A microfluidic platform for transcription- and amplification-free detection of zepto-mole amounts of nucleic acid molecules

Biosensors and Bioelectronics

Volumn 78, Issue , 2016, Pages 1-6

  Mayr, Reinhard ^a   Haider, Michaela ^a   Thünauer, Roland ^a,c   Haselgrübler, Thomas ^a   Schütz, Gerhard J ^b   Sonnleitner, Alois ^a   Hesse, Jan ^a  

^a Center for Advanced Bioanalysis GmbH   (Austria)

^b VIENNA UNIVERSITY OF TECHNOLOGY   (Austria)

^c UNIVERSITY OF FREIBURG   (Germany)

Author keywords

DNA; Lab on a chip; Microarray; Microfluidic biochip; RNA; Ultra sensitive detection

Indexed keywords

BIOCHIPS; DNA; LAB-ON-A-CHIP; MICROARRAYS; MICROFLUIDICS; NUCLEIC ACIDS; POLYMERASE CHAIN REACTION; PROBES; RNA; TRANSCRIPTION;

ENZYMATIC PROCESS; MICRO FLUIDIC BIOCHIPS; MICROFLUIDIC CHIP; MICROFLUIDIC PLATFORMS; PRE-HYBRIDIZATION; REVERSE TRANSCRIPTION; SINGLE-MOLECULE DETECTION; ULTRA SENSITIVES;

MOLECULES;

DNA; NUCLEIC ACID; RNA;

ARTICLE; CHEMICAL LABELING; CONTROLLED STUDY; DNA DETERMINATION; DNA HYBRIDIZATION; LAB ON A CHIP; LIMIT OF DETECTION; MICROARRAY ANALYSIS; MICROFLUIDICS; NUCLEIC ACID IMMOBILIZATION; RNA ANALYSIS; RNA HYBRIDIZATION; DNA MICROARRAY; FLUORESCENCE; GENETIC PROCEDURES; GENETIC TRANSCRIPTION; GENETICS; ISOLATION AND PURIFICATION; NUCLEIC ACID AMPLIFICATION; NUCLEIC ACID HYBRIDIZATION; PROCEDURES;

BIOSENSING TECHNIQUES; DNA; FLUORESCENCE; LIMIT OF DETECTION; MICROFLUIDICS; NUCLEIC ACID AMPLIFICATION TECHNIQUES; NUCLEIC ACID HYBRIDIZATION; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; RNA; TRANSCRIPTION, GENETIC;

EID: 84946763014     PISSN: 09565663     EISSN: 18734235     Source Type: Journal    
DOI: 10.1016/j.bios.2015.11.013     Document Type: Article

References (33)

1. Acinas S.G., Sarma-Rupavtarm R., Klepac-Ceraj V., Polz M.F. Appl. Env. Microbiol. 2005, 71:8966-8969.
2. Adamski M.G., Gumann P., Baird A.E. PLoS. One 2014, 9:e103917.
3. Álvarez M., Menéndez-Arias L. FEBS J. 2014, 281:342-351.
4. Baker M. Nat. Methods 2012, 9:541-544.
5. Bengtsson M., Hemberg M., Rorsman P., Ståhlberg A. BMC Mol. Biol. 2008, 9:63.
6. Blais J., Lavoie S.B., Giroux S., Bussières J., Lindsay C., Dionne J., Laroche M., Giguère Y., Rousseau F. J. Mol. Diagn. 2015, 17:505-514.
7. Bustin S., Dhillon H.S., Kirvell S., Greenwood C., Parker M., Shipley G.L., Nolan T. Clin. Chem. 2015, 61:202-212.
8. Cocquet J., Chong A., Zhang G., Veitia R.A. Genomics 2006, 88:127-131.
9. Freudenthaler, G., Sonnleitner, M., Sonnleitner, A., 2005. Austrian Patent Office. AT 414 173 B. A 1055/2003.
10. Haddad F., Qin A.X., Giger J.M., Guo H., Baldwin K.M. BMC Biotechnol. 2007, 7.
11. Hernandez F.H., Escriche J.M. The Analyst 1984, 109:1585.
12. Hesse J., Jacak J., Kasper M., Regl G., Eichberger T., Winklmayr M., Aberger F., Sonnleitner M., Schlapak R., Howorka S., Muresan L., Frischauf A.-M., Schütz G.J. Genome Res. 2006, 16:1041-1045.
13. Hesse, J., Jacak, J., Regl, G., Eichberger, T., Aberger, F., Schlapak, R., Howorka, S., Muresan, L., Frischauf, A.-M., Schütz, G.J., 2007. Biomedical Optics (BiOS). International Society for Optics and Photonics. pp. 64440F-64440F.
14. Hesse J., Sonnleitner M., Sonnleitner A., Freudenthaler G., Jacak J., Höglinger O., Schindler H., Schütz G.J. Anal. Chem. 2004, 76:5960-5964.
15. Huang S., Li C., Lin B., Qin J. Lab. Chip 2010, 10:2925.
16. Jacak J., Hesse J., Hesch C., Kasper M., Aberger F., Frischauf A.-M., Sonnleitner M., Freudenthaler G.ünter, Howorka S., Schütz G.J. Proc. SPIE 2005, 5699:442-449.
17. Lee J.-Y., Li J., Yeung E.S. Anal. Chem. 2007, 79:8083-8089.
18. Li L., Wang X., Zhang X., Wang J., Jin W. Anal. Chim. Acta 2015, 854:122-128.
19. Linder V., Sia S.K., Whitesides G.M. Anal. Chem. 2005, 77:64-71.
20. Marinov G.K., Williams B.A., McCue K., Schroth G.P., Gertz J., Myers R.M., Wold B.J. Genome Res. 2014, 24:496-510.
21. Ottesen E.A., Hong J.W., Quake S.R., Leadbetter J.R. Science 2006, 314:1464-1467.
22. Perocchi F., Xu Z., Clauder-Münster S., Steinmetz L.M. Nucleic Acids Res. 2007, 35:e128.
23. Roy S.W., Irimia M. BioEssays 2008, 30:601-605.
24. Sahdev S., Saini S., Tiwari P., Saxena S., Singh Saini K. Mol. Cell. Probes 2007, 21:303-307.
25. Saiki R.K., Scharf S., Faloona F., Mullis K.B., Horn G.T., Erlich H.A., Arnheim N. Science 1985, 230:1350-1354.
26. Schmidt T., Schütz G.J., Baumgartner W., Gruber H.J., Schindler H. Proc. Natl. Acad. Sci. USA 1996, 93:2926-2929.
27. Schrader C., Schielke A., Ellerbroek L., Johne R. J. Appl. Microbiol. 2012, 113:1014-1026.
28. Ståhlberg A., Håkansson J., Xian X., Semb H., Kubista M. Clin. Chem. 2004, 50:509-515.
29. Ståhlberg A., Kubista M., Pfaffl M. Clin. Chem. 2004, 50:1678-1680.
30. Unger M.A., Chou H.-P., Thorsen T., Scherer A., Quake S.R. Science 2000, 288:113-116.
31. Wei C.-W., Cheng J.-Y., Huang C.-T., Yen M.-H., Young T.-H. Nucleic Acids Res. 2005, 33:e78.
32. Zhang H., Xu T., Li C.-W., Yang M. Biosens. Bioelectron. 2010, 25:2402-2407.
33. Zhong J.F., Chen Y., Marcus J.S., Scherer A., Quake S.R., Taylor C.R., Weiner L.P. Lab. Chip 2008, 8:68-74.