Discovery and characterization of thermophilic limonene-1,2-epoxide hydrolases from hot spring metagenomic libraries

FEBS Journal

Volumn 282, Issue 15, 2015, Pages 2879-2894

  Ferrandi, Erica Elisa ^a,b   Sayer, Christopher ^c   Isupov, Michail N ^c   Annovazzi, Celeste ^a   Marchesi, Carlotta ^a   Iacobone, Gianluca ^a   Peng, Xu ^b   Bonch Osmolovskaya, Elizaveta ^d   Wohlgemuth, Roland ^e   Littlechild, Jennifer A ^c   Monti, Daniela ^a  

^a CNR   (Italy)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

^c UNIVERSITY OF EXETER   (United Kingdom)

^d WINOGRADSKY INSTITUTE OF MICROBIOLOGY   (Russian Federation)

^e Sigma Aldrich   (Switzerland)

Author keywords

industrial biocatalysis; limonene 1,2 epoxide hydrolases; metagenomics; protein structure; stereoselectivity

Indexed keywords

EPOXIDE HYDROLASE; LIMONEN 1,2 EPOXIDE HYDROLASE; MACROGOL; UNCLASSIFIED DRUG; VALPROMIDE; BACTERIAL PROTEIN; LIMONENE-1,2-EPOXIDE HYDROLASE; WATER;

AMINO ACID SEQUENCE; ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; CHINA; CIS TRANS ISOMERISM; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME MECHANISM; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME SUBSTRATE; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; METAGENOMICS; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RHODOCOCCUS ERYTHROPOLIS; RUSSIAN FEDERATION; SEQUENCE ANALYSIS; STEREOCHEMISTRY; THERMAL SPRING; THERMOPHILIC BACTERIUM; CHEMISTRY; GENETICS; HEAT; METABOLISM; METAGENOME; MOLECULAR CLONING; MOLECULAR GENETICS; SEQUENCE HOMOLOGY;

RHODOCOCCUS; RHODOCOCCUS ERYTHROPOLIS;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CATALYTIC DOMAIN; CLONING, MOLECULAR; EPOXIDE HYDROLASES; ESCHERICHIA COLI; HOT TEMPERATURE; METAGENOME; MOLECULAR SEQUENCE DATA; SEQUENCE HOMOLOGY, AMINO ACID; WATER;

EID: 84938199123     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/febs.13328     Document Type: Article

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