Using next-generation sequencing for discovery of high-frequency monoclonal antibodies in the variable gene repertoires from immunized mice

Methods in Molecular Biology

Volumn 1131, Issue , 2014, Pages 191-203

  Haessler, Ulrike ^a   Reddy, Sai T ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

Fab; Immune repertoire; Plasma cells; Systems immunology; Variable region CDR3

Indexed keywords

DNA; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; MONOCLONAL ANTIBODY; RECOMBINANT ANTIBODY;

ANIMAL CELL; ANIMAL EXPERIMENT; ANTIBODY ISOLATION; ANTIGEN SPECIFICITY; ARTICLE; BASE PAIRING; BIOINFORMATICS; BONE MARROW CELL; CONTROLLED STUDY; DNA PURIFICATION; DNA SEQUENCE; DNA SYNTHESIS; FEMALE; GENE EXPRESSION; GENE FREQUENCY; IMMUNOGLOBULIN HEAVY CHAIN GENE; IMMUNOGLOBULIN LIGHT CHAIN GENE; IMMUNOGLOBULIN VARIABLE REGION; MOUSE; NONHUMAN; PLASMA CELL; PRIORITY JOURNAL;

ANIMALS; ANTIBODIES, MONOCLONAL; COMPUTATIONAL BIOLOGY; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN LIGHT CHAINS; IMMUNOGLOBULIN VARIABLE REGION; MICE;

EID: 84934443698     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-992-5_12     Document Type: Article

References (13)

1. Walsh G (2010) Biopharmaceutical benchmarks 2010. Nat Biotechnol 28:917-924
2. Clackson T, Hoogenboom HR, Griffi ths AD, Winter G (1991) Making antibody fragments using phage display libraries. Nature 352: 624-628
3. Feldhaus MJ, Siegel RW, Opresko LK, Coleman JR, Feldhaus JMW, Yeung YA, Cochran JR, Heinzelman P, Colby D, Swers J, Graff C, Wiley HS, Wittrup KD (2003) Flowcytometric isolation of human antibodies from a nonimmune Saccharomyces cerevisiae surface display library. Nat Biotechnol 21:163-170
4. Harvey BR, Georgiou G, Hayhurst A, Jeong KJ, Iverson BL, Rogers GK (2004) Anchored periplasmic expression, a versatile technology for the isolation of high-affinity antibodies from Escherichia coli-expressed libraries. Proc Natl Acad Sci U S A 101:9193-9198
5. Mazor Y, Van Blarcom T, Mabry R, Iverson BL, Georgiou G (2007) Isolation of engineered, full-length antibodies from libraries expressed in Escherichia coli. Nat Biotechnol 25:563-565
6. Hoogenboom HR (2005) Selecting and screening recombinant antibody libraries. Nat Biotechnol 23:1105-1116
7. Radbruch A, Muehlinghaus G, Luger EO, Inamine A, Smith KGC, Dörner T, Hiepe F (2006) Competence and competition: the challenge of becoming a long-lived plasma cell. Nat Rev Immunol 6:10
8. Calame KL (2001) Plasma cells: fi nding new light at the end of B cell development. Nat Immunol 2:1103-1108
9. Höfer T, Muehlinghaus G, Moser K, Yoshida T, E Mei H, Hebel K, Hauser A, Hoyer B, Luger O, Dörner T, Manz RA, Manz F, Hiepe F, Radbruch A (2006) Adaptation of humoral memory. Immunol Rev 211: 295-302
10. Manz RA, Thiel A, Radbruch A (1997) Lifetime of plasma cells in the bone marrow. Nature 388:133-134
11. Manz RA, Hauser AE, Hiepe F, Radbruch A (2005) Maintenance of serum antibody levels. Annu Rev Immunol 23:367-386
12. Reddy ST, Ge X, Miklos AE, Hughes RA, Kang SH, Hoi KH, Chrysostomou C, Hunicke-Smith SP, Iverson BL, Tucker PW, Ellington AD, Georgiou G (2010) Monoclonal antibodies isolated without screening by analyzing the variable-gene repertoire of plasma cells. Nat Biotechnol 28:957-961
13. Alamyar E, Giudicelli V, Li S, Duroux P, Lefranc M-P (2012) IMGT/HighV-QUEST: the IMGT ® web portal for immunoglobulin (IG) or antibody and T cell receptor (TR) analysis from NGS high throughput and deep sequencing. Immunome Res 8:26