Codon optimization significantly improves the expression level of α-Amylase gene from Bacillus licheniformis in Pichia pastoris

BioMed Research International

Volumn 2015, Issue , 2015, Pages

  Wang, Jian Rong ^a,b   Li, Yang Yuan ^a,b   Liu, Dan Ni ^a,b   Liu, Jing Shan ^a,b   Li, Peng ^a,b   Chen, Li Zhi ^a,b   Xu, Shu De ^a,b  

^a Guangdong VTR BIO TECH Co Ltd   (China)

^b Guangdong Feed Additive Research and Biotechnology Center   (China)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; AMYLASE; METHANOL; NUCLEOTIDE; CODON; RECOMBINANT PROTEIN;

ARTICLE; BACILLUS LICHENIFORMIS; BACTERIAL GENETICS; CELL DENSITY; CODON; CODON USAGE; CONTROLLED STUDY; DNA BASE COMPOSITION; DNA SEQUENCE; ENZYME ACTIVITY; FIBROBACTER SUCCINOGENES; GENE EXPRESSION; HETEROLOGOUS EXPRESSION; KOMAGATAELLA PASTORIS; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; BACILLUS; CHEMISTRY; ENZYMOLOGY; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PICHIA; PROTEIN ENGINEERING; SEQUENCE ALIGNMENT;

BACILLUS LICHENIFORMIS; PICHIA PASTORIS;

ALPHA-AMYLASES; BACILLUS; BASE SEQUENCE; CODON; MOLECULAR SEQUENCE DATA; PICHIA; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT;

EID: 84934971861     PISSN: 23146133     EISSN: 23146141     Source Type: Journal    
DOI: 10.1155/2015/248680     Document Type: Article

References (23)

