Recommended mass spectrometry-based strategies to identify botulinum neurotoxin-containing samples

Toxins

Volumn 7, Issue 5, 2015, Pages 1765-1778

  Kalb, Suzanne R ^a   Baudys, Jakub ^a   Wang, Dongxia ^a   Barr, John R ^a  

^a CENTERS FOR DISEASE CONTROL AND PREVENTION   (United States)

Author keywords

Botulinum neurotoxin; Botulism; Mass spectrometry

Indexed keywords

BOTULINUM TOXIN; CHYMOTRYPSIN; SYNTAXIN; TRYPSIN; MONOCLONAL ANTIBODY;

ANTIBODY AFFINITY; ARTICLE; BOTULISM; CONTROLLED STUDY; ENZYME ACTIVITY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; NERVE CONDUCTION; NONHUMAN; PROTEIN ANALYSIS; QUALITATIVE ANALYSIS; SEQUENCE ANALYSIS; AMINO ACID SEQUENCE; ANALYSIS; ANIMAL; BLOOD; CHEMISTRY; FOOD CONTAMINATION; IMMUNOLOGY; MEAT; MILK; PROCEDURES;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; BOTULINUM TOXINS; FOOD CONTAMINATION; MASS SPECTROMETRY; MEAT; MILK;

EID: 84941200363     PISSN: None     EISSN: 20726651     Source Type: Journal    
DOI: 10.3390/toxins7051765     Document Type: Article

References (34)

