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Volumn 5, Issue 1, 2013, Pages 150-153

Connecting Unexplored Protein Crystal Structures to Enzymatic Function

Author keywords

Amines; Asymmetric synthesis; Enantioselectivity; Enzyme catalysis; Protein structures

Indexed keywords


EID: 84871649774     PISSN: 18673880     EISSN: 18673899     Source Type: Journal    
DOI: 10.1002/cctc.201200544     Document Type: Article
Times cited : (69)

References (36)
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    • 64049109810 scopus 로고    scopus 로고
    • Eds.: S. Lutz, U.T. Bornscheuer), Wiley-VCH, Weinheim
    • Protein Engineering Handbook (Eds.: S. Lutz, U.T. Bornscheuer), Wiley-VCH, Weinheim, 2009;
    • (2009) Protein Engineering Handbook
  • 20
    • 84857650697 scopus 로고    scopus 로고
    • In parallel to the characterization of the four transaminases of this paper, we solved the crystal structure of the amine transaminase of Chromobacterium violaceum; see
    • In parallel to the characterization of the four transaminases of this paper, we solved the crystal structure of the amine transaminase of Chromobacterium violaceum; see M. Svedendahl-Humble, K. E. Cassimjee, M. Hakansson, Y. R. Kimbung, B. Walse, V. Abedi, H. J. Federsel, P. Berglund, D. T. Logan, FEBS J. 2012, 279, 779-792.
    • (2012) FEBS J. , vol.279 , pp. 779-792
    • Svedendahl-Humble, M.1    Cassimjee, K.E.2    Hakansson, M.3    Kimbung, Y.R.4    Walse, B.5    Abedi, V.6    Federsel, H.J.7    Berglund, P.8    Logan, D.T.9
  • 31
    • 84871652078 scopus 로고    scopus 로고
    • Our attempts to characterize the substrate scope and enantioselectivity towards different chiral amines failed, as this Pseudomonas putida ω-amino acid transaminase could not be expressed in E. coli in a soluble form (a synthetic, codon-optimized gene was used and various conditions, e.g, temperature, chaperones, were investigated).
    • Our attempts to characterize the substrate scope and enantioselectivity towards different chiral amines failed, as this Pseudomonas putida ω-amino acid transaminase could not be expressed in E. coli in a soluble form (a synthetic, codon-optimized gene was used and various conditions, e.g, temperature, chaperones, were investigated).
  • 36
    • 84871656501 scopus 로고    scopus 로고
    • F. Steffen-Munsberg, C. Vickers, A. Thontowi, S. Schätzle, T. Meinhardt, M. Svedendahl Humble, H. Land, P. Berglund, U.T. Bornscheuer, M. Höhne, ChemCatchem 2012, DOI:
    • F. Steffen-Munsberg, C. Vickers, A. Thontowi, S. Schätzle, T. Meinhardt, M. Svedendahl Humble, H. Land, P. Berglund, U.T. Bornscheuer, M. Höhne, ChemCatchem 2012, DOI:


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.