1. R. Gupta, P. Gigras, H. Mohapatra, V. K. Goswami, and B. Chauhan, "Microbial-amylases: a biotechnological perspective, " Process Biochemistry, vol. 38, no. 11, pp. 1599-1616, 2003.
2. S. Janecek, B. Svensson, and E. A. MacGregor, "-Amylase: an enzyme specificity found in various families of glycoside hydrolases, " Cellular and Molecular Life Sciences, vol. 71, no. 7, pp. 1149-1170, 2013.
3. W. D. Crabb and C. Mitchinson, "Enzymes involved in the processing of starch to sugars, " Trends in Biotechnology, vol. 15, no. 9, pp. 349-352, 1997.
4. S. Sivaramakrishnan, D. Gangadharan, K. M. Nampoothiri, C. R. Soccol, and A. Pandey, "-amylases from microbial sources-an overview on recent developments, " Food Technology and Biotechnology, vol. 44, no. 2, pp. 173-184, 2006.
5. P. M. de Souza and P. O. Magalhães, "Application of microbial-amylase in industry-a review, " Brazilian Journal of Microbiology, vol. 41, no. 4, pp. 850-861, 2010.
6. H. Liu, J. K. Yang, and Y. J. Yan, "Cloning and expression of an-amylase gene from Bacillus subtilis, " Biotechnology, vol. 5, no. 3, pp. 104-108, 2007 (Chinese).
7. D. M. Ren, C. G. He, Z. W. Qian, X. C. Deng, and Q. Y. Yang, "Cloning and expression of two-amylase gene from Bacillus stearothermophilus in Ecoli, " Chinese Journal of Biotechnology, vol. 3, no. 5, pp. 197-202, 1987 (Chinese).
8. T. Yuuki, T. Nomura, H. Tezuka et al., "Complete nucleotide sequence of a gene coding for heat-and pH-stable-amylase of Bacillus licheniformis: comparison of the amino acid sequences of three bacterial liquefying-amylases deduced fromtheDNA sequences, " The Journal of Biochemistry, vol. 98, no. 5, pp. 1147-1156, 1985.
9. P. Joyet, M. Guerineau, and H. Heslot, "Cloning of a thermostable-amylase gene from Bacillus licheniformis and its expression in Escherichia coli and Bacillus subtilis, " FEMS Microbiology Letters, vol. 21, no. 3, pp. 353-358, 1984.
10. S. Macauley-Patrick, M. L. Fazenda, B. McNeil, and L. M. Harvey, "Heterologous protein production using the Pichia pastoris expression system, " Yeast, vol. 22, no. 4, pp. 249-270, 2005.
11. R. Ramn, P. Ferrer, and F. Valero, "Sorbitol co-feeding reduces metabolic burden caused by the overexpression of a Rhizopus oryzae lipase in Pichia pastoris, " Journal of Biotechnology, vol. 130, no. 1, pp. 39-46, 2007.
12. M. Yu, S. Wen, and T. Tan, "Enhancing production of Yarrowia lipolytica lipase Lip2 in Pichia pastoris, " Engineering in Life Sciences, vol. 10, no. 5, pp. 458-464, 2010.
13. X.-F. Wang, X.-G. Shen, Y.-C. Sun et al., "Production of Yarrowia lipolytica lipase LIP2 in Pichia pastoris using the nitrogen source-regulated FLD1 promoter, " Journal of Chemical Technology and Biotechnology, vol. 87, no. 4, pp. 553-558, 2012.
14. A.-S. Xiong, Q.-H. Yao, R.-H. Peng et al., "High level expression of a synthetic gene encoding Peniophora lycii phytase in methylotrophic yeast Pichia pastoris, " Applied Microbiology and Biotechnology, vol. 72, no. 5, pp. 1039-1047, 2006.
15. D. Teng, Y. Fan, Y.-L. Yang, Z.-G. Tian, J. Luo, and J.-H. Wang, "Codon optimization of Bacillus licheniformis-1, 3-1, 4-glucanase gene and its expression in Pichia pastoris, " Applied Microbiology and Biotechnology, vol. 74, no. 5, pp. 1074-1083, 2007.
16. J.-R. Wang, Y.-Y. Li, S.-D. Xu, P. Li, J.-S. Liu, and D.-N. Liu, "High-level expression of pro-formlipase fromRhizopus oryzae in Pichia pastoris and its purification and characterization, " International Journal of Molecular Sciences, vol. 15, no. 1, pp. 203-217, 2013.
17. Y. Liu, S. Fan, X. Liu et al., "A highly active alpha amylase from Bacillus licheniformis: directed evolution, enzyme characterization and structural analysis, " Journal of M icrobiology and Biotechnology, vol. 24, no. 2, pp. 898-904, 2014.
18. H. Y. Jia, G. S. Fan, Q. J. Yan, Y. C. Liu, Y. Yan, and Z. Q. Jiang, "High-level expression of a hyperthermostable Thermotoga maritima xylanase in Pichia pastoris by codon optimization, " Journal of Molecular Catalysis B: Enzymatic, vol. 78, pp. 72-77, 2012.
19. J. Yang and L. Liu, "Codon optimization through a two-step gene synthesis leads to a high-level expression of Aspergillus niger lip2 gene in Pichia pastoris, " Journal of Molecular Catalysis B: Enzymatic, vol. 63, no. 3-4, pp. 164-169, 2010.
20. L. N. Ten, W.-T. Im, M.-K. Kim, M. S. Kang, and S.-T. Lee, "Development of a plate technique for screening of polysaccharide-degrading microorganisms by using a mixture of insoluble chromogenic substrates, " Journal of Microbiological Methods, vol. 56, no. 3, pp. 375-382, 2004.
21. D.-M. Lan, N. Yang, W.-K. Wang, Y.-F. Shen, B. Yang, and Y.-H. Wang, "A novel cold-active lipase from Candida albicans: cloning, expression and characterization of the recombinant enzyme, " International Journal of Molecular Sciences, vol. 12, no. 6, pp. 3950-3965, 2011.
22. Y. Li, B. Zhang, X. Chen, Y. Chen, andY. Cao, "Improvement of Aspergillus sulphureus endo-1, 4-xylanase expression in Pichia pastoris by codon optimization and analysis of the enzymic characterization, " Applied Biochemistry and Biotechnology, vol. 160, no. 5, pp. 1321-1331, 2010.
23. H. Huang, P. Yang, H. Luo et al., "High-level expression of a truncated 1, 3-1, 4-D-glucanase from Fibrobacter succinogenes in Pichia pastoris by optimization of codons and fermentation, " Applied Microbiology and Biotechnology, vol. 78, no. 1, pp. 95-103, 2008.