CenC, a multidomain thermostable GH9 processive endoglucanase from Clostridium thermocellum: cloning, characterization and saccharification studies

World Journal of Microbiology and Biotechnology

Volumn 31, Issue 11, 2015, Pages 1699-1710

  Haq, Ikram ul ^a   Akram, Fatima ^a   Khan, Mahmood Ali ^a   Hussain, Zahid ^a   Nawaz, Ali ^a   Iqbal, Kaleem ^a   Shah, Ali Javed ^a  

^a GC UNIVERSITY   (Pakistan)

Author keywords

Clostridium thermocellum; Module protein; Processive endoglucanase; Saccharification; Thermostable

Indexed keywords

BIODEGRADATION; BIOMASS; CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; CLONING; CLOSTRIDIUM; COST EFFECTIVENESS; ENZYMES; ESCHERICHIA COLI; ION CHROMATOGRAPHY; ION EXCHANGE; PURIFICATION; SACCHARIFICATION; SUBSTRATES;

BIODEGRADATION PROCESS; CARBOXY-METHYL CELLULOSE; CATALYTIC EFFICIENCIES; CLOSTRIDIUM THERMOCELLUM; ENDOGLUCANASES; ION EXCHANGE CHROMATOGRAPHY; THERMOSTABLE; WHATMAN FILTER PAPERS;

ENZYME ACTIVITY;

BACTERIAL PROTEIN; CELLULASE; RECOMBINANT PROTEIN;

CHEMISTRY; CLOSTRIDIUM THERMOCELLUM; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; METABOLISM; MOLECULAR CLONING; PROCEDURES; TEMPERATURE;

BACTERIAL PROTEINS; CELLULASE; CLONING, MOLECULAR; CLOSTRIDIUM THERMOCELLUM; ENZYME STABILITY; ESCHERICHIA COLI; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 84942372086     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-015-1920-4     Document Type: Article

References (43)

