Isolation, expansion, and differentiation of adult mammalian neural stem and progenitor cells using the neurosphere assay

Methods in Molecular Biology

Volumn 549, Issue , 2009, Pages 91-101

  Deleyrolle, Loic P ^a   Reynolds, Brent A ^b  

^a UNIVERSITY OF FLORIDA COLLEGE OF MEDICINE   (United States)

^b UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

Cell culture; Central nervous system; Neural stem cell; Neurospheres

Indexed keywords

MAMMALIA;

ANIMAL; BIOASSAY; CELL CULTURE; CELL DIFFERENTIATION; CELL LINEAGE; CULTURE TECHNIQUE; CYTOLOGY; IMMUNOHISTOCHEMISTRY; INSTRUMENTATION; METHODOLOGY; MULTICELLULAR SPHEROID; NERVE CELL; PHYSIOLOGY; REVIEW; STEM CELL;

ANIMALS; BIOLOGICAL ASSAY; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL LINEAGE; CELLS, CULTURED; IMMUNOHISTOCHEMISTRY; NEURONS; SPHEROIDS, CELLULAR; STEM CELLS;

EID: 67649337173     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60327-931-4_7     Document Type: Article

References (8)

1. Gross, C. G. (2000). Neurogenesis in the adult brain: death of a dogma. Nat Rev Neurosci 1, 67-73.
2. Reynolds, B. A., and Weiss, S. (1992). Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science 255, 1707-10.
3. Reynolds, B. A., and Weiss, S. (1996). Clonal and population analyses demonstrate that an EGF-responsive mammalian embryonic CNS precursor is a stem cell. Dev Biol 175, 1-13. (Pubitemid 26150042)
4. Potten, C. S., and Loeffler, M. (1990). Stem cells: attributes, cycles, spirals, pitfalls and uncertainties. Lessons for and from the crypt. Development 110, 1001-20. (Pubitemid 120009472)
5. Reynolds, B. A., and Rietze, R. L. (2005). Neural stem cells and neurospheres-re-evaluating the relationship. Nat Methods 2, 333-6. (Pubitemid 41130977)
6. Evans, G. S., and Potten, C. S. (1991) Stem cells and the elixir of life. Bioessays 13, 135-8. (Pubitemid 121000554)
7. Foroni, C., Galli, R., Cipelletti, B., Caumo, A., Alberti, S., Fiocco, R., and Vescovi, A. (2007). Resilience to transformation and inherent genetic and functional stability of adult neural stem cells ex vivo. Cancer Res 67, 3725-33. (Pubitemid 46762158)
8. Hall, P. A., and Watt, F. M. (1989). Stem cells: the generation and maintenance of cellular diversity. Development 106, 619-33. (Pubitemid 19221519)