Engineering and flow-cytometric analysis of chimeric LAGLIDADG homing endonucleases from homologous I-OnuI-family enzymes

Methods in Molecular Biology

Volumn 1123, Issue , 2014, Pages 191-221

  Baxter, Sarah K ^a,b,c   Scharenberg, Andrew M ^a,b,c   Lambert, Abigail R ^b,c  

^a UNIVERSITY OF WASHINGTON   (United States)

^b SEATTLE CHILDREN S RESEARCH INSTITUTE   (United States)

^c Northwest Genome Engineering Consortium   (United States)

Author keywords

Assembly PCR; Chimera; Chimerization; Flow cytometry; Homing endonuclease; LAGLIDADG; Meganuclease; Protein engineering; Yeast surface display

Indexed keywords

DOUBLE STRANDED DNA; ENDONUCLEASE; FLUORESCEIN ISOTHIOCYANATE; LAGLIDADG HOMING ENDONUCLEASE; POLYACRYLAMIDE GEL; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING AFFINITY; BINDING ASSAY; CARBOXY TERMINAL SEQUENCE; DNA BINDING; DNA SEQUENCE; EXPRESSION VECTOR; FLOW CYTOMETRY; FUNGUS GROWTH; GENETIC ENGINEERING; GENOME; NONHUMAN; PRIORITY JOURNAL; PROTEIN ENGINEERING; RESTRICTION SITE; SEQUENCE ALIGNMENT;

CLONING, MOLECULAR; ENDONUCLEASES; FLOW CYTOMETRY; GENE EXPRESSION; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; SUBSTRATE SPECIFICITY;

EID: 84934441605     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-968-0_14     Document Type: Article

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