1. Binz, T.; Blasi, J.; Yamasaki, S.; Baumeister, A.; Link, E.; Sudhof, T.C.; Jahn, R.; Niemann, H. Proteolysis of SNAP-25 by types E and A botulinal neurotoxins. J. Biol. Chem. 1994, 269, 1617-1620.
2. Blasi, J.; Chapman, E.R.; Link, E.; Binz, T.; Yamasaki, S.; De Camilli, P.; Sudhof, T.C.; Niemann, H.; Jahn, R. Botulinum neurotoxin A selectively cleaves the synaptic protein SNAP-25. Nature 1993, 365, 160-163.
3. Foran, P.; Lawrence, G.W.; Shone, C.C.; Foster, K.A.; Dolly, J.O. Botulinum neurotoxin C1 cleaves both syntaxin and SNAP-25 in intact and permeabilized chromaffin cells: Correlation with its blockade of catecholamine release. Biochemistry 1996, 35, 2630-2636.
4. Schiavo, G.; Rossetto, O.; Catsicas, S.; Polverino de Laureto, P.; DasGupta, B.R.; Benfenati, F.; Montecucco, C. Identification of the nerve terminal targets of botulinum neurotoxin serotypes A, D, and E. J. Biol. Chem. 1993, 268, 23784-23787.
5. Schiavo, G.; Santucci, A.; Dasgupta, B.R.; Mehta, P.P.; Jontes, J.; Benfenati, F.; Wilson, M.C.; Montecucco, C. Botulinum neurotoxins serotypes A and E cleave SNAP-25 at distinct COOH-terminal peptide bonds. FEBS Lett. 1993, 335, 99-103.
6. Williamson, L.C.; Halpern, J.L.; Montecucco, C.; Brown, J.E.; Neale, E.A. Clostridial neurotoxins and substrate proteolysis in intact neurons: Botulinum neurotoxin C acts on synaptosomal-associated protein of 25 kDa. J. Biol. Chem. 1996, 271, 7694-7699.
7. Kalb, S.R.; Baudys, J.; Webb, R.P.; Wright, P.; Smith, T.J.; Smith, L.A.; Fernandez, R.; Raphael, B.H.; Maslanka, S.E.; Pirkle, J.L.; et al. Discovery of a novel enzymatic cleavage site for botulinum neurotoxin F5. FEBS Lett. 2012, 586, 109-115.
8. Schiavo, G.; Benfenati, F.; Poulain, B.; Rossetto, O.; Polverino de Laureto, P.; DasGupta, B.R.; Montecucco, C. Tetanus and botulinum-B neurotoxins block neurotransmitter release by proteolytic cleavage of synaptobrevin. Nature 1992, 359, 832-835.
9. Schiavo, G.; Malizio, C.; Trimble, W.S.; Polverino de Laureto, P.; Milan, G.; Sugiyama, H.; Johnson, E.A.; Montecucco, C. Botulinum G neurotoxin cleaves VAMP/synaptobrevin at a single Ala-Ala peptide bond. J. Biol. Chem. 1994, 269, 20213--20216.
10. Schiavo, G.; Shone, C.C.; Rossetto, O.; Alexander, F.C.; Montecucco, C. Botulinum neurotoxin serotype F is a zinc endopeptidase specific for VAMP/synaptobrevin. J. Biol. Chem. 1993, 268, 11516-11519.
11. Yamasaki, S.; Baumeister, A.; Binz, T.; Blasi, J.; Link, E.; Cornille, F.; Roques, B.; Fykse, E.M.; Sudhof, T.C.; Jahn, R.; et al. Cleavage of members of the synaptobrevin/VAMP family by types D and F botulinal neurotoxins and tetanus toxin. J. Biol. Chem. 1994, 269, 12764-12772.
12. Yamasaki, S.; Binz, T.; Hayashi, T.; Szabo, E.; Yamasaki, N.; Eklund, M.; Jahn, R.; Niemann, H. Botulinum neurotoxin type G proteolyses the Ala81-Ala82 bond of rat synaptobrevin 2. Biochem. Biophys. Res. Commun. 1994, 200, 829-835.
13. Blasi, J.; Chapman, E.R.; Yamasaki, S.; Binz, T.; Niemann, H.; Jahn, R. Botulinum neurotoxin C1 blocks neurotransmitter release by means of cleaving hpc-1/syntaxin. EMBO J. 1993, 12, 4821-4828.
14. Schiavo, G.; Shone, C.C.; Bennett, M.K.; Scheller, R.H.; Montecucco, C. Botulinum neurotoxin type C cleaves a single Lys-Ala bond within the carboxyl-terminal region of syntaxins. J. Biol. Chem. 1995, 270, 10566-10570.
15. Barr, J.R.; Moura, H.; Boyer, A.E.; Woolfitt, A.R.; Kalb, S.R.; Pavlopoulos, A.; McWilliams, L.G.; Schmidt, J.G.; Martinez, R.A.; Ashley, D.L. Botulinum neurotoxin detection and differentiation by mass spectrometry. Emerg. Infect. Dis. 2005, 11, 1578-1583.
16. Kalb, S.R.; Moura, H.; Boyer, A.E.; McWilliams, L.G.; Pirkle, J.L.; Barr, J.R. The use of endopep-ms for the detection of botulinum toxins A, B, E, and F in serum and stool samples. Anal. Biochem. 2006, 351, 84-92.
17. Kalb, S.R.; Smith, T.J.; Moura, H.; Hill, K.; Lou, J.; Geren, I.N.; Garcia-Rodriguez, C.; Marks, J.D.; Smith, L.A.; Pirkle, J.L.; et al. The use of endopep-ms to detect multiple subtypes of botulinum neurotoxins A, B, E, and F. Int. J. Mass Spectrom. 2008, 278, 101-108.
18. Kalb, S.R.; Krilich, J.C.; Dykes, J.K.; Luquez, C.; Maslanka, S.E.; Barr, J.R. Detection of botulinum toxins A, B, E, and F in foods by Endopep-ms. J. Agric. Food Chem. 2015, [Epub ahead of print].
