Adult lung spheroid cells contain progenitor cells and mediate regeneration in rodents with bleomycin-induced pulmonary fibrosis

Stem Cells Translational Medicine

Volumn 4, Issue 11, 2015, Pages 1265-1274

  Henry, Eric ^a,b   Cores, Jhon ^a,b   Hensley, M Taylor ^a   Anthony, Shirena ^a   Vandergriff, Adam ^a,b   De Andrade, James B M ^a   Allen, Tyler ^a   Caranasos, Thomas G ^c   Lobo, Leonard J ^d   Cheng, Ke ^a,b  

^a NORTH CAROLINA STATE UNIVERSITY   (United States)

^b North Carolina 27599 and North Carolina State University   (United States)

^c UNIVERSITY OF NORTH CAROLINA   (United States)

^d UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

Author keywords

Adult lung stem cells; Mesenchymal stem cells; Pulmonary fibrosis; Spheroid cell culture

Indexed keywords

AQUAPORIN 5; BONE MORPHOGENETIC PROTEIN 2; CHEMOKINE RECEPTOR CXCR4; FIBROBLAST GROWTH FACTOR 2; ANTINEOPLASTIC ANTIBIOTIC; BLEOMYCIN;

ANGIOGENESIS; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; APOPTOSIS; ARTICLE; BLEOMYCIN-INDUCED PULMONARY FIBROSIS; CELL DIFFERENTIATION; CELL INFILTRATION; FEMALE; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; MOUSE; NONHUMAN; POLYMERASE CHAIN REACTION; RAT; SPHEROID CELL; STEM CELL NICHE; TISSUE REGENERATION; ADULT; ANIMAL; CHEMICALLY INDUCED; MALE; MESENCHYMAL STEM CELL TRANSPLANTATION; MESENCHYMAL STROMA CELL; METABOLISM; MULTICELLULAR SPHEROID; PATHOLOGY; PULMONARY FIBROSIS; REGENERATION; SCID MOUSE; TRANSPLANTATION; XENOGRAFT;

ADULT; ANIMALS; ANTIBIOTICS, ANTINEOPLASTIC; BLEOMYCIN; FEMALE; HETEROGRAFTS; HUMANS; MALE; MESENCHYMAL STEM CELL TRANSPLANTATION; MESENCHYMAL STROMAL CELLS; MICE; MICE, SCID; PULMONARY FIBROSIS; REGENERATION; SPHEROIDS, CELLULAR;

EID: 84945262406     PISSN: 21576564     EISSN: 21576580     Source Type: Journal    
DOI: 10.5966/sctm.2015-0062     Document Type: Article

References (28)

