Partitioning in Aqueous Two-Phase Systems: Fundamentals, Applications and Trends

Separation and Purification Reviews

Volumn 45, Issue 1, 2016, Pages 68-80

  Grilo, António Lima ^a   Aires Barros, M Raquel ^a   Azevedo, Ana M ^a  

^a INSTITUTO SUPERIOR TÉCNICO   (Portugal)

Author keywords

Aqueous two phase systems; liquid liquid equilibrium; monoclonal antibodies; purification; solvent extraction

Indexed keywords

ANTIBODIES; METAL IONS; METALS; MOLECULAR WEIGHT; MONOCLONAL ANTIBODIES; PURIFICATION;

AQUEOUS PHASIS; AQUEOUS TWO PHASE SYSTEM; DOWNSTREAM-PROCESSING; ELECTRICAL CHARGES; LIQUID LIQUID EQUILIBRIUM; LOW MOLECULAR WEIGHT; MAIN EFFECT; SETTLING TIME;

SOLVENT EXTRACTION;

EID: 84945903068     PISSN: 15422119     EISSN: 15422127     Source Type: Journal    
DOI: 10.1080/15422119.2014.983128     Document Type: Article

References (117)

1. Van Berlo, M., Luyben, K.C., and van der Wielen, L.A. (1998) Poly(ethylene glycol)-salt aqueous two-phase systems with easily recyclable volatile salts. J. Chrom. B, 711: 61-68.
2. Albertsson, P.-Å. (1985) History of aqueous polymer two-phase partition. In Partitioning in Aqueous Two-Phase System: Theory, Methods, Uses, and Applications to Biotechnology; Walter H, Brooks DE, Fisher D, eds. Academic Press: Orlando, Florida, 1-9.
3. Zaslavsky, B.Y. (1995) Aqueous Two-Phase Partitioning; Marcel Dekker: New York.
4. Johansson, G. (1994) Partitioning procedures and techniques: Small molecules and macromolecules. Meth. Enzym., 228: 28-42.
5. Albertsson, P.-Å. and Tjerneld, F. (1994) Phase diagrams. Meth. Enzym. 228: 3-13.
6. Nascimento, K.S., Yelo, S., Cavada, B.S., Azevedo, A.M., and Aires-Barros, M.R. (2011) Liquid-liquid equilibrium data for aqueous two-phase systems composed of ethylene oxide propylene oxide copolymers. J. Chem. Eng. Data, 56: 190-194.
7. Gu, T. (2000) Liquid-Liquid partitioning methods for bioseparations. In: Ahuja S, editor. Handbook of Bioseparations, 2nd ed.; Academic Press: San Diego, California, 329-364.
8. Cabral, J.M.S. (2007) Cell partitioning in aqueous two-phase polymer systems. Adv. Biochem. Eng. Biotech., 106: 151-171.
9. González-González, M. and Rito-Palomares, M. (2013) Aqueous twophase systems strategies to establish novel bioprocesses for stem cells recovery. Crit. Rev. Biotechnol., 85: 1-10.
10. Johansson, H.O., Karlström, G., and Tjerneld, F. (1997) Temperatureinduced phase partitioning of peptides in water solutions of ethylene oxide and propylene oxide random copolymers. Biochim. Biophys. Acta, 1335: 315-325.
11. Baskir, J.N., Hatton, T.A., and Suter, U.W. (1989) Protein partitioning in two-phase aqueous polymer systems. Biotech. Bioeng. 34: 541-558.
12. Hartounian, H., Kaler, E.W., and Sandler, S.I. (1994) Aqueous twophase systems. 2. Protein partitioning. Ind. Eng. Chem. Res., 33: 2294-2300.
13. Andrews, B.A., Nielsen, S., and Asenjo, J.A. (1996) Partitioning and purification of monoclonal antibodies in aqueous two-phase systems. Bioseparation, 6: 303-313.
14. Azevedo, A.M., Rosa, P.A.J., Ferreira, I.F., and Aires-Barros, M.R. (2009) Chromatography-free recovery of biopharmaceuticals through aqueous two-phase processing. Trends Biotechnol., 27: 240-247.
15. Alred, P.A., Kozlowski, A, Harris, J.M., and Tjerneld, F. (1994) Application of temperature-induced phase partitioning at ambient temperature for enzyme purification. J. Chrom. A, 659: 289-298.
16. Venâncio, A., Almeida, C., and Teixeira, J. (1996) Enzyme purification with aqueous two-phase systems: comparison between systems composed of pure polymers and systems composed of crude polymers. J. Chrom. B., 680: 131-136.
