Nanometer-resolution fluorescence electron microscopy (Nano-EM) in cultured cells

Methods in Molecular Biology

Volumn 1117, Issue , 2014, Pages 503-526

  Watanabe, Shigeki ^a,b   Lehmann, Martin ^c   Hujber, Edward ^a,b   Fetter, Richard D ^d   Richards, Jackson ^a,b   Söhl Kielczynski, Berit ^e   Felies, Annegret ^e   Rosenmund, Christian ^e   Schmoranzer, Jan ^c   Jorgensen, Erik M ^a,b  

^a UNIVERSITY OF UTAH   (United States)

^b HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^c LEIBNIZ INSTITUT FÜR MOLEKULARE PHARMAKOLOGIE   (Germany)

^d HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^e CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

Correlative light and electron microscopy; Direct stochastic optical reconstruction microscopy (dSTORM); Fluorescence electron microscopy; Fluorescence nanoscopy; Ground state depletion and single molecule return (GSDIM); Nano fEM; Photoactivated localization microscopy (PALM); Protein localization; Stochastic optical reconstruction microscopy (STORM); Super resolution fluorescence microscopy

Indexed keywords

URANYL ACETATE;

ARTICLE; ELECTRON MICROSCOPE; ELECTRON MICROSCOPY; FLUORESCENCE; FREEZE DRYING; NANO RESOLUTION FLUORESCENCE ELECTRON MICROSCOPY; PHOTOACTIVATION; PRIORITY JOURNAL; TISSUE CULTURE CELL;

ANIMALS; CAENORHABDITIS ELEGANS; CELLS, CULTURED; HISTOCYTOLOGICAL PREPARATION TECHNIQUES; IMAGE PROCESSING, COMPUTER-ASSISTED; MICE; MICROSCOPY, ELECTRON; MICROSCOPY, FLUORESCENCE;

EID: 84934444073     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-776-1_22     Document Type: Article

References (39)