1. Cottin V. Interstitial lung disease. Eur Respir Rev 2013;22:26-32.
2. Yang J, Jia Z. Cell-based therapy in lung regenerative medicine. Regen Med Res 2014;2:7.
3. Takahashi K, Tanabe K, OhnukiMet al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 2007; 131:861-872.
4. Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 2006;126:663-676.
5. Thomson JA, Itskovitz-Eldor J, Shapiro SS et al. Embryonic stem cell lines derived from human blastocysts. Science 1998;282:1145-1147.
6. Moodley Y, Atienza D, Manuelpillai Uet al. Human umbilical cord mesenchymal stem cells reduce fibrosis of bleomycin-induced lung injury. Am J Pathol 2009;175:303-313.
7. Ortiz LA, Dutreil M, Fattman C et al. Interleukin 1 receptor antagonist mediates the antiinflammatory and antifibrotic effect of mesenchymal stem cells during lung injury. Proc Natl Acad Sci USA 2007;104:11002-11007.
8. Ortiz LA, Gambelli F, McBride C et al. Mesenchymal stem cell engraftment in lung is enhanced in response to bleomycin exposure and ameliorates its fibrotic effects. Proc Natl Acad Sci USA 2003;100:8407-8411.
9. Tzouvelekis A, Paspaliaris V, Koliakos G et al. A prospective, non-randomized, no placebo-controlled, phase Ib clinical trial to study the safety of the adipose derived stromal cells-stromal vascular fraction in idiopathic pulmonary fibrosis. J Transl Med 2013;11:171.
10. Desai TJ, Brownfield DG, KrasnowMA.Alveolarprogenitorandstemcells in lungdevelopment, renewal and cancer. Nature 2014;507:190-194.
11. Kajstura J, Rota M, Hall SR et al. Evidence for human lung stem cells. N Engl J Med 2011; 364:1795-1806.
12. Kim CF, Jackson EL, Woolfenden AE et al. Identification of bronchioalveolar stemcells in normal lung and lung cancer. Cell 2005;121:823-835.
13. Wansleeben C, Barkauskas CE, Rock JR et al. Stemcellsof theadult lung: Theirdevelopmentand role in homeostasis, regeneration, and disease. Wiley Interdiscip Rev Dev Biol 2013;2:131-148.
14. Barkauskas CE, Cronce MJ, Rackley CR et al. Type 2 alveolar cells are stem cells in adult lung. J Clin Invest 2013;123:3025-3036.
15. Hogan BL, Barkauskas CE, Chapman HA et al. Repair and regeneration of the respiratory system: Complexity, plasticity, and mechanisms of lung stem cell function. Cell Stem Cell 2014; 15:123-138.
16. Fennema E, Rivron N, Rouwkema J et al. Spheroid cultureasa tool for creating 3Dcomplex tissues. Trends Biotechnol 2013;31:108-115.
17. LaBarbera DV, Reid BG, Yoo BH. The multicellular tumor spheroid model for highthroughput cancer drug discovery. Expert Opin Drug Discov 2012;7:819-830.
18. Deleyrolle L, Reynolds B. Isolation, expansion, and differentiation of adult mammalian neural stem and progenitor cells using the neurosphere assay. In: Gordon D, Scolding NJ, eds. Neural Cell Transplantation. New York, NY: Humana Press, 2009:91-101.
19. Marbán E. Breakthroughs in cell therapy for heart disease: Focus on cardiospherederived cells. Mayo Clin Proc 2014;89:850-858.
20. Su G, Zhao Y, Wei J et al. The effect of forced growth of cells into 3D spheres using low attachmentsurfacesonthe acquisitionof stemness properties. Biomaterials 2013;34:3215-3222.
21. Su G, Zhao Y,Wei J et al. Direct conversion of fibroblasts into neural progenitor-like cells by forced growth into 3D spheres on low attachment surfaces. Biomaterials 2013;34:5897-5906.
22. LiTS,ChengK,MalliarasKetal.Directcomparison of different stemcell types and subpopulations reveals superior paracrine potency and myocardial repair efficacy with cardiospherederived cells. JAm Coll Cardiol 2012;59:942-953.
23. Li T-S,Cheng K, LeeS-T et al.Cardiospheres recapitulate a niche-like microenvironment rich instemness andcell-matrix interactions, rationalizing their enhanced functional potency formyocardial repair. Stem Cells 2010;28:2088-2098.
24. Kotton DN, Morrisey EE. Lung regeneration: Mechanisms, applications and emerging stemcell populations. NatMed 2014;20:822-832.
25. Makkar RR, Smith RR, Cheng K et al. Intracoronary cardiosphere-derived cells for heart regeneration after myocardial infarction (CADUCEUS): A prospective, randomised phase 1 trial. Lancet 2012;379:895-904.
26. AmannA,ZwierzinaM,GamerithGetal.Development of an innovative 3D cell culture system to study tumour-stroma interactions in non-small cell lung cancer cells. PLoS One 2014;9:e92511.
27. TataPR,MouH,Pardo-SagantaAet al.Dedifferentiation of committed epithelial cells into stem cells in vivo. Nature 2013;503:218-223.
28. Chimenti I, Smith RR, Li T-S et al. Relative roles of direct regeneration versus paracrine effects of human cardiosphere-derived cells transplanted into infarcted mice. Circ Res 2010;106:971-980.