Phosphoinositide binding and phosphorylation act sequentially in the activation mechanism of ezrin

Journal of Cell Biology

Volumn 164, Issue 5, 2004, Pages 653-659

  Fievet, Bruno T ^a   Gautreau, Alexis ^a   Roy, Christian ^b   Del Maestro, Laurence ^a   Mangeat, Paul ^b   Louvard, Daniel ^a   Arpin, Monique ^a,c  

^a INSTITUT CURIE   (France)

^b UNIV MONTPELLIER   (France)

^c INSTITUT CURIE   (France)

Author keywords

Actin cytoskeleton; Epithelial cell morphogenesis; ERM proteins; PIP2

Indexed keywords

ACTIN; EZRIN; PHOSPHATIDYLINOSITIDE; PHOSPHATIDYLINOSITOL 4,5 BISPHOSPHATE; THREONINE;

ACTIN FILAMENT; AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; BINDING SITE; CELL FUNCTION; CELL STRUCTURE; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; CYTOSKELETON; EPITHELIUM CELL; GENE MUTATION; IN VIVO STUDY; MEMBRANE BINDING; MOLECULAR INTERACTION; MOUSE; NONHUMAN; PHOSPHOINOSITIDE METABOLISM; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; REGULATORY MECHANISM;

ANIMALS; BINDING SITES; CELL LINE; CELL MEMBRANE; CELL POLARITY; CELL SIZE; CYTOSKELETAL PROTEINS; EPITHELIAL CELLS; MORPHOGENESIS; PHOSPHATIDYLINOSITOL 4,5-DIPHOSPHATE; PHOSPHATIDYLINOSITOLS; PHOSPHOPROTEINS; PHOSPHORYLATION; POINT MUTATION; PROTEIN BINDING; THREONINE;

ANIMALIA;

EID: 1442285361     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.200307032     Document Type: Article

References (26)

