α-Defensins partially protect human neutrophils against Panton-Valentine leukocidin produced by Staphylococcus aureus

Letters in Applied Microbiology

Volumn 61, Issue 2, 2015, Pages 158-164

  Cardot Martin, E ^a,b   Casalegno, J S ^a   Badiou, C ^a,b   Dauwalder, O ^a,b   Keller, D ^c   Prevost G ^c   Rieg, S ^d   Kern, W V ^d   Cuerq, C ^b,e   Etienne, J ^a,b   Vandenesch, F ^a,b   Lina, G ^a,b   Dumitrescu, O ^a,b  

^a UNIVERSITÉ LYON 1   (France)

^b HOSPICES CIVILS DE LYON   (France)

^c UNIVERSITÉ DE STRASBOURG   (France)

^d UNIVERSITY OF FREIBURG   (Germany)

^e Centre de Biologie Sud   (France)

Author keywords

Antimicrobial peptides; Defensins; Human neutrophil peptide; Neutrophils; Panton Valentine leukocidin; Pore forming toxins; Staphylococcus aureus

Indexed keywords

BACTERIA; METABOLITES; MOLECULES; PORE SIZE; TOXIC MATERIALS;

ANTIMICROBIAL PEPTIDE; DEFENSINS; HUMAN NEUTROPHIL; HUMAN NEUTROPHILE PEPTIDE; NEUTROPHILE; PANTON-VALENTINE LEUCOCIDIN; POLYMORPHONUCLEAR NEUTROPHILS; PORE FORMATION; PORE FORMING TOXINS; STAPHYLOCOCCUS AUREUS;

PEPTIDES;

ALPHA DEFENSIN; HUMAN NEUTROPHIL PEPTIDE 1; HUMAN NEUTROPHIL PEPTIDE 2; HUMAN NEUTROPHIL PEPTIDE 3; LACTATE DEHYDROGENASE; PANTON VALENTINE LEUKOCIDIN; POLYPEPTIDE ANTIBIOTIC AGENT; PROTEIN LUKF; PROTEIN LUKS; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; BACTERIAL TOXIN; EXOTOXIN; LEUKOCIDIN; LEUKOCIDIN S-COMPONENT PROTEIN, STAPHYLOCOCCUS; LUKF PROTEIN, STAPHYLOCOCCUS AUREUS; PANTON-VALENTINE LEUKOCIDIN;

BACTERIUM; BLOOD; CALCIUM; IMMUNE SYSTEM; MICROBIAL ACTIVITY; NEUTRALIZATION; PEPTIDE; TOXIN;

ARTICLE; CALCIUM TRANSPORT; CELL PROTECTION; CHANNEL GATING; CONFORMATIONAL TRANSITION; CYTOTOXICITY; HUMAN; HUMAN CELL; NEUTROPHIL; NONHUMAN; OLIGOMERIZATION; PROTEIN BINDING; PROTEIN PROTEIN INTERACTION; STAPHYLOCOCCUS AUREUS; ENZYMOLOGY; IMMUNOLOGY; METABOLISM; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; PATHOGENICITY; STAPHYLOCOCCUS INFECTION;

BACTERIA (MICROORGANISMS); METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS AUREUS;

ALPHA-DEFENSINS; BACTERIAL PROTEINS; BACTERIAL TOXINS; EXOTOXINS; HUMANS; LEUKOCIDINS; METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS; NEUTROPHILS; STAPHYLOCOCCAL INFECTIONS;

EID: 84937023136     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/lam.12438     Document Type: Article

References (24)

