Molecular cloning, heterologous expression, and functional characterization of an NADPH-cytochrome P450 reductase gene from Camptotheca acuminata, a camptothecin-producing plant

PLoS ONE

Volumn 10, Issue 8, 2015, Pages

  Qu, Xixing ^a,c   Pu, Xiang ^a,c   Chen, Fei ^a,c   Yang, Yun ^a,c   Yang, Lixia ^a,c   Zhang, Guolin ^a   Luo, Yinggang ^a,b  

^a CHENGDU INSTITUTE OF BIOLOGY   (China)

^b SHANGHAI INSTITUTE OF ORGANIC CHEMISTRY   (China)

^c INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; CYP 73A25; CYTOCHROME; CYTOCHROME C; FERRICYANIDE; RECOMBINANT ENZYME; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE FERRIHEMOPROTEIN REDUCTASE; UNCLASSIFIED DRUG; ANTINEOPLASTIC AGENT; CAMPTOTHECIN; RECOMBINANT PROTEIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE FERRIHEMOPROTEIN REDUCTASE; TRYPTOPHAN;

AMINO TERMINAL SEQUENCE; ARTICLE; BIOSYNTHESIS; CAMPTOTHECA; CAMPTOTHECA ACUMINATA; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE EXPRESSION; GENE FUNCTION; GENETIC ANALYSIS; HYDROPHOBICITY; MOLECULAR CLONING; NADPH CYTOCHROME P450 REDUCTASE GENE; NONHUMAN; OPEN READING FRAME; OXIDATION KINETICS; PLANT GENE; PLANT LEAF; PLANT ROOT; PLANT STEM; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; UPREGULATION; AMINO ACID SEQUENCE; CATALYSIS; CHEMISTRY; CONFORMATION; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; MOLECULAR GENETICS; MOLECULAR MODEL; PHYLOGENY; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; ANTINEOPLASTIC AGENTS; CAMPTOTHECA; CAMPTOTHECIN; CATALYSIS; CLONING, MOLECULAR; CYTOCHROMES C; DNA, COMPLEMENTARY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, PLANT; MODELS, MOLECULAR; MOLECULAR CONFORMATION; MOLECULAR SEQUENCE DATA; NADPH-FERRIHEMOPROTEIN REDUCTASE; OPEN READING FRAMES; PHYLOGENY; PLANT LEAVES; PLANT ROOTS; PLANT STEMS; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; TRYPTOPHAN;

EID: 84941963973     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0135397     Document Type: Article

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