Optimization of cellulase production by Enhydrobacter sp. ACCA2 and its application in biomass saccharification

Frontiers in Microbiology

Volumn 6, Issue OCT, 2015, Pages

  Premalatha, Nagaiah ^a   Gopal, Nellaiappan O ^a   Jose, Polpass Arul ^a   Anandham, Rangasamy ^a   Kwon, Soon Wo ^b  

^a TAMIL NADU AGRICULTURAL UNIVERSITY   (India)

^b National Institute of Agricultural Science   (South Korea)

Author keywords

Carboxy methyl cellulose; Cellulase; Central composite design; Response surface methodology; Saccharification

Indexed keywords

AMMONIUM SULFATE; CARBOXYMETHYLCELLULOSE; CELLULASE; DIPOTASSIUM HYDROGEN PHOSPHATE; MAGNESIUM SULFATE; PEPTONE; RNA 16S; SODIUM CHLORIDE;

ARTICLE; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; BIOMASS FERMENTATION; CARBON SOURCE; CULTURE MEDIUM; ENHYDROBACTER; ENZYME ACTIVITY; FERMENTATION OPTIMIZATION; INCUBATION TIME; NONHUMAN; PH; RESPONSE SURFACE METHOD; RNA GENE; SACCHARIFICATION; TEMPERATURE;

EID: 84946828496     PISSN: None     EISSN: 1664302X     Source Type: Journal    
DOI: 10.3389/fmicb.2015.01046     Document Type: Article

References (58)

