Low glucose depletes glycan precursors, reduces site occupancy and galactosylation of a monoclonal antibody in CHO cell culture

Biotechnology Journal

Volumn 10, Issue 7, 2015, Pages 1051-1066

  Villacrés, Carina ^a   Tayi, Venkata S ^a   Lattová, Erika ^a,b   Perreault, Hélène ^a   Butler, Michael ^a  

^a UNIVERSITY OF MANITOBA   (Canada)

^b MASARYK UNIVERSITY   (Czech Republic)

Author keywords

Adenylate energy charge; Chinese hamster ovary (CHO) cells; Glucose depletion; N glycosylation; Nucleotide sugars

Indexed keywords

ANTIBODIES; BATCH CELL CULTURE; CELL CULTURE; CELLS; CYTOLOGY; ESTERIFICATION; GLYCOSYLATION; MASS SPECTROMETRY; MONOCLONAL ANTIBODIES; NUCLEOTIDES; PRODUCTIVITY; SUGARS;

ADENYLATE ENERGY CHARGES; CHINESE HAMSTER OVARY CELLS; GLUCOSE DEPLETION; N-GLYCOSYLATION; NUCLEOTIDE SUGAR;

GLUCOSE;

GALACTOSE; GLUCOSE; GLYCAN; LACTIC ACID; MONOCLONAL ANTIBODY; GLYCOPROTEIN; RECOMBINANT PROTEIN;

ANTIBODY PRODUCTION; ARTICLE; CELL DENSITY; CELL METABOLISM; CHO CELL LINE; CONTROLLED STUDY; FED BATCH CULTURE; GLUCOSE INTAKE; GLUCOSE TRANSPORT; GLYCOSYLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; HYDROPHILIC INTERACTION CHROMATOGRAPHY; MASS SPECTROMETRY; PRIORITY JOURNAL; PRODUCTIVITY; PROTEIN DEPLETION; PROTEIN PURIFICATION; ANIMAL; BIOSYNTHESIS; CRICETULUS; GENETICS; HAMSTER; METABOLISM;

CRICETULUS GRISEUS; LAMA (MAMMAL);

ANIMALS; ANTIBODIES, MONOCLONAL; CHO CELLS; CRICETINAE; CRICETULUS; GLUCOSE; GLYCOPROTEINS; GLYCOSYLATION; HUMANS; RECOMBINANT PROTEINS;

EID: 84935713928     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201400662     Document Type: Article

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