High-level expression of pro-form lipase from rhizopus oryzae in pichia pastoris and its purification and characterization

International Journal of Molecular Sciences

Volumn 15, Issue 1, 2014, Pages 203-217

  Wang, Jian Rong ^a,b   Li, Yang Yuan ^a,b   Xu, Shu De ^a,b   Li, Peng ^a,b   Liu, Jing Shan ^a,b   Liu, Dan Ni ^a,b  

^a Guangdong VTR Bio Tech Co   (China)

^b Guangdong Feed Additive Research and Development Center   (China)

Author keywords

Expression; Lipase; Pichia pastoris; Rhizopus oryzae

Indexed keywords

CALCIUM ION; DETERGENT; DISSOLVED OXYGEN; MERCURY; METAL ION; ORGANIC SOLVENT; SILVER; TRIACYLGLYCEROL LIPASE; FUNGAL PROTEIN; ION; METAL; RECOMBINANT PROTEIN;

ARTICLE; BIOMASS; CELL DENSITY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; FERMENTATION; GENE SEQUENCE; ION EXCHANGE CHROMATOGRAPHY; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PICHIA PASTORIS; POLYMERASE CHAIN REACTION; RHIZOPUS ORYZAE; TEMPERATURE; BATCH CELL CULTURE; BIOSYNTHESIS; CHEMISTRY; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; PICHIA; RHIZOPUS; ULTRAFILTRATION;

PICHIA PASTORIS; RHIZOPUS ORYZAE;

BATCH CELL CULTURE TECHNIQUES; CHROMATOGRAPHY, ION EXCHANGE; CLONING, MOLECULAR; FUNGAL PROTEINS; HYDROGEN-ION CONCENTRATION; IONS; LIPASE; METALS; PICHIA; RECOMBINANT PROTEINS; RHIZOPUS; SUBSTRATE SPECIFICITY; TEMPERATURE; ULTRAFILTRATION;

EID: 84891398357     PISSN: 16616596     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms15010203     Document Type: Article

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