1. Acharya, S., and Chaudhary, A. (2011). Effect of nutritional and environmental factors on cellulases activity by thermophilic bacteria isolated from hot spring. J. Sci. Ind. Res. 70, 142-148.
2. Agbor, V. B., Cicek, N., Sparling, R., Berlin, A., and Levin, D. B. (2011). Biomass pretreatment: fundamentals toward application. Biotechnol. Adv. 29, 675-685. doi: 10.1016/j.biotechadv.2011.05.005
3. Beauchemin, K. A., Rode, L. M., and Sewalt, V. J. H. (1995). Fibrolytic enzymes increase fiber digestibility and growth rate of steers fed dry forages. Can. J. Anim. Sci. 75, 641-644. doi: 10.4141/cjas95-096
4. Chakraborty, S., Khopadea, A., Kokarea, C. K., and Mahadika, K. S. (2009). Isolation and characterization of novel-amylase from marine Streptomyces sp. D1. J. Mol. Catal. B Enzy. 58, 17-23. doi: 10.1016/j.molcatb.2008.10.011
5. Chang, M. C. Y. (2007). Harnessing energy from plant biomass. Curr. Opin. Chem. Biol. 11, 677-684. doi: 10.1016/j.cbpa.2007.08.039
6. Chen, H., Wu, M., Chen, Z., Wang, M., Lin, J., and Yang, L. (2013). Enhancing production of a 24-membered ring macrolide compound by a marine bacterium using response surface methodology. J. Zhejiang Univ. Sci. B 14, 346-354. doi: 10.1631/jzus.B1200153
7. Dalmaso, G. Z. L., Ferreira, D., and Vermelho, A. B. (2015). Marine Extremophiles: a source of hydrolases for biotechnological applications. Mar. Drugs 13, 1925-1965. doi: 10.3390/md13041925
8. Dantur, K. I., Enrique, R., Welin, B., and Castagnaro, A. P. (2015). Isolation of cellulolytic bacteria from the intestine of Diatraea saccharalis larvae and evaluation of their capacity to degrade sugarcane biomass. AMB Exp. 5, 15. doi: 10.1186/s13568-015-0101-z
9. Deka, D., Bhargavi, P., Sharma, A., Goyal, D., Jawed, M., and Goyal, A. (2011). Enhancement of cellulase activity from a new strain of Bacillus subtilis by medium optimization and analysis with various cellulosic substrates. Enzy. Res. 2011:151656. doi: 10.4061/2011/151656
10. Deka, D., Das, S. P., Sahoo, N., Das, D., Jawed, M., Goyal, D., et al. (2013). Enhanced cellulase production from Bacillus subtilis by optimizing physical parameters for bioethanol production. ISRN Biotechnol. 2013:965310. doi: 10.5402/2013/965310
11. Eida, M. F., Nagaoka, T., Wasaki, J., and Kouno, K. (2012). Isolation and characterization of cellulose-decomposing bacteria inhabiting sawdust and coffee residue composts. Microbes Environ. 27, 226-233. doi: 10.1264/jsme2.ME11299
12. Elisashvili, V. I., Khardziani, T. S., Tsiklauri, N. D., and Kachlishvili, E. T. (1999). Cellulase and xylanase activities in higher basidiomycetes. Biochemistry (Mosc) 64, 718-722.
13. Fang, G. R., Li, J. J., Cheng, X., and Cui, Z. J. (2012). Performance and spatial succession of a full-scale anaerobic plant treating high-concentration cassava bioethanol wastewater. J. Microbiol. Biotechnol. 22, 1148-1154. doi: 10.4014/jmb.1202.02015
14. Fujita, Y., Takahashi, S., Ueda, M., Tanaka, A., Okada, H., Morikawa, Y., et al. (2002). Direct and efficient production of ethanol from cellulosic material with a yeast strain displaying cellulolytic enzymes. Appl. Environ. Microbiol. 68, 5136-5141. doi: 10.1128/AEM.68.10.5136-5141.2002
15. Geetha, K., and Gunasekaran, P. (2010). Optimization of nutrient medium containing agricultural waste for xylanase production by Bacillus pumilus B20. Biotechnol. Bioprocess. Eng. 15, 882-889. doi: 10.1007/s12257-009-3094-0
16. Ghio, S., Lorenzo, G. S. D., Lia, V., Talia, P., Cataldi, A., Grasso, D., et al. (2012). Isolation of Paenibacillus sp. and Variovorax sp. strains from decaying woods and characterization of their potential for cellulose deconstruction. Int. J. Biochem. Mol. Biol. 3, 352-364.
17. Goyal, V., Mittal, A., Bhuwal, A. K., Singh, G., Yadav, A., and Aggarwal, N. K. (2014). Parametric optimization of cultural conditions for carboxymethyl cellulase production using pretreated rice straw by Bacillus sp. 313SI under stationary and shaking conditions. Biotechnol. Res. Int. 2014:651839. doi: 10.1155/2014/651839
18. Harshvardhan, K., Mishra, A., and Jha, B. (2013). Purification and characterization of cellulase from a marine Bacillus sp. H1666: a potential agent for single step saccharification of seaweed biomass. J. Mol. Catal. B Enzy. 93, 51-56.
19. Hendricks, C. W., Doyle, J. D., and Hugley, B. (1995). A new solid medium for enumerating cellulose-utilizing bacteria in soil. Appl. Environ. Microbiol. 61, 2016-2019.