17. Pereira, J.F.B., Vicente, F., Santos-Ebinuma, V.C., Araújo, J.M., Pessoa, A., Freire, M.G., and Coutinho, J.A.P. (2013) Extraction of tetracycline from fermentation broth using aqueous two-phase systems composed of polyethylene glycol and cholinium-based salts. Proc. Biochem., 48: 716-722.
18. Sinha, J., Dey, P.K., and Panda, T. (2000) Aqueous two-phase: the system of choice for extractive fermentation. Appl. Microbiol. Biot., 54: 476-486.
19. Chen, J.-P. and Wang, C.-H. (1991) Lactose hydrolysis by b-Galactosidase in aqueous two-phase systems. J. Ferm. Bioeng., 71: 168-175.
20. Raja, S., Murty, V.R., Thivaharan, V., Rajasekar, V., and Ramesh, V. (2012) Aqueous two phase systems for the recovery of biomolecules-A review. Sci. Technol., 1: 7-16.
21. Rosa, P.A.J., Azevedo, A.M., Ferreira, I.F., de Vries, J., Korporaal, R., Verhoef, H.J., Visser, T.J., and Aires-Barros, M.R. (2007) Affinity partitioning of human antibodies in aqueous two-phase systems. J. Chrom. A, 1162: 103-113.
22. Rosa, P.A.J., Ferreira, I.F., Azevedo, A.M., and Aires-Barros, M.R. (2010) Aqueous two-phase systems: A viable platform in the manufacturing of biopharmaceuticals. J. Chrom. A, 1217: 296-305.
23. Johansson, G. (1978) Comparison of two aqueous biphasic systems used for the partition of biological material. J. Chrom., 150: 63-71.
24. Mehrnoush, A., Mustafa, S., Sarker, M.Z.I., and Yazid, A.M.M. (2012) Optimization of serine protease purification from mango (Mangifera indica cv. Chokanan) peel in polyethylene glycol/dextran aqueous two phase system. Int. J. Mol. Sci., 13: 3636-3649.
25. Azevedo, A.M., Rosa, P.A.J., Ferreira, I.F., de Vries, J., Visser, T.J., and Aires-Barros, M.R. (2009) Downstream processing of human antibodies integrating an extraction capture step and cation exchange chromatography. J. Chrom. B, 877: 50-58.
26. Zijlstra, G.M., Michielsen, M.J., de Gooijer, C.D., van der Pol, L.A., and Tramper, J. (1996) Hybridoma and CHO cell partitioning in aqueous two-phase systems. Biotechnol. Prog., 12: 363-370.
27. Oliveira, L., Sarubbo, L., Porto, A.L., Campos-Takaki, G., and Tambourgi, E. (2002) Partition of trypsin in aqueous two-phase systems of poly(ethylene glycol) and cashew-nut tree gum. Proc. Biochem., 38: 693-699.
28. Sarubbo, L.A., Oliveira, L.A., Porto, A.L., Duarte, H.S., Carneiro-Leão, A.M., Lima-Filho, J.L., Campos-Takakid, G.M., and Tambourgi, E.B. (2000) New aqueous two-phase system based on cashew-nut tree gum and poly(ethylene glycol). J. Chrom. B, 743: 79-84.
29. Da Silva, L.H.M. and Meirelles, A.J.A. (2001) Phase equilibrium and protein partitioning in aqueous mixtures of maltodextrin with polypropylene glycol. Carbohy. Polym., 46: 267-274.
30. Ferreira, I.F., Azevedo, A.M., Rosa, P.A.J., and Aires-Barros, M.R. (2008) Purification of human immunoglobulin G by thermoseparating aqueous two-phase systems. J. Chrom. A, 1195: 94-100.
31. Van Alstine, J., Shanagar, J., Hjorth, R., Hall, M., Estmer, N.C. (2010) Separation Method Using Single Polymer Phase Systems. European Patent EP2373676 A1, July 15, 2010.
32. Ng, H.S., Tan, C.P., Mokhtar, M.N., Ibrahim, S., Ariff, A., Ooi, C.W., and Ling, T.C. (2012) Recovery of Bacillus cereus cyclodextrin glycosyltransferase and recycling of phase components in an aqueous two-phase system using thermo-separating polymer. Sep. Purif. Tech., 89: 9-15.
33. Ruiz-Ruiz, F., Benavides, J., Aguilar, O., and Rito-Palomares, M. (2012) Aqueous two-phase affinity partitioning systems: current applications and trends. J. Chrom. A, 1244: 1-13.