1. Bajaj BK, Pangotra H, Wani AM, Sharma P, Sharma A (2009) Partial purification and characterization of a highly thermostable and pH stable endoglucanase from a newly isolated Bacillus strain M-9. Indian J Chem Technol 16:382–387
2. Bradford MM (1976) A dye binding assay for protein. Anal Biochem 72:248–254
3. Downing M, Eaton LM, Graham RL, Langholtz MH, Perlack RD, Turhollow AF, Stokes B, Brandt CC (2011) US billion-ton update: the biomass supply for a bioenergy and bioproducts industry. Oak Ridge National Laboratory, Oak Ridge
4. El-Naghy MA, El-Katatny MS, Attia AA (1991) Degradation of cellulosic materials by Sporotrichum thermophile culture filtrate for sugar production. Int Biodeterioration 27(1):75–79
5. Gaudin C, Belaich A, Champ S, Belaich JP (2000) CelE, a multidomain cellulase from Clostridium cellulolyticum: a Key Enzyme in the Cellulosome? J Bacteriol 182(7):1910–1915
6. Gilad R, Rabinovich L, Yaron S, Edward A, Lamed R, Gilbert HJ, Shoham Y (2003) CelI, a noncellulosomal family 9 enzyme from Clostridium thermocellum, is a processive endoglucanase that degrades crystalline cellulose. J Bacteriol 185(2):391–398
7. Iqbal NMH, Ahmed I, Zia AM, Irfan M (2011) Purification and characterization of the kinetic parameters of cellulase produced from wheat straw by Trichoderma viride under SSF and its detergent compatibility. Adv Biosci Biotechnol 2:149–156
8. Ja’afaru MI, Fagade OE (2007) Cellulase production and enzymatic hydrolysis of some selected local lignocellulosic substrates by a strain of Aspergillus niger. Res J Bio Sci 2(1):13–16
9. Kataeva I, Li X-L, Chen H, Ljungdahl LG (1999) Cloning and sequence analysis of a new cellulase gene encoding CelK, a major cellulosome component of Clostridium thermocellum: evidence for gene duplication and recombination. J Bacteriol 181:5288–5295
10. Kataeva IA, Uversky VN, Brewer JM, Schubot F, Rose JP, Wang BC, Ljungdahl LG (2004) Interactions between immunoglobulin-like and catalytic modules in Clostridium thermocellum cellulosomal cellobiohydrolase CbhA. Protein Eng 17(11):759–769
11. Khademi S, Guarino LA, Watanabe H, Tokuda G, Meyer EF (2002) Structure of an endoglucanase from termite, Nasutitermes takasagoensis. Acta Crystallogr D Biol Crystallogr 58:653–659
12. Kibbe WA (2007) OligoCalc: an online oligonucleotide properties calculator. Nucleic Acids Res 35(Suppl 2):W43–W46. doi:10.1093/nar/gkm234
13. Kocher GS, Kalra KL (2013) Optimization of pretreatment, enzymatic saccharification and fermentation conditions for bioethanol production from rice straw. Ind J Appl Res 3(5):62–64
14. Koppram R, Pejó ET, Xiros C, Olsson L (2014) Lignocellulosic ethanol production at high-gravity: challenges and perspectives. Trends Biotechnol 32(1):46–53
15. Liang C, Fioronic M, Rodriguez-Roperoc F, Xuea Y, Schwanebergc U, Maa Y (2011) Directed evolution of a thermophilic endoglucanase (Cel5A) into highly active Cel5A variants with an expanded temperature profile. J Biotechnol 154:46–53
16. Liu J, Liu W, Zhao X (2011) Cloning and functional characterization of a novel endo-β-1,4-glucanase gene from a soil-derived metagenomic library. App Microbiol Biotechnol 89:1083–1092
17. Liu D, Zhang R, Yang X, Zhang Z, Song S, Miao Y, Shen Q (2012) Characterization of a thermostable β-glucosidase from Aspergillus fumigatus Z5, and its functional expression in Pichia pastoris X33. Microb Cell Fact 11:25
18. Maki M, Leung KT, Qin W (2009) The prospects of cellulase-producing bacteria for the bioconversion of lignocellulosic biomass. Int J Biol Sci 5(5):500–516
19. Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428
20. Morana A, Esposito A, Maurelli L, Ruggiero G, Ionata E, Rossi M, La Cara L (2008) A novel thermoacidophilic cellulase from Alicyclobacillus acidocaldarius. Protein Pept Lett 15:1017–1021
21. Narraa M, Dixit G, Divecha J, Kumar K, Madamwar D, Shah AR (2014) Production, purification and characterization of a novel GH 12 family endoglucanase from Aspergillus terreus and its application in enzymatic degradation of delignified rice straw. Int Biodeterior Biodegrad 88:150–161
22. Parsiegla G, Juy M, Reverbel-Leroy C, Tardif C, Belaich JP, Driguez H, Haser R (1998) The crystal structure of the processive Endocellulase, CelF, of Clostridium cellulolyticum in complex with a thio-oligosaccharide inhibitor at 2.0 A° resolution. EMBO J 17:5551–5562