19. Kalb, S.R.; Santana, W.I.; Pirkle, J.L.; Barr, J.R. Detection, differentiation, and subtyping of botulinum toxins A, B, E, and F by mass spectrometry. Botulism J. 2012, 2, 119-134.
20. Kalb, S.R.; Garcia-Rodriguez, C.; Lou, J.; Baudys, J.; Smith, T.J.; Marks, J.D.; Smith, L.A.; Pirkle, J.L.; Barr, J.R. Extraction of Bont/A, /B, /E, and /F with a single, high affinity monoclonal antibody for detection of botulinum neurotoxin by Endopep-ms. PLoS One 2010, 5, e12237.
21. Kalb, S.R.; Lou, J.; Garcia-Rodriguez, C.; Geren, I.N.; Smith, T.J.; Moura, H.; Marks, J.D.; Smith, L.A.; Pirkle, J.L.; Barr, J.R. Extraction and inhibition of enzymatic activity of botulinum neurotoxins/A1, /A2, and /A3 by a panel of monoclonal anti-Bont/A antibodies. PLoS One 2009, 4, e5355.
22. Kalb, S.R.; Santana, W.I.; Geren, I.N.; Garcia-Rodriguez, C.; Lou, J.; Smith, T.J.; Marks, J.D.; Smith, L.A.; Pirkle, J.L.; Barr, J.R. Extraction and inhibition of enzymatic activity of botulinum neurotoxins /B1, /B2, /B3, /B4, and /B5 by a panel of monoclonal anti-Bont/B antibodies. BMC Biochem. 2011, 12, 58.
23. Parks, B.A.; Shearer, J.D.; Baudys, J.; Kalb, S.R.; Sanford, D.C.; Pirkle, J.L.; Barr, J.R. Quantification of botulinum neurotoxin serotypes A and B from serum using mass spectrometry. Anal. Chem. 2011, 83, 9047-9053.
24. Wang, D.; Baudys, J.; Krilich, J.; Smith, T.J.; Barr, J.R.; Kalb, S.R. A two-stage multiplex method for quantitative analysis of botulinum neurotoxins type A, B, E, and F by MALDI-TOF mass spectrometry. Anal. Chem. 2014, 86, 10847-10854.
25. Boyer, A.E.; Moura, H.; Woolfitt, A.R.; Kalb, S.R.; McWilliams, L.G.; Pavlopoulos, A.; Schmidt, J.G.; Ashley, D.L.; Barr, J.R. From the mouse to the mass spectrometer: Detection and differentiation of the endoproteinase activities of botulinum neurotoxins A-G by mass spectrometry. Anal. Chem. 2005, 77, 3916-3924.
26. Kalb, S.R.; Baudys, J.; Raphael, B.H.; Dykes, J.K.; Luquez, C.; Maslanka, S.E.; Barr, J.R. Functional characterization of botulinum neurotoxin serotype H as a hybrid of known serotypes F and A (Bont F/A). Anal. Chem. 2015, 87, 3911-3917.
27. Maslanka, S.E.; Luquez, C.; Dykes, J.K.; Tepp, W.H.; Pier, C.L.; Pellett, S.; Raphael, B.H.; Kalb, S.R.; Barr, J.R.; Rao, A.; et al. A novel botulinum toxin, previously reported as serotype H, has a hybrid structure of known serotypes A and F that is neutralized with serotype A antitoxin. J. Infectious Dis. 2015, in press.
28. Kalb, S.R.; Baudys, J.; Smith, T.J.; Smith, L.A.; Barr, J.R. Three enzymatically active neurotoxins of clostridium botulinum strain Af84: Bont/A2, /F4, and /F5. Anal. Chem. 2014, 86, 3254-3262.
29. Wang, D.; Baudys, J.; Kalb, S.R.; Barr, J.R. Improved detection of botulinum neurotoxin type A in stool by mass spectrometry. Anal. Biochem. 2011, 412, 67-73.
30. Maslanka, S.E.; Luquez, C.; Raphael, B.H.; Dykes, J.K.; Joseph, L.A., Utility of botulinum toxin ELISA A, B, E, F kits for clinical laboratory investigations of human botulism. Botulism J. 2011, 2, 72-92.
31. Weisemann, J.; Krez, N.; Fiebig, U.; Worbs, S.; Skiba, M.; Dorner, M.B.; Bergstrom, T.; Munoz, A.; Zegers, I.; Fikri, Y.; et al. Generation and characterisation of six recombinant botulinum neurotoxins as reference material for an international proficiency test organized by the EQuATox consortium. 2015, manuscript in preparation.
32. Whitemarsh, R.C.; Tepp, W.H.; Bradshaw, M.; Lin, G.; Pier, C.L.; Scherf, J.M.; Johnson, E.A.; Pellett, S. Characterization of botulinum neurotoxin A subtypes 1 through 5 by investigation of activities in mice, in neuronal cell cultures, and in vitro. Infect. Immun. 2013, 81, 3894-3902.
33. Kull, S.; Schulz, K.M.; Strotmeier, J.W.; Kirchner, S.; Schreiber, T.; Bollenbach, A.; Dabrowski, P.W.; Nitsche, A.; Kalb, S.R.; Dorner, M.B.; et al. Isolation and functional characterization of the novel clostridium botulinum neurotoxin a8 subtype. PLoS One 2015, 10, e0116381.
34. Hunt, D.F.; Yates, J.R., 3rd; Shabanowitz, J.; Winston, S.; Hauer, C.R. Protein sequencing by tandem mass spectrometry. Proc. Natl. Acad. Sci. USA 1986, 83, 6233-6237.