34. Tubio, G., Picó, G.A., and Nerli, B.B. (2009) Extraction of trypsin from bovine pancreas by applying polyethyleneglycol/sodium citrate aqueous two-phase systems. J. Chrom. B, 877: 115-120.
35. Azevedo, A.M., Gomes, A.G., Rosa, P.A.J., Ferreira, I.F., Pisco, A.M.M.O., and Aires-Barros, M.R. (2009) Partitioning of human antibodies in polyethylene glycol-sodium citrate aqueous two-phase systems. Sep. Purif. Tech., 65: 14-21.
36. Tubío, G., Nerli, B., and Picó, G. (2007) Partitioning features of bovine trypsin and alpha-chymotrypsin in polyethyleneglycol-sodium citrate aqueous two-phase systems. J. Chrom. B, 852: 244-249.
37. Iyyaswami, R., Belur, P.D., Girish, B., and Nagaraj, V.H. (2012) Development and Evaluation of PEG-Lithium Citrate Salt Based Aqueous Two Phase System and Its Application in Partitioning of Proteins from Fish Industry Effluent. Sep. Sci. Tech., 47: 591-598.
38. Gomes, G.A., Azevedo, A.M., Aires-Barros, M.R., and Prazeres, D.M.F. (2009) Purification of plasmid DNA with aqueous two phase systems of PEG 600 and sodium citrate/ammonium sulfate. Sep. Purif. Tech., 65: 22-30.
39. Azevedo, A.M., Rosa, P.A.J., Ferreira, I.F., and Aires-Barros, M.R. (2008) Integrated process for the purification of antibodies combining aqueous two-phase extraction, hydrophobic interaction chromatography and size-exclusion chromatography. J. Chrom. A, 1213: 154-161.
40. Ivetic, D.Z., Sciban, M.B., Vasic, V.M., Kukic, D.V., Prodanovic, J.M., and Antov, M.G. (2013) Evaluation of possibility of textile dye removal from wastewater by aqueous two-phase extraction. Desal. Water Treat., 51: 1603-1608.
41. Ferreira, J.F., Bresolin, I.R.P., Silveira, E., and Tambourgi, E.B. (2011) Purification of bromelain from Ananas comosus by PEG/phosphate ATPS. Chem. Eng. Trans., 24: 931-936.
42. Pei, Y., Li, Z., Liu, L., and Wang, J. (2012) Partitioning behavior of amino acids in aqueous two-phase systems formed by imidazolium ionic liquid and dipotassium hydrogen phosphate. J. Chrom. A, 1231: 2-7.
43. Selvakumar, P., Ling, T.C., Walker, S., and Lyddiatt, A. (2012) Recovery of glyceraldehyde 3-phosphate dehydrogenase from an unclarified disrupted yeast using aqueous two-phase systems facilitated by distribution analysis of radiolabelled analytes. Sep. Purif. Tech., 85: 28-34.
44. De Souza, R.L., Barbosa, J.M.P., Zanin, G.M., Lobão, M.W.N., Soares, C.M.F., and Lima, A.S. (2010) Partitioning of porcine pancreatic lipase in a two-phase systems of polyethylene glycol/potassium phosphate aqueous. Appl. Biochem. Biotech., 161: 288-300.
45. Bim, M.A. and Franco, T.T. (2000) Extraction in aqueous two-phase systems of alkaline xylanase produced by Bacillus pumilus and its application in kraft pulp bleaching. J. Chrom. B, 743: 349-356.
46. Silva, C.S., Bovarotti, E., Rodrigues, M.I., Hokka, C.O., and Barboza, M. (2009) Evaluation of the effects of the parameters involved in the purification of clavulanic acid from fermentation broth by aqueous two-phase systems. Bioproc. Biosyst. Eng., 32: 625-632.
47. Rosa, P.A.J., Azevedo, A.M., Sommerfeld, S., Mutter, M., Bäcker, W., and Aires-Barros, M.R. (2012) Continuous purification of antibodies from cell culture supernatant with aqueous two-phase systems: from concept to process. Biotech., J 8: 352-362.
48. Azevedo, A.M., Rosa, P.A.J., Ferreira, I.F., and Aires-Barros, M.R. (2007) Optimisation of aqueous two-phase extraction of human antibodies. J. Biotech., 132: 209-217.
