Quantifying domain-ligand affinities and specificities by high-throughput holdup assay

Nature Methods

Volumn 12, Issue 8, 2015, Pages 787-793

  Vincentelli, Renaud ^a   Luck, Katja ^b,c,f   Poirson, Juline ^b   Polanowska, Jolanta ^c,f   Abdat, Julie ^a   Blémont, Marilyne ^a   Turchetto, Jeremy ^a   Iv, François ^a   Ricquier, Kevin ^d   Straub, Marie Laure ^b   Forster, Anne ^b   Cassonnet, Patricia ^e   Borg, Jean Paul ^c   Jacob, Yves ^e   Masson, Murielle ^b   Nominé, Yves ^b   Reboul, Jérôme ^c,f   Wolff, Nicolas ^d   Charbonnier, Sebastian ^b   Travé, Gilles ^b  

^a CNRS   (France)

^b UNIVERSITÉ DE STRASBOURG   (France)

^c AIX MARSEILLE UNIVERSITY   (France)

^d INSTITUT PASTEUR   (France)

^e Sorbonne Paris Cité   (France)

^f HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AVIDIN; CELL PROTEIN; LIGAND; ONCOPROTEIN; PDZ PROTEIN; RESIN; DNA BINDING PROTEIN; E6 PROTEIN, HUMAN PAPILLOMAVIRUS TYPE 16; E6 PROTEIN, HUMAN PAPILLOMAVIRUS TYPE 18; PROTEIN; PROTEOME; REPRESSOR PROTEIN;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; CAPILLARY ELECTROPHORESIS; CELL JUNCTION; CELL POLARITY; CONTROLLED STUDY; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; HUMAN PAPILLOMAVIRUS TYPE 16; HUMAN PAPILLOMAVIRUS TYPE 18; HUMAN PAPILLOMAVIRUS TYPE 6; MAMMAL CELL; MOLECULAR RECOGNITION; NONHUMAN; PDZ DOMAIN; PHYSICAL CHEMISTRY; PRIORITY JOURNAL; SURFACE PLASMON RESONANCE; CHEMISTRY; CHROMATOGRAPHY; KINETICS; PROCEDURES; PROTEIN ANALYSIS; PROTEIN CONFORMATION; PROTEIN MOTIF; SYSTEMS BIOLOGY;

HUMAN PAPILLOMAVIRUS;

AMINO ACID MOTIFS; CHROMATOGRAPHY; DNA-BINDING PROTEINS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; KINETICS; LIGANDS; ONCOGENE PROTEINS, VIRAL; PDZ DOMAINS; PROTEIN CONFORMATION; PROTEIN INTERACTION MAPPING; PROTEINS; PROTEOME; REPRESSOR PROTEINS; SYSTEMS BIOLOGY;

EID: 84938423622     PISSN: 15487091     EISSN: 15487105     Source Type: Journal    
DOI: 10.1038/nmeth.3438     Document Type: Article

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