20. Ji, S., Wang, S., Tan, Y., Chen, X., Schwarz, W., and Li, F. (2012). An untapped bacterial cellulolytic community enriched from coastal marine sediment under anaerobic and thermophilic conditions. FEMS Microbiol. Lett. 335, 39-46. doi: 10.1111/j.1574-6968.2012.02636.x
21. Jose, P. A., and Jebakumar, S. R. D. (2014). Successive nonstatistical and statistical approaches for the improved antibiotic activity of rare actinomycete Nonomuraea sp. JAJ18. Biomed Res. Int. 2014:906097. doi: 10.1155/2014/906097
22. Jyotsna, K. P. P., Vijayalakshmi, K., Prasanna, N. D., and Shaheen, S. K. (2010). Isolation and characterization of cellulose producing Lysinibacillus sphaericus MTCC No 9468 from gut of Eisenia Foetida. Bioscan 6, 325-327.
23. Kawamura, Y., Fujiwara, N., Naka, T., Mitani, A., Kubota, H., Tomida, J., et al. (2012). Genus Enhydrobacter Staley et al., 1987 should be recognized as a member of the family Rhodospirillaceae within the class Alphaproteobacteria. Microbiol. Immunol. 56, 21-26. doi: 10.1111/j.1348-0421.2011.00401.x
24. Kim, H.-J., Lee, Y.-J., Gao, W., Chung, C.-H., Son, C.-W., and Lee, J.-W. (2011). Statistical optimization of fermentation conditions and comparison of their influences on production of cellulases by a psychrophilic marine bacterium. Psychrobacter aquimaris LBH-10 using orthogonal array method. Biotechnol. Bioprocess. Eng. 16, 542-548. doi: 10.1007/s12257-010-0457-5
25. Kim, O. S., Cho, Y. J., Lee, K., Yoon, S. H., Kim, M., Na, H., et al. (2012). Introducing EzTaxon: a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species. Int. J. Syst. Evol. Microbiol. 62, 716-721. doi: 10.1099/ijs.0.038075-0
26. Kirk, O., Borchert, T. V., and Fuglsang, C. C. (2002). Industrial enzyme applications. Curr. Opin. Biotechnol. 13, 345-351. doi: 10.1016/S0958-1669(02)00328-2
27. Lai, D., Deng, L., Li, J., Liao, B., Guo, Q. X., and Fu, Y. (2011). Hydrolysis of cellulose into glucose by magnetic solid acid. ChemSusChem 4, 55-58. doi: 10.1002/cssc.201000300
28. Lane, D. J. (1991). "6S/23S rRNA sequencing". in Nucleic Acid Techniques in Bacterial Systematics, eds E. Stackebrandt and M. Goodfellow (New York: John Wiley & Sons), 115-175.
29. Lee, S. H., Oh, D. H., Jung, J. Y., Kim, J. C., and Jeon, C. O. (2012). Comparative ocular microbial communities in humans with and without blepharitis. Invest. Ophthalmol. Vis. Sci. 53, 5585-5593. doi: 10.1167/iovs.12-9922
30. Lee, Y. J., Kim, B. K., Lee, B. H., Jo, K. I., Lee, N. K., Chung, C. H., et al. (2008). Purification and characterization of cellulase produced by Bacillus amyoliquefaciens DL-3 utilizing rice hull. Bioresour. Technol. 99, 378-386. doi: 10.1016/j.biortech.2006.12.013
31. Lewis, G. E., Hunt, C. W., Sanchez, W. K., Treacher, R., Pritchard, G. T., and Feng, P. (1996). Effect of direct-fed fibrolytic enzymes o the digestive characteristics of a forage-based diet fed to beef steers. Anim. Sci. 74, 3020-3028.
32. Li, P. P., Wang, X. J., and Cui, Z. J. (2012). Survival and performance of two cellulose-degrading microbial systems inoculated into wheat straw-amended soil. J. Microbiol. Biotechnol. 22, 126-132. doi: 10.4014/jmb.1102.02021
33. Li, W., Zhang, W. W., Yang, M. M., and Chen, Y. L. (2008). Cloning of the thermostable cellulase gene from newly isolated Bacillus subtilis and its expression in Escherichia coli. Mol. Biotechnol. 40, 195-201. doi: 10.1007/s12033-008-9079-y
34. Liu, J. M., Xin, X. J., Li, C. X., Xu, J. H., and Bao, J. (2012). Cloning of thermostable cellulase genesof Clostridium thermocellum and their secretive expression in Bacillus subtilis. Appl. Biochem. Biotechnol. 166, 652-662. doi: 10.1007/s12010-011-9456-z
35. Maki, M. L., Broere, M., Leung, K. T., and Qin, W. (2011). Characterization of some efficient cellulase producing bacteria isolated from paper mill sludges and organic fertilizers Int. J. Biochem. Mol. Biol. 2, 146-154.
36. Rajeswari, P., Jose, P. A., Amiya, R., and Jebakumar, S. R. D. (2015). Characterization of saltern based Streptomyces sp. and statistical media optimization for its improved antibacterial activity. Front. Microbiol. 5:753. doi: 10.3389/fmicb.2014.00753
37. Rastogi, G., Bhalla, A., Adhikari, A., Bischoff, K. M., Hughes, S. R., Christopher, L. P., et al. (2010). Characterization of thermostable cellulases produced by Bacillus and Geobacillus strains. Bioresour. Technol. 101, 8798-8806. doi: 10.1016/j.biortech.2010.06.001
38. Sadhu, S., Ghosh, P. K., Aditya, G., and Maiti, T. K. (2014). Optimization and strain improvement by mutation for enhanced cellulase production by Bacillus sp. (MTCC10046) isolated from cow dung. J. King Saud. Univ. Sci. 26, 323-332. doi: 10.1016/j.jksus.2014.06.001
