Comparison of human Nrf2 antibodies: A tale of two proteins

Toxicology Letters

Volumn 238, Issue 2, 2015, Pages 83-89

  Kemmerer, Zachary A ^a   Ader, Nicholas R ^a   Mulroy, Sarah S ^a   Eggler, Aimee L ^a  

^a VILLANOVA UNIVERSITY   (United States)

Author keywords

Antibody; Nrf2; Western blotting

Indexed keywords

MONOCLONAL ANTIBODY; POLYACRYLAMIDE GEL; PROTEIN ANTIBODY; SMALL INTERFERING RNA; TRANSCRIPTION FACTOR NRF2; TRANSCRIPTION FACTOR NRF2 ANTIBODY; UNCLASSIFIED DRUG; NFE2L2 PROTEIN, HUMAN;

ARTICLE; CELL CULTURE; CELLULAR DISTRIBUTION; COMPARATIVE STUDY; CONTROLLED STUDY; HEPG2 CELL LINE; HUMAN; HUMAN CELL; INTRACELLULAR SIGNALING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; UBIQUITINATION; WESTERN BLOTTING; WHOLE CELL; ANTAGONISTS AND INHIBITORS; ANTIBODY SPECIFICITY; CHEMISTRY; DRUG EFFECTS; GENETIC TRANSFECTION; IMMUNOLOGY; METABOLISM; MOLECULAR WEIGHT; RNA INTERFERENCE; SIGNAL TRANSDUCTION;

ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HEP G2 CELLS; HUMANS; MOLECULAR WEIGHT; NF-E2-RELATED FACTOR 2; RNA INTERFERENCE; SIGNAL TRANSDUCTION; TRANSFECTION;

EID: 84938866907     PISSN: 03784274     EISSN: 18793169     Source Type: Journal    
DOI: 10.1016/j.toxlet.2015.07.004     Document Type: Article

References (25)