49. Benavides, J., Mena, J.A., Cisneros-Ruiz, M., Ramírez, O.T., Palomares, L.A., and Rito-Palomares, M. (2006) Rotavirus-like particles primary recovery from insect cells in aqueous two-phase systems. J. Chrom. B, 842: 48-57.
50. Antov, M.G., Peričin, D.M., and Trbojević, B.I. (2004) The effect of sulphates on partitioning of pectinases in aqueous two-phase systems. Acta Period. Tecnol., 2004: 179-186.
51. Zhu, J.-H., Wei, D.-Z., Cao, X.-J., Liu, Y.-Q., and Yuan, Z.-Y. (2001) Partitioning behaviour of cephalexin and 7-aminodeacetoxicephalosporanic acid in PEG/ammonium sulfate aqueous two-phase systems. J. Chem. Tech. Biotech., 76: 1194-1200.
52. Salabat, A., Abnosi, M.H., and Bahar, A.R. (2007) Amino acids partitioning in aqueous two-phase system of polypropylene glycol and magnesium sulfate. J. Chrom. B, 858: 234-238.
53. Mohamadi, H.S., Omidinia, E., and Dinarvand, R. (2007) Evaluation of recombinant phenylalanine dehydrogenase behavior in aqueous two-phase partitioning. Proc. Biochem., 42: 1296-1301.
54. Gu, Z. and Glatz, C.E. (2007) Aqueous two-phase extraction for protein recovery from corn extracts. J. Chrom. B, 845: 38-50.
55. Salabat, A., Far, M.R., and Moghadam, S.T. (2010) Partitioning of amino acids in surfactant based aqueous two-phase systems containing the nonionic surfactant (Triton X-100) and salts. J. Sol. Chem., 40: 61-66.
56. 4 and PEG1500 aqueous two-phase system. Phys. Chem. Liq., 48: 764-772.
57. Madhusudhan, M.C. and Raghavarao, K.S.M.S. (2011) Aqueous two phase extraction of invertase from baker's yeast: Effect of process parameters on partitioning. Proc. Biochem., 46: 2014-2020.
58. Cunha, M.T., Aires-Barros, R., and Cabral, J.M.S. (2003) Extraction for rapid protein isolation. In Isolation and Purification of Proteins; Kaul, R. and Mattiasson, B., eds.; Marcel Dekker: New York.
59. Johansson, H.-O., Svensson, M., Persson, J., and Tjerneld, F. (2001) Aqueous two-phase systems with smart polymers. In Smart Polymers: Applications in Biotechnology and Biomedicine; Galaev, I. and Mattiasson, B., eds. CRC Press: Boca Raton, Florida.
60. Hernandez-Mireles, T. and Rito-Palomares, M. (2006) New aqueous two-phase systems based on poly (ethylene oxide sulfide) (PEOS) and potassium phosphate for the potential recovery of proteins. J. Chem. Tech. Biotech., 81: 997-1002.
61. Simeone, M., Alfani, A., and Guido, S. (2004) Phase diagram, rheology and interfacial tension of aqueous mixtures of Na-caseinate and Na-alginate. Food Hydrocoll., 18: 463-470.
62. Kadir, S., Decloux, M., Giampaoli, P., and Joulia, X. (2008) Liquid-Liquid Equilibria of the Ternary Systems 3-Methyl-1-butanol + Ethanol + Water and 2-Methyl-1-propanol + Ethanol + Water at 293.15 K. J. Chem. Eng. Data, 53: 910-912.
63. Wang, Y., Mao, Y., Han, J., Liu, Y., and Yan, Y. (2010) Liquid-liquid equilibrium of potassium phosphate/potassium citrate/sodium citrate + ethanol aqueous two-phase systems at (298.15 and 313.15) K and correlation. J. Chem. Eng. Data, 55: 5621-5626.
64. Saravanan, S., Rao, J.R., Nair, B.U., and Ramasami, T. (2008) Aqueous two-phase poly(ethylene glycol)-poly(acrylic acid) system for protein partitioning: Influence of molecular weight, pH and temperature. Proc. Biochem., 43: 905-911.
65. Henrique, L., Hespanhol, C., de Sousa, R.C.S., Martins, J.P., Rodrigues, G.D., Coimbra, J.S.R., and Minim, L.A. (2009) Surface excess enthalpy of PEO + salt + water and L35 + salt + water two-phase systems. J. Chem. Eng. Data, 54: 531-535.
