Direct conversion of fibroblasts into neural progenitor-like cells by forced growth into 3D spheres on low attachment surfaces

Biomaterials

Volumn 34, Issue 24, 2013, Pages 5897-5906

  Su, Guannan ^a,b   Zhao, Yannan ^a   Wei, Jianshu ^a,b   Xiao, Zhifeng ^a   Chen, Bing ^a   Han, Jin ^a   Chen, Lei ^a,b   Guan, Jian ^c   Wang, Renzhi ^c   Dong, Qun ^d   Dai, Jianwu ^a  

^a INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c PEKING UNION MEDICAL COLLEGE HOSPITAL   (China)

^d St Joseph's Hospital   (United States)

Author keywords

3D sphere culture; Neural progenitor cells; Reprogramming; Transdifferentiation

Indexed keywords

CLOSE RELATIONSHIPS; DIRECT CONVERSION; EMBRYONIC STEM CELLS; MOUSE-FIBROBLASTS; NEURAL PROGENITOR CELLS; PHYSICAL APPROACHES; REPROGRAMMING; TRANS DIFFERENTIATIONS;

CELL CULTURE; FIBROBLASTS; MORPHOLOGY; STEM CELLS; THREE DIMENSIONAL COMPUTER GRAPHICS;

SPHERES;

TRANSCRIPTION FACTOR SOX2;

ANIMAL CELL; ARTICLE; ASTROCYTE; CELL DIFFERENTIATION; CELL GROWTH; CELL STRAIN HEK293; CELL STRUCTURE; CONTROLLED STUDY; FIBROBLAST; GENE OVEREXPRESSION; HIPPOCAMPUS; IN VITRO STUDY; NEURAL STEM CELL; NONHUMAN; NUCLEAR REPROGRAMMING; OLIGODENDROGLIA; PRIORITY JOURNAL; RAT;

ANIMALS; CELL ADHESION; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL PROLIFERATION; EMBRYO, MAMMALIAN; FIBROBLASTS; HEK293 CELLS; HUMANS; MALE; MICE; MICE, INBRED C57BL; MICE, INBRED ICR; NEURAL STEM CELLS; RATS, SPRAGUE-DAWLEY; SPHEROIDS, CELLULAR; SURFACE PROPERTIES; TAIL;

EID: 84878033541     PISSN: 01429612     EISSN: 18785905     Source Type: Journal    
DOI: 10.1016/j.biomaterials.2013.04.040     Document Type: Article

References (40)

