Characterization of the fecal microbiota of pigs before and after inoculation with "Brachyspira hampsonii"

PLoS ONE

Volumn 9, Issue 8, 2014, Pages

  Costa, Matheus O ^a   Chaban, Bonnie ^a   Harding, John C S ^a   Hill, Janet E ^a  

^a UNIVERSITY OF SASKATCHEWAN   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

RNA 16S; BACTERIAL DNA;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BACTERIAL GENE; BACTEROIDETES; BLOODY DIARRHEA; BRACHYSPIRA HAMPSONII INFECTION; CHEMOTAXONOMY; CONTROLLED STUDY; CPN60 GENE; DIARRHEA; FECES MICROFLORA; FIRMICUTES; GENE AMPLIFICATION; INOCULATION; MUCOHEMORRHAGIC DIARRHEA; NONHUMAN; SEQUENCE ANALYSIS; SPECIES DIVERSITY; SPECIES RICHNESS; SPIROCHETE INFECTION; SWINE; ANIMAL; BRACHYSPIRA; CLASSIFICATION; COMPARATIVE STUDY; FECES; GASTROINTESTINAL HEMORRHAGE; GRAM NEGATIVE INFECTION; GRAM POSITIVE BACTERIUM; ISOLATION AND PURIFICATION; MICROBIOLOGY; MICROFLORA; PHYSIOLOGY; PIG; SWINE DISEASE; VETERINARY;

ANIMALS; BRACHYSPIRA; DIARRHEA; DNA, BACTERIAL; FECES; GASTROINTESTINAL HEMORRHAGE; GRAM-NEGATIVE BACTERIAL INFECTIONS; GRAM-POSITIVE BACTERIA; MICROBIOTA; SWINE; SWINE DISEASES;

EID: 84940306819     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0106399     Document Type: Article

References (25)

1. Chander Y, Primus A, Oliveira S, Gebhart CJ (2012) Phenotypic and molecular characterization of a novel strongly hemolytic Brachyspira species, provisionally designated "Brachyspira hampsonii". J Vet Diagnost Invest 24: 903-910.
2. Burrough ER, Strait EL, Kinyon JM, Bower LP, Madson DM, et al. (2012) Comparative virulence of clinical Brachyspira spp. isolates in inoculated pigs. J Vet Diagnost Invest 24: 1025-1034.
3. Rubin JE, Costa MO, Hill JE, Kittrell HE, Fernando C, et al. (2013) Reproduction of mucohaemorrhagic diarrhea and colitis indistinguishable from swine dysentery following experimental inoculation with "Brachyspira hampsonii" strain 30446. PLoS ONE 8: e57146.
4. Patterson AH, Rubin JE, Fernando C, Costa MO, Harding JC, et al. (2013) Fecal shedding of Brachyspira spp. on a farrow-to-finish swine farm with a clinical history of "Brachyspira hampsonii"-associated colitis. BMC Vet Res 9: 137.
5. Harris DL, Alexander TJL, Whipp SC, Robinson IM, Glock RD, et al. (1978) Swine dysentery: studies of gnotobiotic pigs inoculated with Treponema hyodysenteriae, Bacteroides vulgatus, and Fusobacterium necrophorum . J Am Vet Med Assoc 172: 468-471. (Pubitemid 8293709)
6. Meyer RC, Simon J, Byerly CS (1975) The etiology of swine dysentery. III. The role of selected gram-negative obligate anaerobes. Vet Pathol 12: 46-54.
7. Meyer RC, Simon J, Byerly CS (1974) The etiology of swine dysentery. II. Effect of a known microbial flora, weaning and diet on disease production in gnotobiotic and conventional swine. Vet Pathol 11: 527-534.
8. Whipp SC, Robinson IM, Harris DL, Glock RD, Matthews PJ, et al. (1979) Pathogenic synergism between Treponema hyodysenteriae and other selected anaerobes in gnotobiotic pigs. Infect Immun 26: 1042-1047. (Pubitemid 10212965)
9. Alvarez-Ordonez A, Martinez-Lobo FJ, Arguello H, Carvajal A, Rubio P (2013) Swine dysentery: aetiology, pathogenicity, determinants of transmission and the fight against the disease. Int J Env Res Pub Health 10: 1927-1947.
10. Leser TD, Lindecrona RH, Jensen TK, Jensen BB, Moller K (2000) Changes in bacterial community structure in the colon of pigs fed different experimental diets and after infection with Brachyspira hyodysenteriae . Appl Environ Microbiol 66: 3290-3296. (Pubitemid 30624570)
11. Robinson IM, Whipp SC, Bucklin JA, Allison MJ (1984) Characterization of predominant bacteria from the colons of normal and dysenteric pigs. Appl Environ Microbiol 48: 964-969. (Pubitemid 15205871)
12. Harris DL, Hampson DJ, Glock RD (1999) Swine Dysentery. In: Straw BE, D'Allaire S, Mengeling WL, Taylor DJ, editors. Diseases of Swine. 8th ed. Ames, Iowa, USA: Iowa State University Press. 579-600.
13. Schellenberg J, Links MG, Hill JE, Hemmingsen SM, Peters GA, et al. (2011) Pyrosequencing of chaperonin-60 (cpn60) amplicons as a means of determining microbial community composition. Methods Mol Biol 733: 143-158.
14. Lee DH, Zo YG, Kim SJ (1996) Nonradioactive method to study genetic profiles of natural bacterial communities by PCR-single-strand-conformation polymorphism. Appl Environ Microbiol 62: 3112-3120.
15. Chaban B, Ngeleka M, Hill JE (2010) Detection and quantification of 14 Campylobacter species in pet dogs reveals an increase in species richness in feces of diarrheic animals. BMC Microbiol 10: 73.
16. Links MG, Chaban B, Hemmingsen SM, Muirhead K, Hill JE (2013) mPUMA: a computational approach to microbiota analysis by de novo assembly of OTUs based on protein-coding barcode sequences. Microbiome 1: 23.
17. Grabherr MG, Haas BJ, Yassour M, Levin JZ, Thompson DA, et al. (2011) Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat Biotechnol 29: 644-652.
18. Langmead B, Salzberg SL (2012) Fast gapped-read alignment with Bowtie 2. Nature Meth 9: 357-359.
19. Hill JE, Penny SL, Crowell KG, Goh SH, Hemmingsen SM (2004) cpnDB: a chaperonin sequence database. Genome Res 14: 1669-1675. (Pubitemid 39193418)
20. Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, et al. (2009) Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol 75: 7537-7541.
21. Gihring TM, Green SJ, Schadt CW (2012) Massively parallel rRNA gene sequencing exacerbates the potential for biased community diversity comparisons due to variable library sizes. Environ Microbiol 14: 285-290.
22. Kim HB, Borewicz K, White BA, Singer RS, Sreevatsan S, et al. (2011) Longitudinal investigation of the age-related bacterial diversity in the feces of commercial pigs. Vet Microbiol 153: 124-133.
23. Hill JE, Seipp RP, Betts M, Hawkins L, Van Kessel AG, et al. (2002) Extensive profiling of a complex microbial community by high-throughput sequencing. Appl Environ Microbiol 68: 3055-3066. (Pubitemid 34602031)
24. Chaban B, Links MG, Hill JE (2012) A molecular enrichment strategy based on cpn60 for detection of Epsilon-Proteobacteria in the dog fecal microbiome. Microbial Ecol 63: 348-357.
25. Bishara J, Farah R, Mograbi J, Khalaila W, Abu-Elheja O, et al. (2013) Obesity as a risk factor for Clostridium difficile infection. Clin Infect Dis 57: 489-493.