High-level secretory expression and purification of unhydroxylated human collagen α1(III) chain in Pichia pastoris GS115

Biotechnology and Applied Biochemistry

Volumn 62, Issue 4, 2015, Pages 467-475

  Li, Linbo ^a   Fan, Daidi ^a   Ma, Xiaoxuan ^a   Deng, Jianjun ^a   He, Jing ^a  

^a NORTHWEST UNIVERSITY   (China)

Author keywords

Pichia pastoris; purification; rhCOL3A1; secretory expression

Indexed keywords

BIOLOGICAL ORGANS; BIOMEDICAL ENGINEERING; CHAINS; CLONING; COLLAGEN; ELECTROPHORESIS; PURIFICATION; SODIUM DODECYL SULFATE; SODIUM SULFATE; YEAST;

GENETICALLY ENGINEERED MICROORGANISMS; METHYLOTROPHIC YEASTS; PICHIA PASTORIS; PICHIA PASTORIS GS115; RECOMBINANT COLLAGENS; RHCOL3A1; SECRETORY EXPRESSION; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS;

GENE EXPRESSION;

AMINO TERMINAL TELOPEPTIDE; CARBOXY TERMINAL TELOPEPTIDE; COLLAGEN; COLLAGEN ALPHA 1; COMPLEMENTARY DNA; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; COL3A1 PROTEIN, HUMAN; COLLAGEN TYPE 3;

ARTICLE; BHK CELL LINE; CELL PROLIFERATION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CULTURE MEDIUM; EXPRESSION VECTOR; KOMAGATAELLA PASTORIS; LARGE SCALE PRODUCTION; METHYLOTROPHIC YEAST; MOLECULAR CLONING; MOLECULAR WEIGHT; MTT ASSAY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SECRETION; SUPERNATANT; ANIMAL; BIOSYNTHESIS; CELL LINE; CHEMISTRY; DRUG EFFECTS; GENE EXPRESSION; GENE VECTOR; GENETICS; HAMSTER; HUMAN; METABOLISM; PICHIA;

CRICETINAE; PICHIA PASTORIS;

ANIMALS; CELL LINE; CELL PROLIFERATION; CLONING, MOLECULAR; COLLAGEN TYPE III; CRICETINAE; GENE EXPRESSION; GENETIC VECTORS; HUMANS; PICHIA; RECOMBINANT PROTEINS;

EID: 84939563009     PISSN: 08854513     EISSN: 14708744     Source Type: Journal    
DOI: 10.1002/bab.1297     Document Type: Article

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