66. Liu, Y., Wu, Z., and Dai, J. (2012) Phase equilibrium and protein partitioning in aqueous micellar two-phase system composed of surfactant and polymer. Fluid Phase Equilibr., 320: 60-64.
67. Liu, Y., Wu, Z., Zhang, Y., and Yuan, H. (2012) Partitioning of biomolecules in aqueous two-phase systems of polyethylene glycol and nonionic surfactant. Biochem. Eng. J., 69: 93-99.
68. Deive, F.J., Rivas, M.A., and Rodríguez, A. (2011) Sodium carbonate as phase promoter in aqueous solutions of imidazolium and pyridinium ionic liquids. J. Chem. Therm., 43: 1153-1158.
69. Yu, C., Han, J., Wang, Y., Yan, Y., Hu, S., Li, Y., and Zhao, X. (2011) Liquid-liquid equilibrium composed of imidazolium tetrafluoroborate ionic liquids+sodium carbonate aqueous two-phase systems and correlation (at 288.15, 298.15, and 308.15 K). Thermochim. Acta, 523: 221-226.
70. Pei, Y., Li, L., Li, Z., Wu, C., Wang, J. (2012) Partitioning behavior of wastewater proteins in some ionic liquids-based aqueous two-phase systems. Sep. Sci. Tech., 47: 277-283.
71. Passos, H., Ferreira, A.R., Cláudio, A.F.M., Coutinho, J.a.P., and Freire, M.G. (2012) Characterization of aqueous biphasic systems composed of ionic liquids and a citrate-based biodegradable salt. Biochem. Eng. J., 67: 68-76.
72. Bolognese, B., Nerli, B., and Picó, G. (2005) Application of the aqueous two-phase systems of ethylene and propylene oxide copolymer-maltodextrin for protein purification. J. Chrom. B, 814: 347-53.
73. Albertsson, P.-Å. (1960) Partition of Cell Particles and Macromolecules; Uppsala University: Uppsala, Swede.
74. Albertsson, P.-Å. (1995) Aqueous biphasic systems: properties and applications in bioseparation. In Aqueous Biphasic Separations: Biomolecules to Metal Ions, 1st ed.; Rogers, R.D. and Eiteman, M.A., eds. Plenum: New York, 21-30.
75. Banik, R.M., Santhiagu, A., and Kanari, B. (2003) Technological aspects of extractive fermentation using aqueous two-phase systems. World J. Microb. Biotech., 19: 337-348.
76. Cabezas, H. (1996) Theory of phase formation in aqueous two-phase systems. J. Chrom. B, 680: 3-30.
77. Cabezas, H., Evans, J.D., and Szlag, D.C. (1990) Statistical thermodynamics of aqueous two-phase systems. In Downstream Processing and Bioseparation; Hamel, J.-F.P., Hunter, J.B. and Sikdar, S.K., eds.; American Chemical Society: New York, 38-52.
78. King, R.S., Blanch, H.W., and Prausnitz, J.M. (1988) Molecular thermodynamics of aqueous two-phase systems for bioseparations. AIChE J., 34: 1585-1594.
79. Baskir, J.N., Hatton, T.A., and Suter, U.W. (1987) Thermodynamics of the separation of biomaterials in two-phase aqueous polymer systems: Effect of the phase-forming polymers. Macromolecules, 20: 1300-1311.
80. Van der Shoot, P.P.A. and Leermakers, F.A.M. (1988) Comment on "Thermodynamics of the separation of biomaterials in two-phase aqueous polymer systems: Effect of the phase-forming polymers." Macromolecules, 21: 1876-1877.
81. Baskir, J.N., Hatton, T.A., and Suter, U.W. (1989) Thermodynamics of the partitioning of biomaterials in two-phase aqueous polymer systems: Comparison of lattice model to experimental data. J. Phys. Chem., 93: 2111-2122.
82. Diamond, A.D. and Hsu, J.T. (1990) Correlation of protein partitioning in aqueous polymer two-phase systems. J. Chrom. A, 513: 137-143.
83. Guzmán, R. and Téllez, C.M. (1995) Affinity partitioning of metal ions in aqueous biphasic systems: experimental and theoretical aspects. In Aqueous Biphasic Separations: Biomolecules to Metal Ions, 1st ed.; Rogers, R.D. and Eiteman, M.A., eds.; Plenum: New York, 101-117.
