메뉴 건너뛰기




Volumn 488, Issue , 2008, Pages 357-365

Preparation of efficient splicing extracts from whole cells, nuclei, and cytoplasmic fractions

Author keywords

Nuclear extracts; S100 extracts; Sonication; Splicing; Whole cell extracts

Indexed keywords

EUKARYOTA;

EID: 84934444843     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60327-475-3_23     Document Type: Article
Times cited : (24)

References (20)
  • 1
    • 0034069505 scopus 로고    scopus 로고
    • Mechanisms of fidelity in pre-mRNA splicing
    • Reed, R. (2000) Mechanisms of fidelity in pre-mRNA splicing. Curr. Opin. Cell Biol. 12, 340-345.
    • (2000) Curr. Opin. Cell Biol , vol.12 , pp. 340-345
    • Reed, R.1
  • 2
    • 0035370845 scopus 로고    scopus 로고
    • Pre-mRNA splicing in the new millennium
    • Hastings, M. L., and Krainer, A. R. (2001) Pre-mRNA splicing in the new millennium. Curr. Opin. Cell Biol. 13, 302-309.
    • (2001) Curr. Opin. Cell Biol , vol.13 , pp. 302-309
    • Hastings, M.L.1    Krainer, A.R.2
  • 3
    • 0042671357 scopus 로고    scopus 로고
    • Pre-mRNA splicing: Awash in a sea of proteins
    • Jurica, M. S., and Moore, M. J. (2003) Pre-mRNA splicing: awash in a sea of proteins. Mol. Cell. 12, 5-14.
    • (2003) Mol. Cell , vol.12 , pp. 5-14
    • Jurica, M.S.1    Moore, M.J.2
  • 4
    • 0024003023 scopus 로고
    • Accurate and efficient pre-mRNA splicing in Drosophila cellfree extracts
    • Rio, D. C. (1988) Accurate and efficient pre-mRNA splicing in Drosophila cellfree extracts. Proc. Natl. Acad. Sci. U. S. A. 85, 2904-2908.
    • (1988) Proc. Natl. Acad. Sci. U. S. A , vol.85 , pp. 2904-2908
    • Rio, D.C.1
  • 6
    • 0021100690 scopus 로고
    • Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei
    • Dignam, J. D., Lebovitz, R. M., and Roeder, R. G. (1983) Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res. 11, 1475-1489.
    • (1983) Nucleic Acids Res , vol.11 , pp. 1475-1489
    • Dignam, J.D.1    Lebovitz, R.M.2    Roeder, R.G.3
  • 7
    • 1342325392 scopus 로고    scopus 로고
    • A simple whole-cell lysate system for in vitro splicing reveals a stepwise-assembly of the exon-exon junction complex
    • Kataoka, N., and Dreyfuss, G. (2004) A simple whole-cell lysate system for in vitro splicing reveals a stepwise-assembly of the exon-exon junction complex. J. Biol. Chem. 279, 7009-7013.
    • (2004) J. Biol. Chem , vol.279 , pp. 7009-7013
    • Kataoka, N.1    Dreyfuss, G.2
  • 8
    • 0025367075 scopus 로고
    • The essential pre-mRNA splicing factor SF2 influences 5′ splice site selection by activating proximal sites
    • Krainer, A. R., Conway, G. C., and Kozak, D. (1990) The essential pre-mRNA splicing factor SF2 influences 5′ splice site selection by activating proximal sites. Cell 62, 35-42.
    • (1990) Cell , vol.62 , pp. 35-42
    • Krainer, A.R.1    Conway, G.C.2    Kozak, D.3
  • 9
    • 0029372979 scopus 로고
    • The superfamily of arginine/serine-rich splicing factors
    • Fu, X. D. (1995) The superfamily of arginine/serine-rich splicing factors. RNA 1, 663-680.
    • (1995) RNA , vol.1 , pp. 663-680
    • Fu, X.D.1
  • 10
    • 0033835333 scopus 로고    scopus 로고
    • Sorting out the complexity of SR protein functions
    • Graveley, B. R. (2000) Sorting out the complexity of SR protein functions. RNA 6, 1197-1211.
    • (2000) RNA , vol.6 , pp. 1197-1211
    • Graveley, B.R.1
  • 11
    • 0026543785 scopus 로고
    • Regulation of alternative pre-mRNA splicing by hnRNP A1 and splicing factor SF2
    • Mayeda, A., and Krainer, A. R. (1992) Regulation of alternative pre-mRNA splicing by hnRNP A1 and splicing factor SF2. Cell 68, 365-375.
    • (1992) Cell , vol.68 , pp. 365-375
    • Mayeda, A.1    Krainer, A.R.2
  • 12
    • 0033013977 scopus 로고    scopus 로고
    • Substrate specificities of SR proteins in constitutive splicing are determined by their RNA recognition motifs and composite pre-mRNA exonic elements
