Neural commitment of human pluripotent stem cells under defined conditions recapitulates neural development and generates patient-specific neural cells

Biotechnology Journal

Volumn 10, Issue 10, 2015, Pages 1578-1588

  Fernandes, Tiago G ^a   Duarte, Sofia T ^b   Ghazvini, Mehrnaz ^c   Gaspar, Cláudia ^b   Santos, Diana C ^a   Porteira, Ana R ^a   Rodrigues, Gonçalo M C ^a   Haupt, Simone ^d   Rombo, Diogo M ^b,e   Armstrong, Judith ^f   Sebastião, Ana M ^b,e   Gribnau, Joost ^c   Garcia Cazorla, Àngels ^f   Brüstle, Oliver ^d   Henrique, Domingos ^b   Cabral, Joaquim M S ^a   Diogo, Maria Margarida ^a  

^a INSTITUTO SUPERIOR TÉCNICO   (Portugal)

^b HOSPITAL DONA ESTEFÂNIA   (Portugal)

^c ERASMUS MEDICAL CENTER   (Netherlands)

^d UNIVERSITY OF BONN   (Germany)

^e INSTITUTO DE MEDICINA MOLECULAR   (Portugal)

^f INSTITUTO DE SALUD CARLOS III   (Spain)

Author keywords

Defined culture conditions; Dual SMAD inhibition; Induced pluripotent stem cells; Neurodevelopment modeling; Rett syndrome

Indexed keywords

CELL CULTURE; CYTOLOGY; NEURONS; STEM CELLS;

CULTURE CONDITIONS; EXPERIMENTAL METHODOLOGY; HUMAN-INDUCED PLURIPOTENT STEM CELLS; INDUCED PLURIPOTENT STEM CELLS; NEURAL DIFFERENTIATIONS; NEURODEVELOPMENT; PLURIPOTENT STEM CELLS; RETT SYNDROME;

CELLS;

INHIBITORY SMAD PROTEIN; KRUPPEL LIKE FACTOR 4; MICROTUBULE ASSOCIATED PROTEIN 2; OCTAMER TRANSCRIPTION FACTOR 4; TRANSCRIPTION FACTOR SOX2; VITRONECTIN; CULTURE MEDIUM; MECP2 PROTEIN, HUMAN; METHYL CPG BINDING PROTEIN 2;

ARTICLE; CELL SPECIFICITY; CONTROLLED STUDY; DOWN REGULATION; ELECTROPHYSIOLOGY; EXTRACELLULAR MATRIX; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; IN VITRO STUDY; NERVE CELL; NERVE CELL CULTURE; NERVE CELL DIFFERENTIATION; NEURAL TUBE; NEUROECTODERM; NEUROEPITHELIUM CELL; ONCOGENE C MYC; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RETT SYNDROME; ROSETTE FORMATION; STEM CELL CULTURE; UPREGULATION; BIOSYNTHESIS; CELL CULTURE TECHNIQUE; CELL DIFFERENTIATION; CELL LINE; CELL PROLIFERATION; CULTURE MEDIUM; CYTOLOGY; EMBRYONIC STEM CELL; GENE EXPRESSION REGULATION; GENETICS; INDUCED PLURIPOTENT STEM CELL; NERVOUS SYSTEM DEVELOPMENT; NEURAL STEM CELL; PATHOLOGY; PROCEDURES;

CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL LINE; CELL PROLIFERATION; CULTURE MEDIA; EMBRYONIC STEM CELLS; GENE EXPRESSION REGULATION, DEVELOPMENTAL; HUMANS; INDUCED PLURIPOTENT STEM CELLS; METHYL-CPG-BINDING PROTEIN 2; NEURAL STEM CELLS; NEUROGENESIS; NEURONS; RETT SYNDROME; SMAD PROTEINS, INHIBITORY;

EID: 84943351938     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201400751     Document Type: Article

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