84. Madeira, P.P., Reis, C.A., Rodrigues, A.E., Mikheeva, L.M., and Zaslavsky, B.Y. (2010) Solvent properties governing solute partitioning in polymer/polymer aqueous two-phase systems: nonionic compounds. J. Phys. Chem. B, 114: 457-62.
85. Madeira, P.P., Reis, C.A., Rodrigues, A.E., Mikheeva, L.M., Chait, A., and Zaslavsky, B.Y. (2011) Solvent properties governing protein partitioning in polymer/polymer aqueous two-phase systems. J. Chromatogr. A, 1218: 1379-1384.
86. Madeira, P.P., Bessa, A., Alvares-Ribeiro, L., Aires-Barros, M.R., Reis, C.A., Rodrigues, A.E., and Zaslavsky, B.Y. (2012) Salt effects on solvent features of coexisting phases in aqueous polymer/polymer two-phase systems. J. Chrom. A, 1229: 38-47.
87. Wu, Y.-T., Lin, D.-Q., and Zhu, Z.-Q. (1998) Thermodynamics of aqueous two-phase systems-the effect of polymer molecular weight on liquid-liquid equilibrium phase diagrams by the modified NRTL model. Fluid Phase Equilibr., 147: 25-43.
88. Perez, B., Malpiedi, L.P., Tubío, G., Nerli, B., and de Alcântara Pessôa Filho, P. (2013) Experimental determination and thermodynamic modeling of phase equilibrium and protein partitioning in aqueous two-phase systems containing biodegradable salts. J. Chem. Therm., 56: 136-143.
89. Luechau, F., Ling, T.C., and Lyddiatt, A. (2009) Selective partition of plasmid DNA and RNA in aqueous two-phase systems by the addition of neutral salt. Sep. Purif. Technol., 68: 114-118.
90. Andrews, B.A. and Asenjo, J.A. (2010) Theoretical and experimental evaluation of hydrophobicity of proteins to predict their partitioning behavior in aqueous two phase systems: A review. Sep. Sci. Technol., 45: 2165-2170.
91. Rocha, M.V. and Nerli, B.B. (2013) Molecular features determining different partitioning patterns of papain and bromelain in aqueous two-phase systems. Int. J. Biol. Macromol., 61: 204-211.
92. Ratanapongleka, K. (2010) Recovery of biological products in aqueous two phase systems. Int. J. Chem. Eng. Appl., 1: 191-198.
93. Fernández-Tornero, C., García, E., López, R., Giménez-Gallego, G., and Romero, A. (2002) Two new crystal forms of the cholinebinding domain of the major pneumococcal autolysin: Insights into the dynamics of the active homodimer. J. Mol. Biol., 321: 163-173.
94. Johansson, G. (1974) Effects of salts on the partition of proteins in aqueous polymeric biphasic systems. Acta Chem. Scand. Ser. B, 28: 873-82.
95. Cunha, M.T., Tjerneld, F., Cabral, J.M.S., and Aires-Barros, M.R. (1998) Effect of electrolytes and surfactants on the thermoseparation of an ethylene oxide-propylene oxide random copolymer in aqueous solution. J. Chrom. B, 711: 53-60.
96. Sharma, R. and Bahadur, P. (2002) Effect of different additives on the cloud point of a polyethylene oxide-polypropylene oxidepolyethylene oxide block copolymer in aqueous solution. J. Surfact. Deterg., 5: 263-268.
97. Salabat, A. (2001) The influence of salts on the phase composition in aqueous two-phase systems: experiments and predictions. Fluid Phase Equilibr., 187-188:489-498.
98. Bamberger, S., Seaman, G.V., Sharp, K., and Brooks, D.E. (1984) The effects of salts on the interfacial tension of aqueous dextran poly(ethylene glycol) phase systems. J. Coll. Interf. Sci., 99: 194-200.
99. Forciniti, D., Hall, C.K., and Kula, M.R. (1990) Interfacial tension of polyethyleneglycol-dextran-water systems: Influence of temperature and polymer molecular weight. J. Biotechnol., 16: 279-296.
100. Wu, Y.-T. and Zhu, Z.-Q. (1999) Modeling of interfacial tension of aqueous two-phase systems. Chem. Eng. Sci., 54: 433-440.
