Ca2+-independent sortase-A exhibits high selective protein ligation activity in the cytoplasm of Escherichia coli

Biotechnology Journal

Volumn 10, Issue 9, 2015, Pages 1487-1492

  Hirakawa, Hidehiko ^a   Ishikawa, Suguru ^a   Nagamune, Teruyuki ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

Calcium ion; Mutant; Protein ligation; Sortase A; Staphylococcus aureus

Indexed keywords

CATALYSIS; CELLS; CYTOLOGY; ESCHERICHIA COLI; PEPTIDES;

CALCIUM IONS; MUTANT; PROTEIN LIGATION; SORTASE A; STAPHYLOCOCCUS AUREUS;

CATALYST ACTIVITY;

ACYLTRANSFERASE; BACTERIAL ENZYME; CALCIUM ION; MUTANT PROTEIN; SORTASE A; UNCLASSIFIED DRUG; AMINOACYLTRANSFERASE; BACTERIAL PROTEIN; CALCIUM; CYSTEINE PROTEINASE; PROTEIN;

ARTICLE; BINDING SITE; CATALYST; CATALYTIC EFFICIENCY; CELL FUNCTION; CELL LYSATE; CONTROLLED STUDY; CYTOPLASM; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN LIGATION; PROTEIN MODIFICATION; STAPHYLOCOCCUS AUREUS; CHEMICAL STRUCTURE; CHEMISTRY; GENETICS; KINETICS; METABOLISM; MUTATION;

AMINOACYLTRANSFERASES; BACTERIAL PROTEINS; CALCIUM; CYSTEINE ENDOPEPTIDASES; CYTOPLASM; ESCHERICHIA COLI; KINETICS; MODELS, MOLECULAR; MUTATION; PROTEINS; STAPHYLOCOCCUS AUREUS;

EID: 84940614120     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201500012     Document Type: Article

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