    • Mayeda, A., Screaton, G. R., Chandler, S. D., Fu, X. D., and Krainer, A. R. (1999) Substrate specificities of SR proteins in constitutive splicing are determined by their RNA recognition motifs and composite pre-mRNA exonic elements. Mol. Cell. Biol. 19, 1853-1863.
    • (1999) Mol. Cell. Biol , vol.19 , pp. 1853-1863
    • Mayeda, A.1    Screaton, G.R.2    Chandler, S.D.3    Fu, X.D.4    Krainer, A.R.5
  • 13
    • 0029863992 scopus 로고    scopus 로고
    • A novel spliceosome containing U11, U12, and U5 snRNPs excises a minor class (AT-AC) intron in vitro
    • Tarn, W. Y., and Steitz, J. A. (1996) A novel spliceosome containing U11, U12, and U5 snRNPs excises a minor class (AT-AC) intron in vitro. Cell 84, 801-811.
    • (1996) Cell , vol.84 , pp. 801-811
    • Tarn, W.Y.1    Steitz, J.A.2
  • 14
    • 0030575916 scopus 로고    scopus 로고
    • U1-mediated exon definition interactions between AT-AC and GT-AG introns
    • Wu, Q., and Krainer, A. R. (1996) U1-mediated exon definition interactions between AT-AC and GT-AG introns. Science 274, 1005-1008.
    • (1996) Science , vol.274 , pp. 1005-1008
    • Wu, Q.1    Krainer, A.R.2
  • 15
    • 0032567036 scopus 로고    scopus 로고
    • A novel function for SMN, the spinal muscular atrophy disease gene product, in pre-mRNA splicing
    • Pellizzoni, L., Kataoka, N., Charroux, B., and Dreyfuss, G. (1998) A novel function for SMN, the spinal muscular atrophy disease gene product, in pre-mRNA splicing. Cell 95, 615-624.
    • (1998) Cell , vol.95 , pp. 615-624
    • Pellizzoni, L.1    Kataoka, N.2    Charroux, B.3    Dreyfuss, G.4
  • 16
    • 0033634824 scopus 로고    scopus 로고
    • Pre-mRNA splicing imprints mRNA in the nucleus with a novel RNA-binding protein that persists in the cytoplasm
    • Kataoka, N., Yong, J., Kim, V. N., et al. (2000) Pre-mRNA splicing imprints mRNA in the nucleus with a novel RNA-binding protein that persists in the cytoplasm. Mol. Cell. 6, 673-682.
    • (2000) Mol. Cell , vol.6 , pp. 673-682
    • Kataoka, N.1    Yong, J.2    Kim, V.N.3
  • 17
    • 0035890258 scopus 로고    scopus 로고
    • Magoh, a human homolog of Drosophila mago nashi protein, is a component of splicing-dependent exon-exon junction complex
    • Kataoka, N., Diem, M. D., Kim, V. N., Yong, J., and Dreyfuss, G. (2001) Magoh, a human homolog of Drosophila mago nashi protein, is a component of splicing-dependent exon-exon junction complex. EMBO J. 20, 6424-6433.
    • (2001) EMBO J , vol.20 , pp. 6424-6433
    • Kataoka, N.1    Diem, M.D.2    Kim, V.N.3    Yong, J.4    Dreyfuss, G.5
  • 18
    • 0035823150 scopus 로고    scopus 로고
    • Role of the nonsense-mediated decay factor hUpf3 in the splicing-dependent exon-exon junction complex
    • Kim, V. N., Kataoka, N., and Dreyfuss, G. (2001) Role of the nonsense-mediated decay factor hUpf3 in the splicing-dependent exon-exon junction complex. Science 293, 1832-1836.
    • (2001) Science , vol.293 , pp. 1832-1836
    • Kim, V.N.1    Kataoka, N.2    Dreyfuss, G.3
  • 19
    • 1542361776 scopus 로고    scopus 로고
    • eIF4A3 is a novel component of the exon junction complex
    • Chan, C. C., Dostie, J., Diem, M. D., et al. (2004) eIF4A3 is a novel component of the exon junction complex. RNA 10, 200-209.
    • (2004) RNA , vol.10 , pp. 200-209
    • Chan, C.C.1    Dostie, J.2    Diem, M.D.3
  • 20
    • 0023449311 scopus 로고
    • In vitro splicing of a chicken delta-crystallin pre-mRNA in a mammalian nuclear extract
    • Sakamoto, H., Ohno, M., Yasuda, K., Mizumoto, K., and Shimura, Y. (1987) In vitro splicing of a chicken delta-crystallin pre-mRNA in a mammalian nuclear extract. J. Biochem. (Tokyo) 102, 1289-1301.
    • (1987) J. Biochem. (Tokyo) , vol.102 , pp. 1289-1301
    • Sakamoto, H.1    Ohno, M.2    Yasuda, K.3    Mizumoto, K.4    Shimura, Y.5


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.