101. Asenjo, J.A., Mistry, S.L., Andrews, B.A., and Merchuk, J.C. (2002) Phase separation rates of aqueous two-phase systems: correlation with system properties. Biotech. Bioeng., 79: 217-223.
102. Ding, P., Wolf, B., Frith, W.J., Clark, A.H., Norton, I.T., and Pacek, A.W. (2002) Interfacial tension in phase-separated gelatin/dextran aqueous mixtures. J. Coll. Interf. Sci., 253: 367-376.
103. Ding, P., Pacek, A., Frith, W., Norton, I., and Wolf, B. (2005) The effect of temperature and composition on the interfacial tension and rheology of separated phases in gelatin/pullulan mixtures. Food Hydrocoll., 19: 567-574.
104. Liu, Y., Lipowsky, R., and Dimova, R. (2012) Concentration dependence of the interfacial tension for aqueous two-phase polymer solutions of dextran and polyethylene glycol. Langmuir, 28: 3831-3839.
105. Barnea, E. and Mizrahi, J. (1975) Separation mechanism of liquidliquid dispersions in a deep-layer gravity settler: Part IV - Continuous settler characteristics. Trans. Inst. Chem. Eng., 56: 83-92.
106. Mei, L., Lin, D.-Q., Zhu, Z.-Q., and Han, Z.-X. (1995) Densities and viscosities of polyethylene glycol + salt + water systems at 20°C. J. Chem. Eng. Data, 40: 1168-1171.
107. Rito-Palomares, M. and Cueto, L. (2000) Effect of biological suspensions on the position of the binodal curve in aqueous two-phase systems. J. Chrom. B, 743: 5-12.
108. Solano-Castillo, C. and Rito-Palomares, M. (2000) Kinetics of phase separation under different process and design parameters in aqueous two-phase systems. J. Chrom. B, 743: 195-201.
109. Ahmad, M.M. and Przybycien, T.M. (2013) Towards optimal aqueous two-phase extraction system flowsheets for protein purification. J. Chem. Technol. Biotechnol., 88: 62-71.
110. Espitia-Saloma, E., Vázquez-Villegas, P., Aguilar, O., and Rito-Palomares, M. (2014) Continuous aqueous two-phase systems devices for the recovery of biological products. Food Bioprod. Proc., 92: 101-112.
111. Vázquez-Villegas, P., Espitia-Saloma, E., Rito-Palomares, M., and Aguilar, O. (2013) Low-abundant protein extraction from complex protein sample using a novel continuous aqueous two-phase systems device. J. Sep. Sci., 36: 391-399.
112. Cramer, S., Sheth, R., Mi, J., and Bhut, B. (2013) Smart biopolymer affinity precipitation for mAb purification: Methods development and process considerations. 17th BioPartitioning Purification Conference, Presentation O-301, October 6-10, Newport, Rhode Island, USA.
113. Piper, R., Vedantham, G., Grilo, A.L., Lacki, K., and van Alstine, J. (2013) Investigation of alternative separation technologies for the harvest of CHO cell culture. 17th BioPartitioning Purification Conference, Presentation O-101, October 6-10, Newport, Rhode Island, USA.
114. Lee, J.-W. and Forciniti, D. (2013) Effect of glycosylation on the partition behavior of a human antibody in aqueous two-phase systems. Biotechnol. Prog., 29: 943-950.
115. Madeira, P.P., Bessa, A., Álvares-Ribeiro, L., Aires-Barros, M.R., Rodrigues, A.E., and Zaslavsky, B.Y. (2013) Analysis of amino acidwater interactions by partitioning in aqueous two-phase systems. I-Amino acids with non-polar side-chains. J. Chromatogr. A, 1274: 82-86.
116. Nagel, S., Gretzinger, S., Schwab, M., Oelmeier, S.A., Dismer, F., and Hubbuch, J. (2013) Aqueous two-phase systems-A label-free technology for cell separation. Cytotherapy, 15: 45-58.
117. Zaslavsky, B.Y. (1992) Bioanalytical applications of partitioning in aqueous polymer two-phase systems. Anal. Chem., 64: 765A-773A.