1. Morphew MK (2007) 3D immunolocalization with plastic sections. Methods Cell Biol 79: 493-513
2. Rostaing P, Weimer RM, Jorgensen EM et al (2004) Preservation of immunoreactivity and fine structure of adult C. elegans tissues using high-pressure freezing. J Histochem Cytochem 52:1-12
3. Faulk WP, Taylor GM (1971) An immunocolloid method for the electron microscope. Immunochemistry 8:1081-1083
4. Duc-Nguyen H, Rosenblum EN (1967) Immuno-electron microscopy of the morphogenesis of mumps virus. J Virol 1:415-429
5. Feldherr CM, Marshall JM Jr (1962) The use of colloidal gold for studies of intracellular exchanges in the ameba Chaos chaos . J Cell Biol 12:640-645
6. Roth J, Bendayan M, Carlemalm E et al (1981) Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue. J Histochem Cytochem 29:663-671
7. Chalfi e M, Tu Y, Euskirchen G et al (1994) Green fluorescent protein as a marker for gene expression. Science 263:802-805
8. Shimomura O (2009) Discovery of green fluorescent protein (GFP) (Nobel Lecture). Angew Chem Int Ed Engl 48:5590-5602
9. Tsien R (1998) The green fluorescent protein. Annu Rev Biochem 67:509-544
10. Betzig E, Patterson GH, Sougrat R et al (2006) Imaging intracellular fluorescent proteins at nanometer resolution. Science 313:1642-1645
11. Hess ST, Girirajan TPK, Mason MD (2006) Ultra-high resolution imaging by fluorescence photoactivation localization microscopy. Biophys J 91:4258-4272
12. Fölling J, Bossi M, Bock H et al (2008) Fluorescence nanoscopy by ground-state depletion and single-molecule return. Nat Methods 5:943-945
13. Rust MJ, Bates M, Zhuang X (2006) Subdiffraction-limit imaging by stochastic optical reconstruction microscopy (STORM). Nat Methods 3:793-795
14. Heilemann M, Van de Linde S, Schüttpelz M et al (2008) Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes. Angew Chem Int Ed Engl 47:6172-6176
15. Hell SW, Wichmann J (1994) Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy. Opt Lett 19:780-782
16. Gustafsson MG (2000) Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy. J Microsc (Oxford) 198: 82-87
17. Galbraith CG, Galbraith JA (2011) Superresolution microscopy at a glance. J Cell Sci 124:1607-1611
18. Shtengel G, Galbraith JA, Galbraith CG et al (2009) Interferometric fluorescent superresolution microscopy resolves 3D cellular ultrastructure. Proc Natl Acad Sci U S A 106: 3125-3130
19. Hell SW (2007) Far-fi eld optical nanoscopy. Science 316:1153-1158
20. Watanabe S, Punge A, Hollopeter G et al (2011) Protein localization in electron micrographs using fluorescence nanoscopy. Nat Methods 8: 80-84
21. Watanabe S, Jorgensen EM (2012) Visualizing proteins in electron micrographs at nanometer resolution. Methods Cell Biol 111:283-306
22. Sawaguchi A, Mcdonald KL, Karvar S et al (2002) A new approach for high-pressure freezing of primary culture cells: the fine structure and stimulation-associated transformation of cultured rabbit gastric parietal cells. J Micros (Oxford) 208:158-166
23. Hess MW, Müller M, Debbage PL et al (2000) Cryopreparation provides new insight into the effects of brefeldin A on the structure of the HepG2 Golgi apparatus. J Struct Biol 130: 63-72
24. McDonald K, Schwarz H, Müller-Reichert T et al (2010) "Tips and tricks" for high-pressure freezing of model systems. Methods Cell Biol 96:671-693
25. Pyott SJ, Rosenmund C (2002) The effects of temperature on vesicular supply and release in autaptic cultures of rat and mouse hippocampal neurons. J Physiol 539:523-535
26. Dempsey GT, Vaughan JC, Chen KH et al (2011) Evaluation of fluorophores for optimal performance in localization-based superresolution imaging. Nat Methods 8:1027-1036
27. Henriques R, Lelek M, Fornasiero EF et al (2010) QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ. Nat Methods 7:339-340
28. Van de Linde S, Wolter S, Heilemann M et al (2010) The effect of photoswitching kinetics and labeling densities on super-resolution fluorescence imaging. J Biotechnol 149:260-266
29. Lakadamyali M, Babcock H, Bates M et al (2012) 3D multicolor super-resolution imaging offers improved accuracy in neuron tracing. PLoS One 7(1):e30826
30. Lee S-H, Shin JY, Lee A et al (2012) Counting single photoactivatable fluorescent molecules by photoactivated localization microscopy (PALM). Proc Natl Acad Sci U S A 109: 17436-17441
31. Burnette DT, Sengupta P, Dai Y et al (2011) Bleaching/blinking assisted localization microscopy for superresolution imaging using standard fluorescent molecules. Proc Natl Acad Sci U S A 108:21081-21086
32. Huang B, Wang W, Bates M et al (2008) Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy. Science 319:810-813
33. Leunissen JLM, Yi H (2009) Self-pressurized rapid freezing (SPRF): a novel cryofi xation method for specimen preparation in electron microscopy. J Microsc (Oxford) 235:25-35
34. Lampe A, Haucke V, Sigrist SJ et al (2012) Multi-colour direct STORM with red emitting carbocyanines. Biol Cell 104:229-237
35. York AG, Ghitani A, Vaziri A et al (2011) Confi ned activation and subdiffractive localization enables whole-cell PALM with genetically expressed probes. Nat Methods 8:327-333
36. Weibull C, Christiansson A (1986) Extraction of proteins and membrane lipids during low temperature embedding of biological material for electron microscopy. J Microsc (Oxford) 142:79-86
37. Webster P (2007) Microwave-assisted processing and embedding for transmission electron microscopy. Methods Mol Biol 369:47-65
38. McDonald K (2007) Cryopreparation methods for electron microscopy of selected model systems. Methods Cell Biol 79:23-56
39. Link S, El-Sayed MA (1999) Spectral properties and relaxation dynamics of surface plasmon electronic oscillations in gold and silver nanodots and nanorods. J Phys Chem B 103: 8410-8426