Distribution of mitochondrial DNA nucleoids inside the linear tubules vs. bulk parts of mitochondrial network as visualized by 4Pi microscopy

Journal of Bioenergetics and Biomembranes

Volumn 47, Issue 3, 2015, Pages 255-263

  Dlasková, Andrea ^a   Engstová, Hana ^a   Plecitá – Hlavatá, Lydie ^a   Lessard, Mark ^b   Alán, Lukáš ^a   Reguera, David Pajuelo ^a   Jabůrek, Martin ^a   Ježek, Petr ^a  

^a INSTITUTE OF PHYSIOLOGY   (Czech Republic)

^b JACKSON LABORATORY   (United States)

Author keywords

4Pi microscopy; Mitochondrial DNA; Mitochondrial network; Nucleoids

Indexed keywords

DNA BINDING PROTEIN; GREEN FLUORESCENT PROTEIN; MITOCHONDRIAL DNA; MITOCHONDRIAL PROTEIN; TFAM PROTEIN, HUMAN; TRANSCRIPTION FACTOR;

CELL CULTURE TECHNIQUE; CHEMICAL STRUCTURE; CONFOCAL MICROSCOPY; CONFORMATION; FLUORESCENCE MICROSCOPY; GENETICS; HEPG2 CELL LINE; HUMAN; IMAGE PROCESSING; IMMUNOHISTOCHEMISTRY; METABOLISM; PROCEDURES;

CELL CULTURE TECHNIQUES; DNA, MITOCHONDRIAL; DNA-BINDING PROTEINS; GREEN FLUORESCENT PROTEINS; HEP G2 CELLS; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; IMMUNOHISTOCHEMISTRY; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; MITOCHONDRIAL PROTEINS; MODELS, MOLECULAR; NUCLEIC ACID CONFORMATION; TRANSCRIPTION FACTORS;

EID: 84937765227     PISSN: 0145479X     EISSN: 15736881     Source Type: Journal    
DOI: 10.1007/s10863-015-9610-3     Document Type: Article

References (25)

