Ezrin-related Phosphoinositide pathway modifies RhoA and Rac1 in human osteosarcoma cell lines

Journal of Cell Communication and Signaling

Volumn 9, Issue 1, 2015, Pages 55-62

  Lo Vasco, V R ^a   Leopizzi, M ^a   Della Rocca, C ^a  

^a SAPIENZA UNIVERSITY OF ROME   (Italy)

Author keywords

Ezrin; Osteosarcoma; Phospholipase C; Rac1; Ras GTPases; RhoA

Indexed keywords

EID: 84939958665     PISSN: 18739601     EISSN: 1873961X     Source Type: Journal    
DOI: 10.1007/s12079-015-0265-y     Document Type: Article

References (46)

1. Auvinen E, Kivi N, Vaheri A (2007) Regulation of ezrin localization by Rac1 and PIPK in human epithelial cells. Exp Cell Res 313:824–833
2. Barret C, Roy C, Montcourrier P, Mangeat P, Niggli V (2000) Mutagenesis of the phosphatidylinositol 4,5-bisphosphate (PIP(2)) binding site in the NH(2)-terminal domain of ezrin correlates with its altered cellular distribution. J Cell Biol 151(5):1067–1080
3. Benitah SA, Valeron PF, Van AL, Marshall CJ, Lacal JC (2004) Rho GTPases in human cancer: an unresolved link to upstream and downstream transcriptional regulation. Biochim Biophys Acta 1705:121–132
4. Berridge MJ (1993) Inositol trisphosphate and calcium signalling. Nature 361:315–325
5. Berridge MJ, Dupont G (1994) Spatial and temporal signalling by calcium. Curr Opin Cell Biol 6(2):267–274
6. Bunney TD, Katan M (2011) PLC regulation: emerging pictures for molecular mechanisms. Trends Biochem Sci 36(2):88–96
7. De Smedt H, Parys JB (1995) Molecular and functional diversity of inositol triphosphate-induced Ca(2+) release. Verh K Acad Geneeskd Belg 57:423–458
8. Defacque H, Egeberg M, Habermann A, Diakonova M, Roy C, Mangeat P, Voelter W, Marriott G, Pfannstiel J, Faulstich H, Griffiths G (2000) Involvement of ezrin/moesin in de novo actin assembly on phagosomal membranes. EMBO J 19(2):199–212
9. Defacque H, Bos E, Garvalov B, Barret C, Roy C, Mangeat P, Shin HW, Rybin V, Griffiths G (2002) Phosphoinositides regulate membrane-dependent actin assembly by latex bead phagosomes. Mol Biol Cell 13(4):1190–1202
10. Divecha N, Irvine RF (1995) Phospholipid signaling. Cell 80(2):269–278
11. Ferrari S, Zanella L, Alberghini M et al (2008) Prognostic significance of immunohistochemical expression of ezrin in non-metastatic high-grade osteosarcoma. Pediatr Blood Cancer 50:752–756
12. Fievet BT, Gautreau A, Roy C, Del Maestro L, Mangeat P, Louvard D, Arpin M (2004) Phosphoinositide binding and phosphorylation act sequentially in the activation mechanism of ezrin. J Cell Biol 164:653–659
13. Fievet B, Louvard D, Arpin M (2007) ERM proteins in epithelial cell organization and functions. Biochim Biophys Acta 1773:653–660
14. Fukami K, Inanobe S, Kanemaru K, Nakamura Y (2010) Phospholipase C is a key enzyme regulating intracellular calcium and modulating the phosphoinositide balance. Prog Lipid Res 49(4):429–437
15. Gatta G, Capocaccia R, Stiller C, Kaatsch P, Berrino F, Terenziani M (2005) EUROCARE Working Group. Childhood cancer survival trends in Europe: a EUROCARE Working Group study. J Clin Oncol 3(16):3742–3751
16. Gómez del Pulgar T, Benitah SA, Valerón PF, Espina C, Lacal JC (2005) Rho GTPase expression in tumourigenesis: evidence for a significant link. Bioessays 27(6):602–613
17. Hao JJ, Liu Y, Kruhlak M, Debell KE, Rellahan BL, Shaw S (2009) Phospholipase C-mediated hydrolysis of PIP2 releases ERM proteins from lymphocyte membrane. J Cell Biol 184(3):451–462
18. Hartwig JH, Bokoch GM, Carpenter CL, Janmey PA, Taylor LA, Toker A, Stossel TP (1995) Thrombin receptor ligation and activated Rac uncap actin filament barbed ends through phosphoinositide synthesis in permeabilized human platelets. Cell 82(4):643–653
19. Hisatsune C, Nakamura K, Kuroda Y et al (2005) Amplification of Ca2+ signaling by diacylglycerolmediatedinositol 1,4,5-trisphosphate production. J Biol Chem 280(12):11723–11730
20. Honda A, Nogami M, Yokozeki T, Yamazaki M, Nakamura H, Watanabe H, Kawamoto K, Nakayama K, Morris AJ, Frohman MA, Kanaho Y (1999) Phosphatidylinositol 4-phosphate 5-kinase alpha is a downstream effector of the small G protein ARF6 in membrane ruffle formation. Cell 99(5):521–532
21. Irvine RF (2003) Nuclear lipid signalling. Nat Rev Mol Cell Biol 4(5):349–360
22. Joneson T, Bar-Sagi D (1997) Ras effectors and their role in mitogenesis and oncogenesis. J Mol Med (Berl) 75(8):587–593
