Long term intravital multiphoton microscopy imaging of immune cells in healthy and diseased liver using CXCR6.Gfp reporter mice

Journal of Visualized Experiments

Volumn 2015, Issue 97, 2015, Pages

  Heymann, Felix ^a   Niemietz, Patricia M ^a   Peusquens, Julia ^a   Ergen, Can ^a   Kohlhepp, Marlene ^a   Mossanen, Jana C ^a   Schneider, Carlo ^a   Vogt, Michael ^a   Tolba, Rene H ^b   Trautwein, Christian ^a   Martin, Christian ^a   Tacke, Frank ^a  

^a UNIVERSITY HOSPITAL RWTH AACHEN   (Germany)

^b RWTH AACHEN UNIVERSITY   (Germany)

Author keywords

Immunology; Inflammation; Intravital imaging; Issue 97; Leukocyte traffic; Liver; Microscopy; Migration; TPLSM; Two photon microscopy

Indexed keywords

CHEMOKINE RECEPTOR; CHEMOKINE RECEPTOR CXCR; CXCR6 PROTEIN, MOUSE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN;

ANIMAL; B LYMPHOCYTE; C57BL MOUSE; CELL COMMUNICATION; CELL MOTION; CHEMISTRY; CYTOLOGY; GENETICS; IMMUNOLOGY; LIVER; LIVER DISEASE; MOUSE; MULTIPHOTON MICROSCOPY; NATURAL KILLER T CELL; PATHOLOGY; PROCEDURES; T LYMPHOCYTE SUBPOPULATION; TRANSGENIC MOUSE;

ANIMALS; B-LYMPHOCYTES; CELL COMMUNICATION; CELL MOVEMENT; GREEN FLUORESCENT PROTEINS; LIVER; LIVER DISEASES; MICE; MICE, INBRED C57BL; MICE, TRANSGENIC; MICROSCOPY, FLUORESCENCE, MULTIPHOTON; NATURAL KILLER T-CELLS; RECEPTORS, CHEMOKINE; RECEPTORS, CXCR; RECOMBINANT FUSION PROTEINS; T-LYMPHOCYTE SUBSETS;

EID: 84939292424     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/52607     Document Type: Article

References (40)

