Engineered catalytic biofilms: Site-specific enzyme immobilization onto E. coli curli nanofibers

Biotechnology and Bioengineering

Volumn 112, Issue 10, 2015, Pages 2016-2024

  Botyanszki, Zsofia ^a,b   Tay, Pei Kun R ^b,c   Nguyen, Peter Q ^b,c   Nussbaumer, Martin G ^b,c   Joshi, Neel S ^b,c  

^a HARVARD UNIVERSITY   (United States)

^b HARVARD UNIVERSITY   (United States)

^c HARVARD UNIVERSITY   (United States)

Author keywords

Bacterial immobilization; Biocatalysis; Biofilm; Curli fibers; Enzyme display; Extracellular matrix

Indexed keywords

AMYLASES; BIOFILMS; CATALYSTS; CELL DEATH; COMPLEX NETWORKS; ENZYMES; ESCHERICHIA COLI; MOBILE SECURITY; NANOFIBERS; ORGANIC SOLVENTS; PURIFICATION;

BIOCATALYSIS; COMPLEX TRANSFORMATIONS; COVALENT IMMOBILIZATION; ENVIRONMENTAL CONDITIONS; EXTRACELLULAR MATRICES; EXTRACELLULAR POLYMERIC MATRIXES; FUNCTIONAL NANOFIBERS; IMMOBILIZATION TECHNIQUE;

ENZYME IMMOBILIZATION;

AMYLASE; NANOFIBER; BACTERIAL PROTEIN; CRL PROTEIN, BACTERIA; IMMOBILIZED ENZYME; SOLVENT;

ARTICLE; BACILLUS LICHENIFORMIS; BACTERIAL STRAIN; BIOCATALYSIS; BIOFILM; CELL METABOLISM; CELL PROLIFERATION; CELL VIABILITY; CONFOCAL MICROSCOPY; CONTROLLED STUDY; COVALENT BOND; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME PURIFICATION; ENZYME STABILITY; ESCHERICHIA COLI; EXTRACELLULAR MATRIX; IMMOBILIZED CELL; LIQUID CHROMATOGRAPHY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; SCANNING ELECTRON MICROSCOPY; TANDEM MASS SPECTROMETRY; CELL SURFACE DISPLAY; ENZYMOLOGY; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; PH; PHYSIOLOGY;

BACTERIA (MICROORGANISMS);

ALPHA-AMYLASES; BACTERIAL PROTEINS; BIOFILMS; CELL SURFACE DISPLAY TECHNIQUES; ENZYME STABILITY; ENZYMES, IMMOBILIZED; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; SOLVENTS;

EID: 84940467268     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25638     Document Type: Article

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