1. Bahlmann K, Jakobs S, Hell SW (2001) 4Pi-confocal microscopy of live cells. Ultramicroscopy 87:155–164
2. Bogenhagen DF, Rousseau D, Burke S (2008) The layered structure of human mitochondrial DNA nucleoids. J Biol Chem 283:3665–3675
3. Brown TA, Tkachuk AN, Shtengel G, Kopek BG, Bogenhagen DF, Hess HF, Clayton DA (2011) Superresolution fluorescence imaging of mitochondrial nucleoids reveals their spatial range, limits, and membrane interaction. Mol Cell Biol 31:4994–5010
4. Cree LM, Samuels DC, Lopes S, Rajasimha HK, Wonnapinij P, Mann JR, Dahl HHM, Chinnery PF (2008) A reduction of mitochondrial DNA molecules during embryogenesis explains the rapid segregation of genotypes. Nat Genet 40:249–254
5. Dlasková A, Špaček T, Šantorová J, Plecitá-Hlavatá L, Berková Z, Saudek F, Lessard M, Bewersdorf J, Ježek P (2010) 4Pi microscopy reveals an impaired three-dimensional mitochondrial network of pancreatic islet beta-cells in an experimental model of type-2 diabetes. Biochim Biophys Acta Bioenerg 1797:1327–1341
6. Egner A, Jakobs S, Hell SW (2002) Fast 100-nm resolution three-dimensional microscope reveals structural plasticity of mitochondria in live yeast. Proc Natl Acad Sci U S A 99:3370–3375
7. Gilkerson RW, Schon EA, Hernandez E, Davidson MM (2008) Mitochondrial nucleoids maintain genetic autonomy but allow for functional complementation. J Cell Biol 181:1117–1128
8. Gomes LC, Benedetto GD, Scorrano L (2011) During autophagy mitochondria elongate, are spared from degradation and sustain cell viability. Nat Cell Biol 13:589–598
9. Gugel H, Bewersdorf J, Jakobs S, Engelhardt J, Storz R, Hell SW (2004) Cooperative 4Pi excitation and detection yields sevenfold sharper optical sections in live-cell microscopy. Biophys J 87:4146–4152
10. Hell SW, Stelzer EHK (1992a) Properties of a 4Pi-confocal fluorescence microscope. J Opt Soc Am A 9:2159–2166
11. Hell SW, Stelzer EHK (1992b) Fundamental improvement of resolution with a 4Pi-confocal fluorescence microscope using two-photon excitation. Opt Commun 93:277–282
12. Holt IJ, He J, Mao CC, Boyd-Kirkup JD, Martinsson P, Sembongi H, Reyes A, Spelbrink JN (2007) Mammalian mitochondrial nucleoids: organizing an independently minded genome. Mitochondrion 7:311–321
13. Ježek P, Plecitá-Hlavatá L (2009) Mitochondrial reticulum network dynamics in relation to oxidative stress, redox regulation, and hypoxia. Int J Biochem Cell Biol 41:1790–1804
14. Kopek BG, Shtengel G, Xu CS, Clayton DA, Hess HF (2012) Correlative 3D superresolution fluorescence and electron microscopy reveal the relationship of mitochondrial nucleoids to membranes. Proc Natl Acad Sci U S A 109:6136–6141
15. Kukat C, Wurm CA, Spahr H, Falkenberg M, Larsson NG, Jacobs S (2011) Super-resolution microscopy reveals that mammalian mitochondrial nucleoids have a uniform size and frequently contain a single copy of mtDNA. Proc Natl Acad Sci U S A 108:13534–13539
16. Mlodzianoski MJ, Schreiner JM, Callahan SP, Smolková K, Dlasková A, Šantorová J, Ježek P, Bewersdorf J (2011) Sample drift correction in 3D fluorescence photoactivation localization microscopy. Opt Express 19:15009–15019
17. Plecitá-Hlavatá L, Lessard M, Šantorová J, Bewersdorf J, Ježek P (2008) Mitochondrial oxidative phosphorylation and energetic status are reflected by morphology of mitochondrial network in INS-1E and HEP-G2 cells viewed by 4Pi microscopy. Biochim Biophys Acta 1777:834–846
18. Rossignol R, Gilkerson R, Aggeler R, Yamagata K, Remington SJ, Capaldi RA (2004) Energy substrate modulates mitochondrial structure and oxidative capacity in cancer cells. Cancer Res 64:985–993
19. Spelbrink JN (2010) Functional organization of mammalian mitochondrial DNA in nucleoids: history, recent developments, and future challenges. IUBMB Life 62:19–32
20. Stewart JB, Larsson NG (2014) Keeping mtDNA in shape between generations. PLoS Genet 10:e1004670
21. Stewart JB, Treter C, Elson JL, Wredenberg A, Cansu Z, Trifunovic A, Larsson NG (2008) Strong purifying selection in transmission of mammalian mitochondrial DNA. PLoS Biol 6:e10
22. Tauber J, Dlasková A, Šantorová J, Smolková K, Alán L, Špaček T, Plecitá-Hlavatá L, Jabůrek M, Ježek P (2013) Distribution of mitochondrial nucleoids upon mitochondrial network fragmentation and network reintegration in HEPG2 cells. Int J Biochem Cell Biol 45:593–603
23. Twig G, Elorza A, Molina AJ, Mohamed H, Wikstrom JD, Walzer G, Stiles L, Haigh SE, Katz S, Las G, Alroy J, Wu M, Py BF, Yuan J, Deeney JT, Corkey BE, Shirihai OS (2008) Fission and selective fusion govern mitochondrial segregation and elimination by autophagy. EMBO J 27:433–446
24. Twig G, Liu X, Liesa M, Wikstrom JD, Molina AJ, Las G, Yaniv G, Hajnóczky G, Shirihai OS (2010) Biophysical properties of mitochondrial fusion events in pancreatic beta-cells and cardiac cells unravel potential control mechanisms of its selectivity. Am J Physiol Cell Physiol 299:C477–C487
25. Wang Y, Bogenhagen DF (2006) Human mitochondrial DNA nucleoids are linked to protein folding machinery and metabolic enzymes at the mitochondrial inner membrane. J Biol Chem 281:25791–25802