1. Algrain, M., O. Turunen, A. Vaheri, D. Louvard, and M. Arpin. 1993. Ezrin contains cytoskeleton and membrane binding domains accounting for its proposed role as a membrane-cytoskeletal linker. J. Cell Biol. 120:129-139.
2. 2-terminal domain of ezrin correlates with its altered cellular distribution. J. Cell Biol. 151:1067-1079.
3. Bretscher, A., K. Edwards, and R.G. Fehon. 2002. ERM proteins and merlin: integrators at the cell cortex. Nat. Rev. Mol. Cell Biol. 3:586-599.
4. Gary, R., and A. Bretscher. 1995. Ezrin self-association involves binding of an N-terminal domain to a normally masked C-terminal domain that includes the F-actin binding site. Mol. Biol. Cell. 6:1061-1075.
5. Gautreau, A., D. Louvard, and M. Arpin. 2000. Morphogenic effects of ezrin require a phosphorylation-induced transition from oligomers to monomers at the plasma membrane. J. Cell Biol. 150:193-203.
6. Gautreau, A., D. Louvard, and M. Arpin. 2002. ERM proteins and NF2 tumor suppressor: the Yin and Yang of cortical actin organization and cell growth signaling. Curr. Opin. Cell Biol. 14:104-109.
7. Hamada, K., T. Shimizu, T. Matsui, S. Tsukita, S. Tsukita, and T. Hakoshima. 2000. Structural basis of the membrane-targeting and unmasking mechanisms of the radixin FERM domain. EMBO J. 19:4449-4462.
8. Hayashi, K., S. Yonemura, T. Matsui, and S. Tsukita. 1999. Immunofluorescence detection of ezrin/radixin/moesin (ERM) proteins with their carboxyl-terminal threonine phosphorylated in cultured cells and tissues. J. Cell Sci. 112:1149-1158.
9. Heiska, L., K. Alfthan, M. Grönholm, P. Vilja, A. Vaheri, and O. Carpén. 1998. Association of ezrin with intercellular adhesion molecule-1 and -2 (ICAM-1 and ICAM-2). J. Biol. Chem. 273:21893-21900.
10. Hirao, M., N. Sato, T. Kondo, S. Yonemura, M. Monden, T. Sasaki, Y. Takai, S. Tsukita, and S. Tsukita. 1996. Regulation mechanism of ERM (ezrin/radixin/moesin) protein/plasma membrane association: possible involvement of phosphatidylinositol turnover and rho-dependent signaling pathway. J. Cell Biol. 135:37-51.
11. Kreis, T.E. 1986. Microinjected antibodies against the cytoplasmic domain of vesicular stomatitis virus glycoprotein block its transport to the cell surface. EMBO J. 5:931-941.
12. Magendantz, M., M.D. Henry, A. Lander, and F. Solomon. 1995. Interdomain interactions of radixin in vitro. J. Biol. Chem. 270:25324-25327.
13. Matsui, T., M. Maeda, Y. Doi, S. Yonemura, M. Amano, K. Kaibuchi, S. Tsukita, and S. Tsukita. 1998. Rho-kinase phosphorylates COOH-terminal threonines of ezrin/radixin/moesin (ERM) proteins and regulates their head-to-tail association. J. Cell Biol. 140:647-657.
14. Nakamura, F., M.R. Amieva, C. Hirota, Y. Mizuno, and H. Furthmayr. 1996. Phosphorylation of T-558 of moesin detected by site-specific antibodies in RAW264.7 macrophages. Biochem. Biophys. Res. Commun. 226:650-656.
15. Nakamura, F., L. Huang, K. Pestonjamasp, E. J. Luna, and H. Furthmayr. 1999. Regulation of F-actin binding to platelet moesin in vitro by both phosphorylation of threonine 558 and polyphosphatidylinositides. Mol. Biol. Cell. 10:2669-2685.
16. Niggli, V., C. Andréoli, C. Roy, and P. Mangeat. 1995. Identification of a phosphatidylinositol-4, 5-biphosphate-binding domain in the N-terminal region of ezrin. FEBS Lett. 376:172-176.
17. Oshiro, N., Y. Fukata, and K. Kaibuchi. 1998. Phosphorylation of moesin by Rho-associated kinase (Rho-kinase) plays a crucial role in the formation of microvilli-like structures. J. Biol. Chem. 273:34663-34666.
18. Pearson, M.A., D. Reczek, A. Bretscher, and P.A. Karplus. 2000. Structure of the ERM protein moesin reveals the FERM domain fold masked by an extended actin binding tail domain. Cell. 101:259-270.
19. Polesello, C., I. Delon, P. Valenti, P. Ferrer, and F. Payre. 2002. Dmoesin controls actin-based cell shape and polarity during Drosophila melanogaster oogenesis. Nat. Cell Biol. 4:782-789.
20. Pujuguet, P., L. Del Maestro, A. Gautreau, D. Louvard, and M. Arpin. 2003. Ezrin regulates E-cadherin-dependent adherens junction assembly through Rac1 activation. Mol. Biol. Cell. 14:2181-2191.
21. Simons, P.C., S.F. Pietromonaco, D. Reczek, A. Bretscher, and L. Elias. 1998. C-terminal threonine phosphorylation activates ERM proteins to link the cell's cortical lipid bilayer to the cytoskeleton. Biochem. Biophys. Res. Commun. 253:561-565.
22. Speck, O., S.C. Hughes, N.K. Noren, R.M. Kulikauskas, and R.G. Fehon. 2003. Moesin functions antagonistically to the Rho pathway to maintain epithelial integrity. Nature. 421:83-87.
23. Turunen, O., T. Wahlström, and A. Vaheri. 1994. Ezrin has a COOH-terminal actin-binding site that is conserved in the ezrin protein family. J. Cell Biol. 126:1445-1453.
24. 3H]inositol-labeled phosphoinositide pools. J. Cell Biol. 143:501-510.
25. Yonemura, S., S. Tsukita, and S. Tsukita. 1999. Direct involvement of ezrin/radixin/moesin (ERM)-binding membrane proteins in the organization of microvilli in collaboration with activated proteins. J. Cell Biol. 145:1497-1509.
26. Yonemura, S., T. Matsui, S. Tsukita, and S. Tsukita. 2002. Rho-dependent and -independent activation mechanisms of ezrin/radixin/moesin proteins: an essential role for polyphosphoinositides in vivo. J. Cell Sci. 115:2569-2580.