1. Takahashi K., Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 2006, 126:663-676.
2. Kim D., Kim C.H., Moon J.I., Chung Y.G., Chang M.Y., Han B.S., et al. Generation of human induced pluripotent stem cells by direct delivery of reprogramming proteins. Cell Stem Cell 2009, 4:472-476.
3. Zhou H., Wu S., Joo J.Y., Zhu S., Han D.W., Lin T., et al. Generation of induced pluripotent stem cells using recombinant proteins. Cell Stem Cell 2009, 4:381-384.
4. Warren L., Manos P.D., Ahfeldt T., Loh Y.H., Li H., Lau F., et al. Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Cell Stem Cell 2010, 7:618-630.
5. Anokye-Danso F., Trivedi C.M., Juhr D., Gupta M., Cui Z., Tian Y., et al. Highly efficient miRNA-mediated reprogramming of mouse and human somatic cells to pluripotency. Cell Stem Cell 2011, 8:376-388.
6. Shi Y., Do J.T., Desponts C., Hahm H.S., Scholer H.R., Ding S. Acombined chemical and genetic approach for the generation of induced pluripotent stem cells. Cell Stem Cell 2008, 2:525-528.
7. Zhu S., Li W., Zhou H., Wei W., Ambasudhan R., Lin T., et al. Reprogramming of human primary somatic cells by OCT4 and chemical compounds. Cell Stem Cell 2010, 7:651-655.
8. Sekiya S., Suzuki A. Direct conversion of mouse fibroblasts to hepatocyte-like cells by defined factors. Nature 2011, 475:390-393.
9. Szabo E., Rampalli S., Risueno R.M., Schnerch A., Mitchell R., Fiebig-Comyn A., et al. Direct conversion of human fibroblasts to multilineage blood progenitors. Nature 2010, 468:521-526.
10. Ieda M., Fu J.D., Delgado-Olguin P., Vedantham V., Hayashi Y., Bruneau B.G., et al. Direct reprogramming of fibroblasts into functional cardiomyocytes by defined factors. Cell 2010, 142:375-386.
11. Liu X., Li F., Stubblefield E.A., Blanchard B., Richards T.L., Larson G.A., et al. Direct reprogramming of human fibroblasts into dopaminergic neuron-like cells. Cell Res 2012, 22:321-332.
12. Pang Z.P., Yang N., Vierbuchen T., Ostermeier A., Fuentes D.R., Yang T.Q., et al. Induction of human neuronal cells by defined transcription factors. Nature 2011, 476:220-223.
13. Caiazzo M., Dell'Anno M.T., Dvoretskova E., Lazarevic D., Taverna S., Leo D., et al. Direct generation of functional dopaminergic neurons from mouse and human fibroblasts. Nature 2011, 476:224-227.
14. Pfisterer U., Kirkeby A., Torper O., Wood J., Nelander J., Dufour A., et al. Direct conversion of human fibroblasts to dopaminergic neurons. Proc Natl Acad Sci U S A 2011, 108:10343-10348.
15. Son E.Y., Ichida J.K., Wainger B.J., Toma J.S., Rafuse V.F., Woolf C.J., et al. Conversion of mouse and human fibroblasts into functional spinal motor neurons. Cell Stem Cell 2011, 9:205-218.
16. Vierbuchen T., Ostermeier A., Pang Z.P., Kokubu Y., Sudhof T.C., Wernig M. Direct conversion of fibroblasts to functional neurons by defined factors. Nature 2010, 463:1035-1041.
17. Kim J., Efe J.A., Zhu S., Talantova M., Yuan X., Wang S., et al. Direct reprogramming of mouse fibroblasts to neural progenitors. Proc Natl Acad Sci U S A 2011, 108:7838-7843.
18. Lujan E., Chanda S., Ahlenius H., Sudhof T.C., Wernig M. Direct conversion of mouse fibroblasts to self-renewing, tripotent neural precursor cells. Proc Natl Acad Sci U S A 2012, 109:2527-2532.
19. Thier M., Worsdorfer P., Lakes Y.B., Gorris R., Herms S., Opitz T., et al. Direct conversion of fibroblasts into stably expandable neural stem cells. Cell Stem Cell 2012, 10:473-479.
20. Han D.W., Tapia N., Hermann A., Hemmer K., Hoing S., Arauzo-Bravo M.J., et al. Direct reprogramming of fibroblasts into neural stem cells by defined factors. Cell Stem Cell 2012, 10:465-472.
21. Sheng C., Zheng Q., Wu J., Xu Z., Wang L., Li W., et al. Direct reprogramming of sertoli cells into multipotent neural stem cells by defined factors. Cell Res 2012, 22:208-218.
22. Ring K.L., Tong L.M., Balestra M.E., Javier R., Andrews-Zwilling Y., Li G., et al. Direct reprogramming of mouse and human fibroblasts into multipotent neural stem cells with a single factor. Cell Stem Cell 2012, 11:100-109.
23. Eshghi S., Schaffer D.V. Engineering microenvironments to control stem cell fate and function. StemBook[Internet]. Cambridge (MA) 2008, Harvard Stem Cell Institure. 10.3824/stembook.1.5.1. S. Bhatia, J. Polak (Eds.).
24. Tavazoie M., Van der Veken L., Silva-Vargas V., Louissaint M., Colonna L., Zaidi B., et al. Aspecialized vascular niche for adult neural stem cells. Cell Stem Cell 2008, 3:279-288.
25. Fuchs E., Tumbar T., Guasch G. Socializing with the neighbors: stem cells and their niche. Cell 2004, 116:769-778.
26. Sarig R., Baruchi Z., Fuchs O., Nudel U., Yaffe D. Regeneration and transdifferentiation potential of muscle-derived stem cells propagated as myospheres. Stem Cells 2006, 24:1769-1778.
27. Li T.S., Cheng K., Lee S.T., Matsushita S., Davis D., Malliaras K., et al. Cardiospheres recapitulate a niche-like microenvironment rich in stemness and cell-matrix interactions, rationalizing their enhanced functional potency for myocardial repair. Stem Cells 2010, 28:2088-2098.
28. Huang G.S., Dai L.G., Yen B.L., Hsu S.H. Spheroid formation of mesenchymal stem cells on chitosan and chitosan-hyaluronan membranes. Biomaterials 2011, 32:6929-6945.
29. Cheng N.C., Wang S., Young T.H. The influence of spheroid formation of human adipose-derived stem cells on chitosan films on stemness and differentiation capabilities. Biomaterials 2012, 33:1748-1758.
30. Su G., Zhao Y., Wei J., Han J., Chen L., Xiao Z., et al. The effect of forced growth of cells into 3D spheres using low attachment surfaces on the acquisition of stemness properties. Biomaterials 2013, 34:3215-3222.
31. Reynolds B.A., Weiss S. Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science 1992, 255:1707-1710.
32. Liu J., Kuznetsova L.A., Edwards G.O., Xu J., Ma M., Purcell W.M., et al. Functional three-dimensional HepG2 aggregate cultures generated from an ultrasound trap: comparison with HepG2 spheroids. JCell Biochem 2007, 102:1180-1189.
33. Gage F.H. Mammalian neural stem cells. Science 2000, 287:1433-1438.
34. Eriksson P.S., Perfilieva E., Bjork-Eriksson T., Alborn A.M., Nordborg C., Peterson D.A., et al. Neurogenesis in the adult human hippocampus. Nat Med 1998, 4:1313-1317.
35. Clarke L., van der Kooy D. Low oxygen enhances primitive and definitive neural stem cell colony formation by inhibiting distinct cell death pathways. Stem Cells 2009, 27:1879-1886.
36. Panchision D.M. The role of oxygen in regulating neural stem cells in development and disease. JCell Physiol 2009, 220:562-568.
37. Keung A.J., Kumar S., Schaffer D.V. Presentation counts: microenvironmental regulation of stem cells by biophysical and material cues. Annu Rev Cell Dev Biol 2010, 26:533-556.
38. Graham V., Khudyakov J., Ellis P., Pevny L. SOX2 functions to maintain neural progenitor identity. Neuron 2003, 39:749-765.
39. Episkopou V. SOX2 functions in adult neural stem cells. Trends Neurosci 2005, 28:219-221.
40. Bylund M., Andersson E., Novitch B.G., Muhr J. Vertebrate neurogenesis is counteracted by Sox1-3 activity. Nat Neurosci 2003, 6:1162-1168.