Active-site engineering of ω-transaminase for production of unnatural amino acids carrying a side chain bulkier than an ethyl substituent

Applied and Environmental Microbiology

Volumn 81, Issue 20, 2015, Pages 6994-7002

  Han, Sang Woo ^a   Park, Eul Soo ^a   Dong, Joo Young ^a   Shin, Jong Shik ^a  

^a Yonsei University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

AMINATION; BINS; MOLECULAR ORIENTATION; SUBSTRATES;

ACTIVE SITE RESIDUES; ALANINE SUBSTITUTION; ALANINE-SCANNING MUTAGENESIS; BINDING ORIENTATION; ENANTIOMERIC EXCESS; OCHROBACTRUM ANTHROPI; OMEGA-TRANSAMINASES; UNNATURAL AMINO ACIDS;

AMINO ACIDS;

AMINO ACID; ENZYME ACTIVITY; MICROBIAL ACTIVITY; MUTATION; ORGANIC COMPOUND;

OCHROBACTRUM ANTHROPI;

AMINO ACID; AMINOTRANSFERASE;

AMINO ACID SUBSTITUTION; BIOSYNTHESIS; ENZYME ACTIVE SITE; ENZYMOLOGY; GENETICS; METABOLISM; MOLECULAR DOCKING; OCHROBACTRUM ANTHROPI; PROTEIN ENGINEERING; SITE DIRECTED MUTAGENESIS;

AMINO ACID SUBSTITUTION; AMINO ACIDS; CATALYTIC DOMAIN; MOLECULAR DOCKING SIMULATION; MUTAGENESIS, SITE-DIRECTED; OCHROBACTRUM ANTHROPI; PROTEIN ENGINEERING; TRANSAMINASES;

EID: 84943279434     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01533-15     Document Type: Article

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