Site-specific in situ growth of a cyclized protein-polymer conjugate with improved stability and tumor retention

Biomaterials

Volumn 47, Issue , 2015, Pages 13-19

  Hu, Jin ^a   Zhao, Wenguo ^a   Gao, Yan ^b   Sun, Mengmeng ^a   Wei, Yen ^b   Deng, Haiteng ^b   Gao, Weiping ^a  

^a Tsinghua University   (China)

^b TSINGHUA UNIVERSITY   (China)

Author keywords

Atom transfer radical polymerization; Protein delivery; Protein engineering; Protein polymer conjugate

Indexed keywords

AMINO ACIDS; CYCLIZATION; ETHYLENE; ETHYLENE GLYCOL; FREE RADICAL POLYMERIZATION; GENETIC ENGINEERING; PROTEINS; STABILITY; TUMORS;

CYSTEINE RESIDUES; GREEN FLUORESCENCE PROTEINS; OLIGO(ETHYLENE GLYCOL) METHYL ETHER METHACRYLATE; PROTEIN DELIVERY; PROTEIN ENGINEERING; PROTEIN-POLYMER CONJUGATES; RECOGNITION SEQUENCE; THERAPEUTIC PROTEIN;

ATOM TRANSFER RADICAL POLYMERIZATION;

CYCLOPEPTIDE; CYSTEINE; GLYCINE; GREEN FLUORESCENT PROTEIN; MALEIMIDE; POLYMER; POLY[OLIGO(ETHYLENE GLYCOL)METHYL ETHER METHACRYLATE]; SORTASE; UNCLASSIFIED DRUG; 2-(2-DIMETHYLAMINOETHYL)-5-BENZYLIDENE-6-METHYL-3-PYRIDAZINONE; AMINOACYLTRANSFERASE; BACTERIAL PROTEIN; BENZYL DERIVATIVE; BICINCHONINIC ACID; BIOMATERIAL; CYSTEINE PROTEINASE; PROTEIN; PROTEIN BINDING; PYRIDAZINE DERIVATIVE; QUINOLINE DERIVATIVE; SORTASE A;

AMINO TERMINAL SEQUENCE; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; ATOM TRANSFER RADICAL POLYMERIZATION; BIOTRANSFORMATION; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; CYCLIZATION; DRUG CONJUGATION; DRUG STABILITY; DRUG TUMOR LEVEL; ENZYME MECHANISM; FEMALE; GENETIC ENGINEERING; IN VITRO STUDY; IN VIVO STUDY; MOLECULAR RECOGNITION; MOUSE; NONHUMAN; PRIORITY JOURNAL; THERMOSTABILITY; TUMOR MODEL; ANIMAL; BAGG ALBINO MOUSE; CANCER TRANSPLANTATION; CHEMISTRY; CIRCULAR DICHROISM; LIQUID CHROMATOGRAPHY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ENGINEERING; PROTEIN TERTIARY STRUCTURE; SPECTROFLUOROMETRY; TANDEM MASS SPECTROMETRY; TUMOR CELL LINE;

AMINOACYLTRANSFERASES; ANIMALS; BACTERIAL PROTEINS; BENZYL COMPOUNDS; BIOCOMPATIBLE MATERIALS; CELL LINE, TUMOR; CHROMATOGRAPHY, LIQUID; CIRCULAR DICHROISM; CYSTEINE ENDOPEPTIDASES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FEMALE; GREEN FLUORESCENT PROTEINS; MICE; MICE, INBRED BALB C; NEOPLASM TRANSPLANTATION; POLYMERS; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN STRUCTURE, TERTIARY; PROTEINS; PYRIDAZINES; QUINOLINES; SPECTROMETRY, FLUORESCENCE; TANDEM MASS SPECTROMETRY;

EID: 84922817611     PISSN: 01429612     EISSN: 18785905     Source Type: Journal    
DOI: 10.1016/j.biomaterials.2015.01.002     Document Type: Article

References (13)

1. Abuchowski A., Vanes T., Palczuk N.C., Davis F.F. Alteration of immunological properties of bovine serum-albumin by covalent attachment of polyethylene-glycol. JBiol Chem 1977, 252:3578-3581.
2. Duncan R. The dawning era of polymer therapeutics. Nat Rev Drug Discov 2003, 2:347-360.
3. Alconcel S.N.S., Baas A.S., Maynard H.D. FDA-approved poly(ethylene glycol)-protein conjugate drugs. Polym Chem-Uk 2011, 2:1442-1448.
4. Lewis A., Tang Y.Q., Brocchini S., Choi J.W., Godwin A. Poly(2-methacryloyloxyethyl phosphorylcholine) for protein conjugation. Bioconjug Chem 2008, 19:2144-2155.
5. Keefe A.J., Jiang S.Y. Poly(zwitterionic)protein conjugates offer increased stability without sacrificing binding affinity or bioactivity. Nat Chem 2012, 4:60-64.
6. Mancini R.J., Lee J., Maynard H.D. Trehalose glycopolymers for stabilization of protein conjugates to environmental stressors. JAm Chem Soc 2012, 134:8474-8479.
7. Nguyen T.H., Kim S.H., Decker C.G., Wong D.Y., Loo J.A., Maynard H.D. Aheparin-mimicking polymer conjugate stabilizes basic fibroblast growth factor. Nat Chem 2013, 5:221-227.
8. Iwai H., Pluckthun A. Circular beta-lactamase: stability enhancement by cyclizing the backbone. Febs Lett 1999, 459:166-172.
9. Antos J.M., Popp M.W.L., Ernst R., Chew G.L., Spooner E., Ploegh H.L. Astraight path to circular proteins. JBiol Chem 2009, 284:16028-16036.
10. Popp M.W., Dougan S.K., Chuang T.Y., Spooner E., Ploegh H.L. Sortase-catalyzed transformations that improve the properties of cytokines. Proc Natl Acad Sci U S A 2011, 108:3169-3174.
11. Gao W.P., Liu W.G., Mackay J.A., Zalutsky M.R., Toone E.J., Chilkoti A. In situ growth of a stoichiometric PEG-like conjugate at a protein's N-terminus with significantly improved pharmacokinetics. Proc Natl Acad Sci U S A 2009, 106:15231-15236.
12. Gao W.P., Liu W.G., Christensen T., Zalutsky M.R., Chilkoti A. In situ growth of a PEG-like polymer from the C terminus of an intein fusion protein improves pharmacokinetics and tumor accumulation. Proc Natl Acad Sci U S A 2010, 107:16432-16437.
13. Qi Y.Z., Amiram M., Gao W.P., McCafferty D.G., Chilkoti A. Sortase-catalyzed initiator attachment enables high yield growth of a stealth polymer from the C terminus of a protein. Macromol Rapid Comm 2013, 34:1256-1260.