23. Juncadella IJ, Kadl A, Sharma AK, Shim YM, Hochreiter-Hufford A, Borish L, Ravichandran KS (2013) Apoptotic cell clearance by bronchial epithelial cells critically influences airway inflammation. Nature 493(7433):547–551
24. Kaminskas E, Farrell A, Abraham S, Baird A, Hsieh LS, Lee SL, Leighton JK, Patel H, Rahman A, Sridhara R, Wang YC, Pazdur R, FDA (2005) Approval summary: azacitidine for treatment of myelodysplastic syndrome subtypes. Clin Cancer Res 11(10):3604–3608
25. Khanna C, Wan X, Bose S et al (2004) The membrane-cytoskeleton linker ezrin is necessary for osteosarcoma metastasis. Nat Med 10:182–186
26. Li W, Chong H, Guan KL (2001) Function of the Rho family GTPases in Ras-stimulated Raf activation. J Biol Chem 276(37):34728–34737
27. Li Y, Chen Y, Xu J (2011) Factors influencing RhoA protein distribution in the nucleus. Mol Med Rep 4(6):1115–1119
28. Lo Vasco VR (2010) Signaling in the genomic era. J Cell Commun Signal 4(3):115–117
29. Lo Vasco VR, Calabrese G, Manzoli L, Palka GD, Spadano A, Morizio E, Guanciali-Franchi P, Fantasia D, Cocco L (2004) Inositide-specific Phospholipase C β1 gene deletion in the progression of Myelodisplastic Syndrome to Acute Myeloid Leukemia. Leukemia 18(6):1122–1126
30. Lo Vasco VR, Leopizzi M, Chiappetta C, Businaro R, Polonia P, Della Rocca C, Litta P (2012) Expression of Phosphoinositide-specific Phospholipase C enzymes in normal endometrium and in endometriosis. Fertil Steril 98(2):410–414
31. Lo Vasco VR, Leopizzi M, Puggioni C, Della RC (2013a) Ezrin silencing remodulates the expression of Phosphoinositide-specific Phospholipase C signal transduction pathway in human osteosarcoma cell lines. J Cell Commun Signal 7(2):141–150
32. Lo Vasco VR, Leopizzi M, Chiappetta C, Puggioni C, Della Rocca C, Businaro R (2013b) Lypopolysaccharide down-regulates the expression of selected phospholipase C genes in cultured endothelial cells. Inflammation 36(4):862–868
33. Lo Vasco VR, Leopizzi M, Puggioni C, Della Rocca C, Businaro R (2014a) Fibroblast growth factor acts upon the transcription of phospholipase C genes in human umbilical vein endothelial cells. Mol Cell Biochem 388(1):51–59
34. Lo Vasco VR, Leopizzi M, Stoppoloni D, Della RC (2014b) Silencing of Phosphoinositide-specific Phospholipase C ε enzyme remodulates the expression of Phosphoinositide signal transduction pathway in human osteosarcoma cell lines. Anticancer Res 34(8):4069–4075
35. Lo Vasco VR, Leopizzi M, Puggioni C, Della RC (2014c) Ezrin silencing remodulates the expression of Phosphoinositide-specific Phospholipase C signal transduction pathway in human osteosarcoma cell lines. J Cell Commun Signal 8(3):219–229
36. Martins M, McCarthy A, Baxendale R, Guichard S, Magno L, Kessaris N, El-Bahrawy M, Yu P, Katan M (2014) Tumor suppressor role of phospholipase C{varepsilon} in Ras-triggered cancers. Proc Natl Acad Sci USA. Mar 3 (epub)
37. Mirabello L, Troisi RJ, Savage SA (2009) International osteosarcoma incidence patterns in children and adolescents, middle ages and elderly persons. Int J Cancer 125:229–234
38. Moore KA, Sethi R, Doanes AM, Johnson TM, Pracyk JB, Kirby M, Irani K, Goldschmidt-Clermont PJ, Finkel T (1997) Rac1 is required for cell proliferation and G2/M progression. Biochem J 326(Pt 1):17–20
39. Nishizuka Y (1995) Protein kinase C and lipid signaling for sustained cellular responses. FASEB J 9:484–496
40. Rhee SG (2001) Regulation of phosphoinositide-specific phospholipase C. Annu Rev Biochem 70:281–312
41. Rhee SG, Kim H, Suh PG, Choi WC (1991) Multiple forms of phosphoinositide-specific phospholipase C and different modes of activation. Biochem Soc Trans 19(2):337–341
42. Ridley AJ (2001) Rho family proteins: coordinating cell responses. Trends Cell Biol 11:471–477
43. Snyder JT, Singer AU, Wing MR, Harden TK, Sondek J (2003) The Pleckstrin Homology Domain of Phospholipase C-β2 as an Effector Site for Rac. J Biol Chem 278:21099–21104
44. Steffen A, Rottner K, Ehinger J, Innocenti M, Scita G, Wehland J, Stradal TE (2004) Sra-1 and Nap1 link Rac to actin assembly driving lamellipodia formation. EMBO J 23(4):749–759
45. Suh PG, Park J, Manzoli L, Cocco L, Peak JC, Katan M, Fukami K, Kataoka T, Yuk S, Ryu SH (2008) Multiple roles of phosphoinositide-specific phospholipase C isozymes. BMB Rep 41:415–434
46. Tolias KF, Hartwig JH, Ishihara H, Shibasaki Y, Cantley LC, Carpenter CL (2000) Type Ialpha phosphatidylinositol-4-phosphate 5-kinase mediates Rac-dependent actin assembly. Curr Biol 10(3):153–156