39. Sadhu, S., Ghosh, P. K., De, T. K., and Maiti, T. K. (2013). Optimization of cultural condition and synergistic effect of lactose with carboxymethyl cellulose on cellulase production by Bacillus sp. isolated from fecal matter of elephant (elephas maximus). Adv. Microbiol. 3, 280-288. doi: 10.4236/aim.2013.33040
40. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989). Molecular Cloning. A Laboratory Manual. Cold Spring: Harbor Laboratory Press.
41. Santhi, V. S., Gupta, A., Saranya, S., and Jebakumar, S. R. D. (2014a). A novel marine bacterium Isoptericola sp. JS-C42 with the ability to saccharifying the plant biomasses for the aid in cellulosic ethanol production. Biotechnol. Rep. 1-2, 8-14.
42. Santhi, V. S., Bhagat, A. K., Saranya, S., Govindarajan, G., and Jebakumar, S. R. D. (2014b). Seaweed (Eucheuma cottonii) associated microorganisms, a versatile enzyme source for the lignocellulosic biomass processing. Int. Biodeterior. Biodegradation 96, 144-151. doi: 10.1016/j.ibiod.2014.08.007
43. Sethi, S., Datta, A., Gupta, B. L., and Gupta, S. (2013). Optimization of cellulase production from bacteria isolated from soil. ISRN Biotechnol. 2013:985685. doi: 10.5402/2013/985685
44. Sharma, P., Sharma, S., Maurya, R. K., De, T. D., Thomas, T., Lata, S., et al. (2014). Salivary glands harbor more diverse microbial communities than gut in Anopheles culicifacies. Parasit. Vectors 7, 235. doi: 10.1186/1756-3305-7-235
45. Sindhu, R., Kuttiraja, M., Binod, P., Sukumaran, R. K., and Pandey, A. (2014). Bioethanol production from dilute acid pretreated Indian bamboo variety (Dendrocalamus sp.) by separate hydrolysis and fermentation. Ind. Crop. Prod. 52, 169-176. doi: 10.1016/j.indcrop.2013.10.021
46. Singh, D., and Kaur, G. (2012). Optimization of different process variables for the production of an indolizidine alkaloid, swainsonine from Metarhizium anisopliae. J. Basic Microbiol. 52, 590-597. doi: 10.1002/jobm.201100255
47. Singh, S., Moholkar, V. S., and Goyal, A. (2013). Isolation, identification, and characterization of a cellulolytic Bacillus amyloliquefaciens strain SS35 from rhinoceros dung. ISRN Microbiol. 2013:728134 doi: 10.1155/2013/728134
48. Singh, S., Moholkar, V. S., and Goyal, A. (2014). Optimization of carboxymethylcellulase production from Bacillus amyloliquefaciens SS35. 3 Biotech 4, 411-424. doi: 10.1007/s13205-013-0169-6
49. Soares, F. L. Jr., Melo, I. S., Dias, A. C. F., and Andreote, F. D. (2012). Cellulolytic bacteria from soils in harsh environments. World J. Microbiol. Biotechnol. 228, 2195-2203. doi: 10.1007/s11274-012-1025-2
50. Staley, J. T., Irgens, R. L., and Brenner, D. J. (1987). Enhydrobacter aerosaccus gen. nov., sp. nov., a gas-vacuolated, facultatively anaerobic, heterotrophic rod. Int. J. Syst. Bacteriol. 37, 289-291. doi: 10.1099/00207713-37-3-289
51. Sukumaran, K. R., Singhania, R. R., and Pandey, A. (2005). Microbial cellulases production, applications and challenge. J. Sci. Ind. Res. 64, 834-844.
52. Twomey, L. N., Pluske, J. R., Rowe, J. B., Choct, M., Brown, W., McConnell, M. F., et al. (2003). The effects of increasing levels of soluble non-starch polysaccharides and inclusion of feed enzymes in dog diets on faecal quality and digestibility. Anim. Feed Sci. Technol. 108, 71-82. doi: 10.1016/S0377-8401(03)00161-5
53. Uma, C., Muthulakshmi, C., Gomathi, D., and Gopalakrishnan, V. K. (2010). Fungal invertase as aid for production of ethanol from sugarcane bagasse. Res. J. Microbiol. 5, 980-985. doi: 10.3923/jm.2010.980.985
54. Wang, Y., Fang, X., An, F., Wang, G., and Zhang, X. (2011). Improvement of antibiotic activity of Xenorhabdus bovienii by medium optimization using response surface methodology. Microbial. Cell Fact. 10, 98. doi: 10.1186/1475-2859-10-98
55. Wood, T. M., and Bhat, K. M. (1988). Methods of measuring cellulase activities. Methods Ezy. 160, 87-117. doi: 10.1016/0076-6879(88)60109-1
56. Yang, W., Meng, F., Peng, J., Han, P., Fang, F., Ma, L., et al. (2014). Isolation and identification of a cellulolytic bacterium from the Tibetan pig's intestine and investigation of its cellulase production. Electronic J. Biotechnol. 17, 262-267. doi: 10.1016/j.ejbt.2014.08.002
57. Zambare, V. (2011). Optimization of amylase production from Bacillus sp. using statistics based experimental design. Emir. J. Food Agric. 23, 37-47. doi: 10.9755/ejfa.v23i1.5311
58. Zhang, Y. M., Lu, X. B., Dan, H. B., and Sun, Y. K. (2009). Screening and enzymatic study of a composite microbial system FH3. Braz. Arch. Biol. Technol. 52, 35-43. doi: 10.1590/S1516-89132009000100005