1. Apopa P.L., He X., Ma Q. Phosphorylation of Nrf2 in the transcription activation domain by casein kinase 2 (CK2) is critical for the nuclear translocation and transcription activation function of Nrf2 in IMR-32 neuroblastoma cells. J. Biochem. Mol. Toxicol. 2008, 22:63-76. 10.1002/jbt.20212.
2. Baird L., Dinkova-Kostova A. The cytoprotective role of the Keap1-Nrf2 pathway. Arch. Toxicol. 2011, 85:241-272. 10.1007/s00204-011-0674-5.
3. Baker M. Reproducibility crisis: blame it on the antibodies. Nature 2015, 521:274-276. 10.1038/521274a.
4. Boukamp P., Petrussevska R.T., Breitkreutz D., Hornung J., Markham A., Fusenig N.E. Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line. J. Cell Biol. 1988, 106:761-771. 10.1083/jcb.106.3.761.
5. Deighton R.F., Markus N.M., Al-Mubarak B., Bell K.F., Papadia S., Meakin P.J., Chowdhry S., Hayes J.D., Hardingham G.E. Nrf2 target genes can be controlled by neuronal activity in the absence of Nrf2 and astrocytes. Proc. Natl. Acad. Sci. U. S. A. 2014, 111:E1818-1820. 10.1073/pnas. 1402097111.
6. Furusawa Y., Uruno A., Yagishita Y., Higashi C., Yamamoto M. Nrf2 induces fibroblast growth factor 21 in diabetic mice. Genes Cells 2014, 19:864-878. 10.1111/gtc.12186.
7. Gao B., Doan A., Hybertson B.M. The clinical potential of influencing Nrf2 signaling in degenerative and immunological disorders. Clin. Pharmacol. 2014, 6:19-34. 10.2147/cpaa.s35078.
8. Hayes J.D., Dinkova-Kostova A.T. The Nrf2 regulatory network provides an interface between redox and intermediary metabolism. Trends Biochem. Sci. 2014, 39:199-218. 10.1016/j.tibs.2014.02.002.
9. Jennings P., Limonciel A., Felice L., Leonard M. An overview of transcriptional regulation in response to toxicological insult. Arch. Toxicol. 2013, 87:49-72. 10.1007/s00204-012-0919-y.
10. Knowles B.B., Howe C.C., Aden D.P. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 1980, 209:497-499. 10.2307/1685123.
11. Lau A., Tian W., Whitman S.A., Zhang D.D. The predicted molecular weight of Nrf2: it is what it is not. Antioxid. Redox Signal. 2013, 18:91-93. 10.1089/ars.2012.4754.
12. Lee J.M., Moehlenkamp J.D., Hanson J.M., Johnson J.A. Nrf2-dependent activation of the antioxidant responsive element by tert-butylhydroquinone is independent of oxidative stress in IMR-32 human neuroblastoma cells. Biochem. Biophys. Res. Commun. 2001, 280:286-292.
13. Ludtmann M.H., Angelova P.R., Zhang Y., Abramov A.Y., Dinkova-Kostova A.T. Nrf2 affects the efficiency of mitochondrial fatty acid oxidation. Biochem. J. 2014, 457:415-424. 10.1042/bj20130863.
14. Ma Q. Role of Nrf2 in oxidative stress and toxicity. Annu. Rev. Pharmacol. Toxicol. 2013, 53:401-426. 10.1146/annurev-pharmtox-011112-140320.
15. McMahon M., Itoh K., Yamamoto M., Hayes J.D. Keap1-dependent proteasomal degradation of transcription factor Nrf2 contributes to the negative regulation of antioxidant response element-driven gene expression. J. Biol. Chem. 2003, 278:21592-21600. 10.1074/jbc.M300931200.
16. Moi P., Chan K., Asunis I., Cao A., Kan Y.W. Isolation of NF-E2-related factor 2 (Nrf2), a NF-E2-like basic leucine zipper transcriptional activator that binds to the tandem NF-E2/AP1 repeat of the beta-globin locus control region. Proc. Natl. Acad. Sci. 1994, 91:9926-9930.
17. Namani A., Li Y., Wang X.J., Tang X. Modulation of NRF2 signaling pathway by nuclear receptors: implications for cancer. Biochim. Biophys. Acta 2014, 1843:1875-1885. 10.1016/j.bbamcr.2014.05.003.
18. Nguyen T., Sherratt P.J., Nioi P., Yang C.S., Pickett C.B. Nrf2 controls constitutive and inducible expression of ARE-driven genes through a dynamic pathway involving nucleocytoplasmic shuttling by Keap1. J. Biol. Chem. 2005, 280:32485-32492. 10.1074/jbc.M503074200.
19. Niture S.K., Jain A.K., Jaiswal A.K. Antioxidant-induced modification of INrf2 cysteine 151 and PKC-delta-mediated phosphorylation of Nrf2 serine 40 are both required for stabilization and nuclear translocation of Nrf2 and increased drug resistance. J. Cell. Sci. 2009, 122:4452-4464.
20. Pfeifer A.M., Cole K.E., Smoot D.T., Weston A., Groopman J.D., Shields P.G., Vignaud J.M., Juillerat M., Lipsky M.M., Trump B.F. Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens. Proc. Natl. Acad. Sci. 1993, 90:5123-5127.
21. Pi J., Bai Y., Reece J.M., Williams J., Liu D., Freeman M.L., Fahl W.E., Shugar D., Liu J., Qu W., Collins S., Waalkes M.P. Molecular mechanism of human Nrf2 activation and degradation: Role of sequential phosphorylation by protein kinase CK2. Free Radic. Biol. Med. 2007, 42:1797-1806.
22. Venugopal R., Jaiswal A.K. Nrf2 and Nrf1 in association with Jun proteins regulate antioxidant response element-mediated expression and coordinated induction of genes encoding detoxifying enzymes. Oncogene 1998, 17:3145-3156.
23. Vomhof-Dekrey E.E., Picklo M.J. The Nrf2-antioxidant response element pathway: a target for regulating energy metabolism. J. Nutr. Biochem. 2012, 23:1201-1206. 10.1016/j.jnutbio.2012.03.005.
24. Yueh M.F., Tukey R.H. Nrf2-Keap1 signaling pathway regulates human UGT1A1 expression in vitro and in transgenic UGT1 mice. J. Biol. Chem. 2007, 282:8749-8758. 10.1074/jbc.M610790200.
25. Zucker S.N., Fink E.E., Bagati A., Mannava S., Bianchi-Smiraglia A., Bogner P.N., Wawrzyniak J.A., Foley C., Leonova K.I., Grimm M.J., Moparthy K., Ionov Y., Wang J., Liu S., Sexton S., Kandel E.S., Bakin A.V., Zhang Y., Kaminski N., Segal B.H., Nikiforov M.A. Nrf2 amplifies oxidative stress via induction of Klf9. Mol. Cell 2014, 53:916-928. 10.1016/j.molcel.2014.01.033.