Using a genetically encoded FRET-based reporter to visualize calcineurin phosphatase activity in living cells

Methods in Molecular Biology

Volumn 1071, Issue , 2014, Pages 139-149

  Mehta, Sohum ^a   Zhang, Jin ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Biosensor; Calcineurin; Calmodulin; FRET; Live cell imaging; NFAT; Phosphatase activity

Indexed keywords

CALCINEURIN; PHOSPHOPROTEIN PHOSPHATASE;

ARTICLE; CALCIUM SIGNALING; CELL CULTURE; ENZYME ACTIVITY; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENE EXPRESSION; HUMAN; HUMAN CELL; IMMUNE RESPONSE; MAMMAL CELL; NERVE CELL PLASTICITY; PRIORITY JOURNAL; TISSUE DIFFERENTIATION;

MAMMALIA;

CELL SURVIVAL; DNA; FLUORESCENCE RESONANCE ENERGY TRANSFER; HEK293 CELLS; HELA CELLS; HUMANS; MICROSCOPY, FLUORESCENCE; MOLECULAR IMAGING; NFATC TRANSCRIPTION FACTORS; PHOSPHORIC MONOESTER HYDROLASES; PLASMIDS; TRANSFECTION;

EID: 84934443776     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-622-1_11     Document Type: Article

References (24)

1. Hilioti Z, Cunningham KW (2004) Calcineurin: roles of the Ca2+/calmodulin-dependent protein phosphatase in diverse eukaryotes. Top Curr Genet 5:73-90
2. Rusnak F, Mertz P (2000) Calcineurin: form and function. Physiol Rev 80(4):1483-1521
3. Harris CD, Ermak G, Davies KJ (2005) Multiple roles of the DSCR1 (Adapt78 or RCAN1) gene and its protein product calcipressin 1 (or RCAN1) in disease. Cell Mol Life Sci 62(21):2477-2486. doi:10.1007/s00018-005-5085-4
4. Heineke J, Molkentin JD (2006) Regulation of cardiac hypertrophy by intracellular signalling pathways. Nat Rev Mol Cell Biol 7(8): 589-600. doi:10.1038/nrm1983
5. Heit JJ (2007) Calcineurin/NFAT signaling in the beta-cell: from diabetes to new therapeutics. Bioessays 29(10):1011-1021. doi:10.1002/bies.20644
6. Xie CW (2004) Calcium-regulated signaling pathways: role in amyloid beta-induced synaptic dysfunction. Neuromolecular Med 6(1):53-64. doi:10.1385/NMM:6:1:053
7. Mehta S, Zhang J (2011) Reporting from the field: genetically encoded fluorescent reporters uncover signaling dynamics in living biological systems. Annu Rev Biochem 80: 375-401. doi:10.1146/annurev-biochem-060409-093259
8. Newman RH, Fosbrink MD, Zhang J (2011) Genetically encodable fluorescent biosensors for tracking signaling dynamics in living cells. Chem Rev 111(5):3614-3666. doi:10.1021/cr100002u
9. Fosbrink M, Aye-Han NN, Cheong R, Levchenko A, Zhang J (2010) Visualization of JNK activity dynamics with a genetically encoded fluorescent biosensor. Proc Natl Acad Sci U S A 107(12):5459-5464. doi:10.1073/pnas. 0909671107
10. Fuller BG, Lampson MA, Foley EA, Rosasco-Nitcher S, Le KV, Tobelmann P, Brautigan DL, Stukenberg PT, Kapoor TM (2008) Midzone activation of aurora B in anaphase produces an intracellular phosphorylation gradient. Nature 453(7198):1132-1136. doi:10.1038/nature06923
11. Gao X, Zhang J (2008) Spatiotemporal analysis of differential Akt regulation in plasma membrane microdomains. Mol Biol Cell 19(10):4366-4373. doi:10.1091/mbc. E08-05-0449
12. Kunkel MT, Toker A, Tsien RY, Newton AC (2007) Calcium-dependent regulation of protein kinase D revealed by a genetically encoded kinase activity reporter. J Biol Chem 282(9):6733-6742. doi:10.1074/jbc. M608086200
13. Violin JD, Zhang J, Tsien RY, Newton AC (2003) A genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C. J Cell Biol 161(5):899-909. doi:10.1083/jcb.200302125
14. Wang Y, Botvinick EL, Zhao Y, Berns MW, Usami S, Tsien RY, Chien S (2005) Visualizing the mechanical activation of Src. Nature 434(7036):1040-1045. doi:10.1038/nature 03469
15. Zhang J, Hupfeld CJ, Taylor SS, Olefsky JM, Tsien RY (2005) Insulin disrupts beta-adrenergic signalling to protein kinase A in adipocytes. Nature 437(7058):569-573. doi:10.1038/nature04140
16. Newman RH, Zhang J (2008) Visualization of phosphatase activity in living cells with a FRET-based calcineurin activity sensor. Mol Biosyst 4(6):496-501. doi:10.1039/b720034j
17. Crabtree GR, Olson EN (2002) NFAT signaling: choreographing the social lives of cells. Cell 109(Suppl):S67-S79
18. Hogan PG, Chen L, Nardone J, Rao A (2003) Transcriptional regulation by calcium, calcineurin, and NFAT. Genes Dev 17(18): 2205-2232
19. Horsley V, Pavlath GK (2002) NFAT: ubiquitous regulator of cell differentiation and adaptation. J Cell Biol 156(5):771-774
20. Okamura H, Aramburu J, Garcia-Rodriguez C, Viola JP, Raghavan A, Tahiliani M, Zhang X, Qin J, Hogan PG, Rao A (2000) Concerted dephosphorylation of the transcription factor NFAT1 induces a conformational switch that regulates transcriptional activity. Mol Cell 6(3):539-550
21. Porter CM, Havens MA, Clipstone NA (2000) Identification of amino acid residues and protein kinases involved in the regulation of NFATc subcellular localization. J Biol Chem 275(5):3543-3551
22. Ananthanarayanan B, Ni Q, Zhang J (2008) Chapter 2: molecular sensors based on fluorescence resonance energy transfer to visualize cellular dynamics. Methods Cell Biol 89:37-57: doi:10.1016/S0091-679X(08)00602-X
23. Miyawaki A, Tsien RY (2000) Monitoring protein conformations and interactions by fluorescence resonance energy transfer between mutants of green fluorescent protein. Methods Enzymol 327:472-500
24. Depry C, Zhang J (2011) Using FRETbased reporters to visualize subcellular dynamics of protein kinase A activity. Methods Mol Biol 756:285-294. doi:10.1007/978-1-61779-160-4-16