Engineered hydrophobic pocket of (S)-selective arylmalonate decarboxylase variant by simultaneous saturation mutagenesis to improve catalytic performance

Bioscience, Biotechnology and Biochemistry

Volumn 79, Issue 12, 2015, Pages 1965-1971

  Yoshida, Shosuke ^a   Enoki, Junichi ^a   Kourist, Robert ^b   Miyamoto, Kenji ^a  

^a KEIO UNIVERSITY   (Japan)

^b RUHR UNIVERSITY BOCHUM   (Germany)

Author keywords

(S) profen; (S) selective arylmalonate decarboxylase; Activity improvement; Saturation mutagenesis

Indexed keywords

CARBOXYLATION; DRUG PRODUCTS; HYDROPHOBICITY;

(S)-PROFEN; ACTIVE SITE RESIDUES; CATALYTIC EFFICIENCIES; CATALYTIC PERFORMANCE; DECARBOXYLASES; HYDROPHOBIC POCKETS; SATURATION MUTAGENESIS; WILD TYPES;

MUTAGENESIS;

CARBOXYLYASE; MALONATE DECARBOXYLASE; MALONIC ACID; MALONIC ACID DERIVATIVE;

BIOCATALYSIS; CHEMICAL PHENOMENA; CHEMICAL STRUCTURE; CHEMISTRY; DIRECTED MOLECULAR EVOLUTION; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; GENETICS; METABOLISM; MUTATION; PROTEIN ENGINEERING; SITE DIRECTED MUTAGENESIS; STEREOISOMERISM;

BIOCATALYSIS; CARBOXY-LYASES; CATALYTIC DOMAIN; DIRECTED MOLECULAR EVOLUTION; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; MALONATES; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROTEIN ENGINEERING; STEREOISOMERISM; SUBSTRATE SPECIFICITY;

EID: 84946943993     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1080/09168451.2015.1060844     Document Type: Article

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