23. Pham TH, Quyen DT, Nghiem NM (2012) Purification and properties of an endoglucanase from Aspergillus niger VTCC-F021. Turk J Biol 36:694–701
24. Reverbel-Leroy C, Pages S, Belaich A, Belaich JP, Tardif C (1997) The processive endocellulase CelF, a major component of the Clostridium cellulolyticum cellulosome, purification and characterization of the recombinant form. J Bacteriol 179:46–52
25. Riederer A, Takasuka TE, Makino S, Stevenson DM, Bukhman YV, Elsen NL, Fox BG (2011) Global gene expression patterns in Clostridium thermocellum as determined by microarray analysis of chemostat cultures on cellulose or cellobiose. Appl Environ Microbiol 77(4):1243–1253
26. Sadhu S, Saha P, Sen SK, Mayilraj S, Maiti TK (2013) Production, purification and characterization of a novel thermotolerant endoglucanase (CMCase) from Bacillus strain isolated from cow dung. Springer Plus 2(1):1–10
27. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor, New York
28. Selig M, Weiss N, Ji Y (2008) Enzymatic saccharification of lignocellulosic biomass. NREL Ethanol Project CAT Task Laboratory Analytical Procedure #009
29. Shi R, Li Z, Ye Q, Xu J, Liu Y (2013) Heterologous expression and characterization of a novel thermo-halotolerant endoglucanase Cel5H from Dictyoglomus thermophilum. Bioresour Technol 142:338–344
30. Shi H, Zhang Y, Wang L, Li X, Li W, Wang F, Li X (2014) Molecular analysis of hyperthermophilic endoglucanase Cel12B from Thermotoga maritima and the properties of its functional residues. BMC Struct Biol 14:8
31. Silva VF, Arruda PV, Felipe MG, Gonçalves AR, Rocha GJ (2011) Fermentation of cellulosic hydrolysates obtained by enzymatic saccharification of sugarcane bagasse pretreated by hydrothermal processing. J Ind Microbiol Biotechnol 38(7):809–817
32. Te’o VSJ, Saul DJ, Bergquist PL (1995) CelA, another gene coding for a multidomain cellulase from the extreme thermophile Caldocellum saccharolyticum. Appl Microbiol Biotechnol 43:291–296
33. Vallander L, Eriksson KE (1985) Enzymic saccharification of pretreated wheat straw. Biotechnol Bioeng 27(5):650–659
34. Várnai A, Tang C, Bengtsson O, Atterton A, Mathiesen G, Vincent GH (2014) Expression of endoglucanases in Pichia pastoris under control of the GAP promoter. Microb Cell Fact 13:57
35. Watson BJ, Haitao Z, Atkinson GL, Young HM, Steven WH (2009) Processive Endoglucanases Mediate Degradation of Cellulose by Saccharophagus degradans. J Bacteriol 191(18):5697–5705
36. Wei KSC, Teoh TC, Koshy P, Salmah I, Zainudin A (2015) Cloning, expression and characterization of the endoglucanase gene from Bacillus subtilis UMC7 isolated from the gut of the indigenous termite Macrotermes malaccensis in Escherichia coli. Electro J Biotechnol 18:103–109
37. Wolfgang DE, Wilson DB (1999) Mechanistic studies of active site mutants of Thermomonospora fusca endocellulase E2. Biochemistry 38:9746–9751
38. Wonganu B, Pootanakit K, Booyapakron K, Champreda V, Tanaponqpipat S, Eurwilaichitr L (2008) Cloning, expression and characterization of a thermotolerant endoglucanase from Syncephalastrum racemosum (BCC18080) in Pichia pastoris. Protein Expr Purif 58:78–86
39. Yennamalli RM, Rader AJ, Kenny AJ, Wolt JD, Sen TZ (2013) Endoglucanases: insights into thermostability for biofuel applications. Biotechnol Biofuels 6:136
40. Zafar M, Ahmed S, Khan MI, Jamil A (2014) Recombinant expression and characterization of a novel endoglucanase from Bacillus subtilis in Escherichia coli. Mol Biol Rep 41:3295–3302
41. Zhang X-Z, Sathitsuksanoh N, Zhang Y-HP (2010) Glycoside hydrolase family 9 processive endoglucanase from Clostridium phytofermentans: Heterologous expression, characterization, and synergy with family 48 cellobiohydrolase. Bioresour Technol 101:5534–5538
42. Zheng B, Yang W, Wang Y, Feng Y, Lou Z (2009) Crystallization and preliminary crystallographic analysis of thermophilic cellulase from Fervidobacterium nodosum Rt17-B1. Acta Crystallogr Sect F Struct Biol Cryst Commun 65:219–222
43. Zhou WL, Irwin DC, Escovar-Kousen J, Wilson DB (2004) Kinetic studies of Thermobifida fusca Cel9A active site mutant enzymes. Biochem 43(30):9655–9663