Isolation of murine peritoneal macrophages to carry out gene expression analysis upon toll-like receptors stimulation

Journal of Visualized Experiments

Volumn 2015, Issue 98, 2015, Pages

  Layoun, Antonio ^a   Samba, Macha ^a   Santos, Manuela M ^a,b  

^a CENTRE HOSPITALIER DE L UNIVERSITÉ DE MONTRÉAL   (Canada)

^b UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

Immunology; Infection; Inflammation; Issue 98; Macrophages; Peritoneal cavity; RNA extraction; Thioglycollate; TLRs

Indexed keywords

LIGAND; MESSENGER RNA; TOLL LIKE RECEPTOR;

ANIMAL; BIOSYNTHESIS; CELL CULTURE; CYTOLOGY; DRUG EFFECTS; GENE EXPRESSION; GENETICS; IMMUNOLOGY; ISOLATION AND PURIFICATION; MACROPHAGE ACTIVATION; METABOLISM; MONOCYTE; MOUSE; PERITONEUM MACROPHAGE; PHYSIOLOGY; PROCEDURES; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SPLEEN;

ANIMALS; CELLS, CULTURED; CYTOLOGICAL TECHNIQUES; GENE EXPRESSION; LIGANDS; MACROPHAGE ACTIVATION; MACROPHAGES, PERITONEAL; MICE; MONOCYTES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SPLEEN; TOLL-LIKE RECEPTORS;

EID: 84941255029     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/52749     Document Type: Article

References (29)

1. Pollard, J. W. Trophic macrophages in development and disease. Nat Rev Immunol. 9, (4), 259-270 (2009).
2. Gordon, S. Alternative activation of macrophages. Nat Rev Immunol. 3, (1), 23-35 (2003).
3. Koury, M. J., Ponka, P. New insights into erythropoiesis: the roles of folate, vitamin B12, and iron. Annu Rev Nutr. 24, 105-131 (2004).
4. Sheftel, A. D., Kim, S. F., Ponka, P. Non-heme induction of heme oxygenase-1 does not alter cellular iron metabolism. J Biol Chem. 282, (14), 10480-10486 (2007).
5. Hershko, C. Storage iron regulation. Prog Hematol. 10, 105-148 (1977).
6. Collins, H. L. Withholding iron as a cellular defence mechanism--friend or foe. Eur J Immunol. 38, (7), 1803-1806 (2008).
7. Noyes, W. D., Bothwell, T. H., Finch, C. A. The role of the reticulo-endothelial cell in iron metabolism. Br J Haematol. 6, 43-55 (1960).
8. Layoun, A., Huang, H., Calve, A., Santos, M. M. Toll-like receptor signal adaptor protein MyD88 is required for sustained endotoxin-induced acute hypoferremic response in mice. Am J Pathol. 180, (6), 2340-2350 (2012).
9. Zumerle, S., et al. Targeted disruption of hepcidin in the liver recapitulates the hemochromatotic phenotype. Blood. 123, (23), 3646-3650 (2014).
10. Nguyen, N. B., Callaghan, K. D., Ghio, A. J., Haile, D. J., Yang, F. Hepcidin expression and iron transport in alveolar macrophages. Am J Physiol Lung Cell Mol Physiol. 291, (3), L417-L425 (2006).
11. Nemeth, E., et al. Hepcidin, a putative mediator of anemia of inflammation, is a type II acute-phase protein. Blood. 101, (7), 2461-2463 (2003).
12. Nemeth, E., et al. Hepcidin regulates cellular iron efflux by binding to ferroportin and inducing its internalization. Science. 306, (5704), 2090-2093 (2004).
13. Constante, M., et al. Distinct requirements for Hfe in basal and induced hepcidin levels in iron overload and inflammation. Am J Physiol Gastrointest Liver Physiol. 291, (2), G229-G237 (2006).
14. Zhang, X., Goncalves, R., Mosser, D. M. The isolation and characterization of murine macrophages. Curr Protoc Immunol. Chapter 14, (Unit 14 11), (2008).
15. Ray, A., Dittel, B. N. Isolation of mouse peritoneal cavity cells. J Vis Exp. (35), (2010).
16. Leijh, P. C., van Zwet, T. L., ter Kuile, M. N., van Furth, R. Effect of thioglycolate on phagocytic and microbicidal activities of peritoneal macrophages. Infect Immun. 46, (2), 448-452 (1984).
17. Dy, M., Debray-Sachs, M., Kamoun, P., Hamburger, J. Effect of thioglycollate on macrophage lysosomal enzymes. Biomedicine. 29, (5), 167-170 (1978).
18. Li, Y. M., Baviello, G., Vlassara, H., Mitsuhashi, T. Glycation products in aged thioglycollate medium enhance the elicitation of peritoneal macrophages. J Immunol Methods. 201, (2), 183-188 (1997).
19. Layoun, A., Santos, M. M. Bacterial cell wall constituents induce hepcidin expression in macrophages through MyD88 signaling. Inflammation. 35, (4), 1500-1506 (2012).
20. Oppenheim, J. J., Yang, D. Alarmins: chemotactic activators of immune responses. Curr Opin Immunol. 17, (4), 359-365 (2005).
21. Akira, S., Uematsu, S., Takeuchi, O. Pathogen recognition and innate immunity. Cell. 124, (4), 783-801 (2006).
22. Lang, R., Patel, D., Morris, J. J., Rutschman, R. L., Murray, P. J. Shaping gene expression in activated and resting primary macrophages by IL-10. J Immunol. 169, (5), 2253-2263 (2002).
23. Wang, C., et al. Characterization of murine macrophages from bone marrow, spleen and peritoneum. BMC Immunol. 14, (6), (2013).
24. Austin, P. E., McCulloch, E. A., Till, J. E. Characterization of the factor in L-cell conditioned medium capable of stimulating colony formation by mouse marrow cells in culture. J Cell Physiol. 77, (2), 121-134 (1971).
25. Cannon, G. J., Swanson, J. A. The macrophage capacity for phagocytosis. J Cell Sci. 101, (Pt 4), 907-913 (1992).
26. Wang, Y., Harris, D. C. Macrophages in renal disease. J Am Soc Nephrol. 22, (1), 21-27 (2011).
27. Edwards, J. P., Zhang, X., Frauwirth, K. A., Mosser, D. M. Biochemical and functional characterization of three activated macrophage populations. J Leukoc Biol. 80, (6), 1298-1307 (2006).
28. Hoover, D. L., Nacy, C. A. Macrophage activation to kill Leishmania tropica: defective intracellular killing of amastigotes by macrophages elicited with sterile inflammatory agents. J Immunol. 132, (3), 1487-1493 (1984).
29. Schleicher, U., Bogdan, C. Generation culture and flow-cytometric characterization of primary mouse macrophages. Methods Mol Biol. 531, 203-224 (2009).