Purification of stabilized GPCRs for structural and biophysical analyses

Methods in Molecular Biology

Volumn 1335, Issue , 2015, Pages

  Errey, James C ^a   Doré, Andrew S ^a   Zhukov, Andrei ^a   Marshall, Fiona H ^a   Cooke, Robert M ^a  

^a NONE

Author keywords

Crystallization; G protein; GPCR; LCP; Membrane protein; Purification; SPR; StaR; Thermostabilization

Indexed keywords

DETERGENT; FLUOROCHROME; G PROTEIN COUPLED RECEPTOR; MALEIMIDE DERIVATIVE; MEMBRANE PROTEIN; PHOSPHOTRANSFERASE; STAR PROTEIN; THIOL; UNCLASSIFIED DRUG; 8-(4-((2-AMINOETHYL)AMINOCARBONYLMETHYLOXY)PHENYL)-1,3-DIPROPYLXANTHINE; ADENOSINE A2A RECEPTOR; IMMOBILIZED PROTEIN; LIGAND; MALEIMIDE; METABOTROPIC RECEPTOR; XANTHINE DERIVATIVE;

ARTICLE; BIOPHYSICS; CONTROLLED STUDY; CRYSTALLIZATION; GENE MUTATION; MEMBRANE BIOLOGY; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN PURIFICATION; PROTEIN STABILITY; PROTEIN STRUCTURE; SCREENING TEST; SURFACE PLASMON RESONANCE; THERMOSTABILITY; CHEMISTRY; FRACTIONATION; ISOLATION AND PURIFICATION; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROCEDURES;

BIOPHYSICAL PROCESSES; CHEMICAL FRACTIONATION; CRYSTALLIZATION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; IMMOBILIZED PROTEINS; LIGANDS; MALEIMIDES; PROTEIN STABILITY; RECEPTOR, ADENOSINE A2A; RECEPTORS, METABOTROPIC GLUTAMATE; XANTHINES;

EID: 84938866055     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2914-6_1     Document Type: Article

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