Methods for culturing human femur tissue explants to study breast cancer cell colonization of the metastatic niche

Journal of Visualized Experiments

Volumn 2015, Issue 97, 2015, Pages

  Templeton, Zachary S ^a   Bachmann, Michael H ^a   Alluri, Rajiv V ^a   Maloney, William J ^a   Contag, Christopher H ^a   King, Bonnie L ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Bioluminescence imaging; Bone microenvironment; Breast cancer metastasis; Ex vivo model; Explant culture system; Human bone; Issue 97; Medicine; Metastatic niche; Osteotropism

Indexed keywords

ADULT; AGED; BONE TUMOR; BREAST TUMOR; CANCER STEM CELL; CELL MOTION; CYTOLOGY; FEMALE; FEMUR; FLOW CYTOMETRY; HUMAN; MALE; METASTASIS; MIDDLE AGED; PATHOLOGY; PHYSIOLOGY; PROCEDURES; SECONDARY; STEM CELL NICHE; TISSUE CULTURE TECHNIQUE; TUMOR CELL LINE; TUMOR MICROENVIRONMENT; VERY ELDERLY;

ADULT; AGED; AGED, 80 AND OVER; BONE NEOPLASMS; BREAST NEOPLASMS; CELL LINE, TUMOR; CELL MOVEMENT; FEMALE; FEMUR; FLOW CYTOMETRY; HUMANS; MALE; MIDDLE AGED; NEOPLASM METASTASIS; NEOPLASTIC STEM CELLS; STEM CELL NICHE; TISSUE CULTURE TECHNIQUES; TUMOR MICROENVIRONMENT;

EID: 84941242477     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/52656     Document Type: Article

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