1. Dusseaux, M., et al.Human MAIT cells are xenobiotic-resistant, tissue-targeted, CD161hi IL-17-secreting T cells. Blood. 117, (4), 1250-1259 (2011).
2. Reese, A. J. The effect of hypoxia on liver secretion studied by intravital fluorescence microscopy. Br J Exp Pathol. 41, 527-535 (1960).
3. Bhathal, P. S., Christie, G. S. Intravital fluorescence microscopy study of bile ductule proliferation in guinea pigs. Gut. 10, (11), 955 (1969).
4. Stefenelli, N. Terminal vascular system and microcirculation of the rat liver in intravital microscopy. Wien Klin Wochenschr. 82, (33), 575-578 (1970).
5. Hori, T., et al.Simple and sure methodology for massive hepatectomy in the mouse. Ann Gastroenterol. 24, (4), 307-318 (2011).
6. Tanaka, K., et al.Intravital dual-colored visualization of colorectal liver metastasis in living mice using two photon laser scanning microscopy. Microsc Res Tech.75, (3), 307-315 (2011).
7. Schemmer, P., Bunzendahl, H., Klar, E., Thurman, R. G. Reperfusion injury is dramatically increased by gentle liver manipulation during harvest. Transpl Int. 13, Suppl 1. S525-S527 (2000).
8. Schemmer, P., et al.Activated Kupffer cells cause a hypermetabolic state after gentle in situ manipulation of liver in rats. Am J Physiol Gastrointest Liver Physiol. 280, (6), G1076-G1082 (2001).
9. Toiyama, Y., et al.Intravital imaging of DSS-induced cecal mucosal damage in GFP-transgenic mice using two-photon microscopy. J Gastroenterol.45, (5), 544-553 (2010).
10. Zimmon, D. S. The hepatic vasculature and its response to hepatic injury: a working hypothesis. Yale J Biol Med. 50, (5), 497-506 (1977).
11. Wong, J., et al.A minimal role for selectins in the recruitment of leukocytes into the inflamed liver microvasculature. J Clin Invest.99, (11), 2782-2790 (1997).
12. Bonder, C. S., et al.Essential role for neutrophil recruitment to the liver in concanavalin A-induced hepatitis. J Immunol. 172, (1), 45-53 (2004).
13. Xu, C., Zipfel, W., Shear, J. B., Williams, R. M., Webb, W. W. Multiphoton fluorescence excitation: new spectral windows for biological nonlinear microscopy. Proc Natl Acad Sci U S A. 93, (20), 10763-10768 (1996).
14. Centonze, V. E., White, J. G. Multiphoton excitation provides optical sections from deeper within scattering specimens than confocal imaging. Biophys J. 75, (4), 2015-2024 (1998).
15. Amore, J. D., et al. In vivo multiphoton imaging of a transgenic mouse model of Alzheimer disease reveals marked thioflavine-S-associated alterations in neurite trajectories. J Neuropathol Exp Neurol. 62, (2), 137-145 (2003).
16. Hickey, M. J., Westhorpe, C. L. V. Imaging inflammatory leukocyte recruitment in kidney, lung and liver--challenges to the multi-step paradigm. Immunol Cell Biol. 91, (4), 281-289 (2013).
17. McLellan, M. E., Kajdasz, S. T., Hyman, B. T., Bacskai, B. J. In vivo imaging of reactive oxygen species specifically associated with thioflavine S-positive amyloid plaques by multiphoton microscopy. J Neurosci.23, (6), 2212-2217 (2003).
18. Geissmann, F., et al.Intravascular Immune Surveillance by CXCR6+ NKT Cells Patrolling Liver Sinusoids. PLoS Biology. 3, (4), (2005).
19. Velázquez, P., et al.Cutting edge: activation by innate cytokines or microbial antigens can cause arrest of natural killer T cell patrolling of liver sinusoids. J Immunol. 180, (4), 2024-2028 (2008).
20. Wehr, A., et al.Chemokine receptor CXCR6-dependent hepatic NK T Cell accumulation promotes inflammation and liver fibrosis. J Immunol. 190, (10), 5226-5236 (2013).
21. Khandoga, A., Hanschen, M., Kessler, J. S., Krombach, F. CD4+ T cells contribute to postischemic liver injury in mice by interacting with sinusoidal endothelium and platelets. Hepatology. 43, (2), 306-315 (2006).
22. Egen, J. G., et al.Macrophage and T cell dynamics during the development and disintegration of mycobacterial granulomas. Immunity. 28, (2), 271-284 (2008).
23. Beattie, L., et al.Leishmania donovani-induced expression of signal regulatory protein alpha on Kupffer cells enhances hepatic invariant NKTcell activation. Eur J Immunol. 40, (1), 117-123 (2010).
24. Beattie, L., et al.Dynamic imaging of experimental Leishmania donovani-induced hepatic granulomas detects Kupffer cell-restricted antigen presentation to antigen-specific CD8 T cells. PLoS Pathog. 6, (3), e1000805 (2010).
25. McDonald, B., et al.Intravascular danger signals guide neutrophils to sites of sterile inflammation. Science. 330, (6002), 362-366 (2010).
26. Vanheule, E., et al.An intravital microscopic study of the hepatic microcirculation in cirrhotic mice models: relationship between fibrosis and angiogenesis. Int J Exp Pathol. 89, (6), 419-432 (2008).
27. Jenne, C. N., Kubes, P. Immune surveillance by the liver. Nat Immunol.14, (10), 996-1006 (2013).
28. Zimmermann, H. W., Tacke, F. Modification of chemokine pathways and immune cell infiltration as a novel therapeutic approach in liver inflammation and fibrosis. Inflamm Allergy Drug Targets. 10, (6), 509-536 (2011).
29. Kim, J. V., et al.Two-photon laser scanning microscopy imaging of intact spinal cord and cerebral cortex reveals requirement for CXCR6 and neuroinflammation in immune cell infiltration of cortical injury sites. J Immunol Methods. 352, (1-2), 89-100 (2010).
30. Karlmark, K. R., et al.Hepatic recruitment of the inflammatory Gr1+ monocyte subset upon liver injury promotes hepatic fibrosis. Hepatology. 50, (1), 261-274 (2009).
31. Heymann, F., et al.Hepatic macrophage migration and differentiation critical for liver fibrosis is mediated by the chemokine receptor C-C motif chemokine receptor 8 in mice. Hepatology. 55, (3), 898-909 (2012).
32. Ramachandran, P., et al.Differential Ly-6C expression identifies the recruited macrophage phenotype, which orchestrates the regression of murine liver fibrosis. Proc Natl Acad Sci U S A. 109, (46), E3186-E3195 (2012).
33. Moles, A., et al.A TLR2/S100A9/CXCL-2 signaling network is necessary for neutrophil recruitment in acute and chronic liver injury in the mouse. J Hepatol. 60, (4), 782-791 (2014).
34. Hammerich, L., et al.Chemokine receptor CCR6-dependent accumulation of γδ T cells in injured liver restricts hepatic inflammation and fibrosis. Hepatology. 59, (2), 630-642 (2014).
35. Syn, W. -K., et al.NKT-associated hedgehog and osteopontin drive fibrogenesis in non-alcoholic fatty liver disease. Gut. 61, (9), 1323-1329 (2012).
36. McDonald, B., et al.Interaction of CD44 and hyaluronan is the dominant mechanism for neutrophil sequestration in inflamed liver sinusoids. J Exp Med. 205, (4), 915-927 (2008).
37. Egen, J. G., et al.Intravital imaging reveals limited antigen presentation and T cell effector function in mycobacterial granulomas. Immunity. 34, (5), 807-819 (2011).
38. Singer, G., Stokes, K. Y., Granger, D. N. Hepatic microcirculation in murine sepsis: role of lymphocytes. Pediatr Surg Int. 24, (1), 13-20 (2008).
39. Phillipson, M., Kubes, P. The neutrophil in vascular inflammation. Nat Med. 17, (11), 1381-1390 (2011).
40. Khandoga, A. G., et al.In vivo imaging and quantitative analysis of leukocyte directional migration and polarization in inflamed tissue. PLoS One. 4, (3), e4693 (2009).