publication)
18. J.R. Lange, and J. Steinwachs B. Fabry Microconstriction arrays for high-throughput quantitative measurements of cell mechanical properties Biophys. J. 109 2015 26 34
19. D.A. Fedosov, H. Noguchi, and G. Gompper Multiscale modeling of blood flow: from single cells to blood rheology Biomech. Model. Mechanobiol. 13 2014 239 258
20. D. Abreu, and M. Levant U. Seifert Fluid vesicles in flow Adv. Colloid Interface Sci. 208 2014 129 141
21. D.A. Fedosov, M. Peltomäki, and G. Gompper Deformation and dynamics of red blood cells in flow through cylindrical microchannels Soft Matter 10 2014 4258 4267
22. G. Coupier, and A. Farutin C. Misbah Shape diagram of vesicles in Poiseuille flow Phys. Rev. Lett. 108 2012 178106
23. H. Noguchi, and G. Gompper Shape transitions of fluid vesicles and red blood cells in capillary flows Proc. Natl. Acad. Sci. USA 102 2005 14159 14164
24. C. Pozrikidis Numerical simulation of cell motion in tube flow Ann. Biomed. Eng. 33 2005 165 178
25. X.-Q. Hu, A.-V. Salsac, and D. Barthès-Biesel Flow of a spherical capsule in a pore with circular or square cross-section J. Fluid Mech. 705 2012 176 194
26. S. Kuriakose, and P. Dimitrakopoulos Motion of an elastic capsule in a square microfluidic channel Phys. Rev. E Stat. Nonlin. Soft Matter Phys. 84 2011 011906
27. D. Barthès-Biesel Motion of a spherical microcapsule freely suspended in a linear shear flow J. Fluid Mech. 100 1980 831 853
28. M.J. Lighthill Pressure-forcing of tightly fitting pellets along fluid-filled elastic tubes J. Fluid Mech. 34 1968 113 143
29. J.M. Fitz-Gerald Mechanics of red-cell motion through very narrow capillaries Proc. R. Soc. Lond. B Biol. Sci. 174 1969 193 227
30. Haberman, L., and R. Sayre. 1958. Motion of Rigid and Fluid Spheres in Stationary and Moving Liquids inside Cylindrical Tubes (David W. Taylor Model Basin. Report). David Taylor, editor. Department of the Navy, Bethesda, MD.
31. H. Bruus Theoretical Microfluidics 2008 Oxford University Press Oxford, United Kingdom
32. R. Huebscher Friction equivalents for round, square and rectangular ducts ASHVE Transactions 54 1948 101 118
33. J. Happel, and H. Brenner Low Reynolds Number Hydrodynamics 1973 Nordhoff International Chatsworth, CA
34. L. Landau, and E. Lifshitz Theory of Elasticity 1986 Elsevier Amsterdam
35. A.I. Lurie Theory of Elasticity 2005 Springer New York
36. R. Ananthakrishnan, and J. Guck J. Käs Quantifying the contribution of actin networks to the elastic strength of fibroblasts J. Theor. Biol. 242 2006 502 516
37. A. Ugural Stresses in Plates and Shells 1999 McGraw-Hill New York
38. G. Salbreux, G. Charras, and E. Paluch Actin cortex mechanics and cellular morphogenesis Trends Cell Biol. 22 2012 536 545
39. E. Fischer-Friedrich, and A.A. Hyman J. Helenius Quantification of surface tension and internal pressure generated by single mitotic cells Sci. Rep. 4 2014 6213
40. H. Berthoumieux, and J.-L. Maître G. Salbreux Active elastic thin shell theory for cellular deformations New J. Phys. 16 2014 065005
41. J. Domke, and M. Radmacher Measuring the elastic properties of thin polymer films with the atomic force microscope Langmuir 14 1998 3320 3325
42. M. Radmacher, and M. Fritz P.K. Hansma Measuring the viscoelastic properties of human platelets with the atomic force microscope Biophys. J. 70 1996 556 567
43. P. Kollmannsberger, and B. Fabry Linear and nonlinear rheology of living cells Annu. Rev. Mater. Res. 41 2011 75 97
44. M.J. Rosenbluth, W.A. Lam, and D.A. Fletcher Force microscopy of nonadherent cells: a comparison of leukemia cell deformability Biophys. J. 90 2006 2994 3003
45. A.F. Straight, and A. Cheung T.J. Mitchison Dissecting temporal and spatial control of cytokinesis with a myosin II Inhibitor Science 299 2003 1743 1747
46. J.-Y. Tinevez, and U. Schulze E. Paluch Role of cortical tension in bleb growth Proc. Natl. Acad. Sci. USA 106 2009 18581 18586
47. V.T. Nayar, and J.D. Weiland A.M. Hodge Elastic and viscoelastic characterization of agar J. Mech. Behav. Biomed. Mater. 7 2012 60 68
48. A. Pietuch, and B.R. Brückner A. Janshoff Elastic properties of cells in the context of confluent cell monolayers: impact of tension and surface area regulation Soft Matter 9 2013 11490 11502