Epigenomic regulation of Smad1 signaling during cellular senescence induced by Ras activation

Methods in Molecular Biology

Volumn 1344, Issue , 2016, Pages 341-353

  Kaneda, Atsushi ^a   Nonaka, Aya ^a   Fujita, Takanori ^a   Yamanaka, Ryota ^a   Fujimoto, Mai ^a   Miyazono, Kohei ^a   Aburatani, Hiroyuki ^a  

^a NONE

Author keywords

Bmp2; Chromatin immunoprecipitation (ChIP); Epigenetics; Histone; Smad1

Indexed keywords

BETA GALACTOSIDASE; BONE MORPHOGENETIC PROTEIN 2; BUFFER; COMPLEMENTARY DNA; RAS PROTEIN; RETROVIRUS VECTOR; SMAD1 PROTEIN; HISTONE;

ANIMAL CELL; ANIMAL TISSUE; CELL AGING; CHROMATIN IMMUNOPRECIPITATION; CROSS LINKING; EMBRYO; EPIGENETICS; FEMALE; FIBROBLAST; GENE EXPRESSION; GENETIC ASSOCIATION; MOUSE; NONHUMAN; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RETROVIRIDAE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; ANIMAL; GENE EXPRESSION REGULATION; GENETIC EPIGENESIS; GENETICS; HIGH THROUGHPUT SEQUENCING; METABOLISM; PHYSIOLOGY; PROCEDURES; REPORTER GENE; TRANSCRIPTION INITIATION; TRANSFORMED CELL LINE;

ANIMALS; CELL AGING; CELL LINE, TRANSFORMED; CHROMATIN IMMUNOPRECIPITATION; EPIGENESIS, GENETIC; EPIGENOMICS; FIBROBLASTS; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENES, REPORTER; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HISTONES; MICE; RAS PROTEINS; REAL-TIME POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; SMAD1 PROTEIN; TRANSCRIPTIONAL ACTIVATION;

EID: 84946594314     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2966-5_22     Document Type: Chapter

References (15)

1. Feinberg AP (2007) Phenotypic plasticity and the epigenetics of human disease. Nature 447:433–440
2. Bracken AP, Kleine-Kohlbrecher D, Dietrich N, Pasini D, Gargiulo G et al (2007) The Polycomb group proteins bind throughout the INK4A-ARF locus and are disassociated in senescent cells. Genes Dev 21:525–530
3. Di Micco R, Sulli G, Dobreva M, Liontos M, Botrugno OA et al (2011) Interplay between oncogene-induced DNA damage response and heterochromatin in senescence and cancer. Nat Cell Biol 13:292–302
4. Hayflick L (1965) The limited in vitro lifetime of human diploid cell strains. Exp Cell Res 37:614–636
5. Kuilman T, Michaloglou C, Mooi WJ, Peeper DS (2010) The essence of senescence. Genes Dev 24:2463–2479
6. Serrano M, Lin AW, McCurrach ME, Beach D, Lowe SW (1997) Oncogenic ras provokes premature cell senescence associated with accumulation of p53 and p16INK4a. Cell 88: 593–602
7. Sharpless NE, De Pinho RA (2005) Cancer: crime and punishment. Nature 436:636–637
8. Campisi J (2005) Senescent cells, tumor suppression, and organismal aging: good citizens, bad neighbors. Cell 120:513–522
9. Prieur A, Peeper DS (2008) Cellular senescence in vivo: a barrier to tumorigenesis. Curr Opin Cell Biol 20:150–155
10. Narita M, Lowe SW (2005) Senescence comes of age. Nat Med 11:920–922
11. Kaneda A, Fujita T, Anai M, Yamamoto S, Nagae G et al (2011) Activation of Bmp2- Smad1 signal and its regulation by coordinated alteration of H3K27 trimethylation in Rasinduced senescence. PLoS Genet 7:e1002359
12. Kaneda A, Wang CJ, Cheong R, Timp W, Onyango P et al (2007) Enhanced sensitivity to IGF-II signaling links loss of imprinting of IGF2 to increased cell proliferation and tumor risk. Proc Natl Acad Sci U S A 104: 20926–20931
13. Kitamura T, Onishi M, Kinoshita S, Shibuya A, Miyajima A et al (1995) Efficient screening of retroviral cDNA expression libraries. Proc Natl Acad Sci U S A 92:9146–9150
14. Dimri GP, Lee X, Basile G, Acosta M, Scott G et al (1995) A biomarker that identifies senescent human cells in culture and in aging skin in vivo. Proc Natl Acad Sci U S A 92: 9363–9367
15. Li H, Handsaker B, Wysoker A, Fennell T, Ruan J et al (2009) The sequence alignment/ map format and SAMtools. Bioinformatics 25:2078–2079