1. Ashitani, J., Mukae, H., Arimura, Y., Sano, A., Tokojima, M. and Nakazato, M. (2004) High concentrations of alpha-defensins in plasma and bronchoalveolar lavage fluid of patients with acute respiratory distress syndrome. Life Sci 75, 1123-1134.
2. Baba Moussa, L., Werner, S., Colin, D.A., Mourey, L., Pedelacq, J.D., Samama, J.P., Sanni, A., Monteil, H. et al. (1999) Discoupling the Ca(2+)-activation from the pore-forming function of the bi-component Panton-Valentine leucocidin in human PMNs. FEBS Lett 461, 280-286.
3. Badiou, C., Dumitrescu, O., George, N., Forbes, A.R., Drougka, E., Chan, K.S., Ramdani-Bouguessa, N., Meugnier, H. et al. (2010) Rapid detection of Staphylococcus aureus Panton-Valentine leukocidin in clinical specimens by enzyme-linked immunosorbent assay and immunochromatographic tests. J Clin Microbiol 48, 1384-1390.
4. Brogden, K.A. (2005) Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria? Nat Rev Microbiol 3, 238-250.
5. Combet, C., Blanchet, C., Geourjon, C. and Deleage, G. (2000) NPS@: network protein sequence analysis. Trends Biochem Sci 25, 147-150.
6. Diep, B.A., Chan, L., Tattevin, P., Kajikawa, O., Martin, T.R., Basuino, L., Mai, T.T., Marbach, H. et al. (2010) Polymorphonuclear leukocytes mediate Staphylococcus aureus Panton-Valentine leukocidin-induced lung inflammation and injury. Proc Natl Acad Sci USA 107, 5587-5592.
7. Fujii, G., Selsted, M.E. and Eisenberg, D. (1993) Defensins promote fusion and lysis of negatively charged membranes. Protein Sci 2, 1301-1312.
8. Gauduchon, V., Werner, S., Prevost, G., Monteil, H. and Colin, D.A. (2001) Flow cytometric determination of Panton-Valentine leucocidin S component binding. Infect Immun 69, 2390-2395.
9. Genestier, A.L., Michallet, M.C., Prevost, G., Bellot, G., Chalabreysse, L., Peyrol, S., Thivolet, F., Etienne, J. et al. (2005) Staphylococcus aureus Panton-Valentine leukocidin directly targets mitochondria and induces Bax-independent apoptosis of human neutrophils. J Clin Invest 115, 3117-3127.
10. Giacometti, A., Cirioni, O., Del Prete, M.S., Paggi, A.M., D'Errico, M.M. and Scalise, G. (2000) Combination studies between polycationic peptides and clinically used antibiotics against Gram-positive and Gram-negative bacteria. Peptides 21, 1155-1160.
11. Giesemann, T., Guttenberg, G. and Aktories, K. (2008) Human alpha-defensins inhibit Clostridium difficile toxin B. Gastroenterology 134, 2049-2058.
12. Gillet, Y., Issartel, B., Vanhems, P., Fournet, J.C., Lina, G., Bes, M., Vandenesch, F., Piemont, Y. et al. (2002) Association between Staphylococcus aureus strains carrying gene for Panton-Valentine leukocidin and highly lethal necrotising pneumonia in young immunocompetent patients. Lancet 359, 753-759.
13. Gillet, Y., Dumitrescu, O., Tristan, A., Dauwalder, O., Javouhey, E., Floret, D., Vandenesch, F., Etienne, J. et al. (2011) Pragmatic management of Panton-Valentine leukocidin-associated staphylococcal diseases. Int J Antimicrob Agents 38, 457-464.
14. Kim, C., Gajendran, N., Mittrucker, H.W., Weiwad, M., Song, Y.H., Hurwitz, R., Wilmanns, M., Fischer, G. et al. (2005) Human alpha-defensins neutralize anthrax lethal toxin and protect against its fatal consequences. Proc Natl Acad Sci USA 102, 4830-4835.
15. Konig, B., Prevost, G., Piemont, Y. and Konig, W. (1995) Effects of Staphylococcus aureus leukocidins on inflammatory mediator release from human granulocytes. J Infect Dis 171, 607-613.
16. Lehrer, R.I., Jung, G., Ruchala, P., Wang, W., Micewicz, E.D., Waring, A.J., Gillespie, E.J., Bradley, K.A. et al. (2009) Human alpha-defensins inhibit hemolysis mediated by cholesterol-dependent cytolysins. Infect Immun 77, 4028-4040.
17. Lillard, J.W. Jr, Boyaka, P.N., Chertov, O., Oppenheim, J.J. and McGhee, J.R. (1999) Mechanisms for induction of acquired host immunity by neutrophil peptide defensins. Proc Natl Acad Sci USA 96, 651-656.
18. Meyer, F., Girardot, R., Piemont, Y., Prevost, G. and Colin, D.A. (2009) Analysis of the specificity of Panton-Valentine leucocidin and gamma-hemolysin F component binding. Infect Immun 77, 266-273.
19. Moran, G.J., Krishnadasan, A., Gorwitz, R.J., Fosheim, G.E., McDougal, L.K., Carey, R.B. and Talan, D.A. (2006) Methicillin-resistant S. aureus infections among patients in the emergency department. N Engl J Med 355, 666-674.
20. Muller, C.A., Markovic-Lipkovski, J., Klatt, T., Gamper, J., Schwarz, G., Beck, H., Deeg, M., Kalbacher, H. et al. (2002) Human alpha-defensins HNPs-1, -2, and -3 in renal cell carcinoma: influences on tumor cell proliferation. Am J Pathol 160, 1311-1324.
21. Rieg, S., Meier, B., Fahnrich, E., Huth, A., Wagner, D., Kern, W.V. and Kalbacher, H. (2010) Differential activity of innate defense antimicrobial peptides against Nocardia species. BMC Microbiol 10, 61.
22. Spaan, A.N., Henry, T., van Rooijen, W.J., Perret, M., Badiou, C., Aerts, P.C., Kemmink, J., de Haas, C.J. et al. (2013) The staphylococcal toxin Panton-Valentine Leukocidin targets human C5a receptors. Cell Host Microbe 13, 584-594.
23. Vandenesch, F., Naimi, T., Enright, M.C., Lina, G., Nimmo, G.R., Heffernan, H., Liassine, N., Bes, M. et al. (2003) Community-acquired methicillin-resistant Staphylococcus aureus carrying Panton-Valentine leukocidin genes: worldwide emergence. Emerg Infect Dis 9, 978-984.
24. Yamashita, K., Kawai, Y., Tanaka, Y., Hirano, N., Kaneko, J., Tomita, N., Ohta, M., Kamio, Y. et al. (2011) Crystal structure of the octameric pore of staphylococcal gamma-hemolysin reveals the beta-barrel pore formation mechanism by two components. Proc Natl Acad Sci USA 108, 17314-17319.