Erratum to Cancer Biology and the Nuclear Envelope (Advances in Experimental Medicine and Biology, (2014), (773), 10.1007/978-1-4899-8032-8_1);The nuclear envelope and cancer: A diagnostic perspective and historical overview

Advances in Experimental Medicine and Biology

Volumn 773, Issue , 2014, Pages 5-26

  De Las Heras, Jose I ^a   Schirmer, Eric C ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

Cytology; Diagnostics; Karyoplasmic ratio; NETs; NPC; Nuclear lamina; Nuclear size

Indexed keywords

ARTICLE; AUTOMATION; BIOCHEMISTRY; BLADDER CARCINOMA; BREAST CANCER; CANCER CELL; CANCER DIAGNOSIS; CELL NUCLEUS MEMBRANE; CELL SHAPE; CELL SIZE; COLORECTAL CANCER; COMPUTER ANALYSIS; CYTOPLASM; HUMAN; IMAGE ANALYSIS; LIVER CANCER; LUNG SQUAMOUS CELL CARCINOMA; MELANOMA; MENINGIOMA; MICROSCOPY; MOLECULAR MECHANICS; MORPHOMETRICS; MOUTH SQUAMOUS CELL CARCINOMA; NEOPLASM; OSTEOSARCOMA; OVARY CANCER; PANCREAS CANCER; PRIORITY JOURNAL; PROGNOSIS; PROSTATE ADENOCARCINOMA; SOFT TISSUE SARCOMA; SQUAMOUS CELL CARCINOMA; STOMACH CARCINOMA; THYROID CARCINOMA; UTERINE CERVIX CANCER; UTERINE CERVIX CARCINOMA;

HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; NEOPLASMS; NUCLEAR ENVELOPE;

EID: 84934441494     PISSN: 00652598     EISSN: 22148019     Source Type: Book Series    
DOI: 10.1007/978-1-4899-8032-8_12     Document Type: Erratum

References (99)

1. David AR, Zimmerman MR (2010) Cancer: an old disease, a new disease or something in between? Nat Rev Cancer 10(10):728-733. doi: 10.1038/Nrc2914
2. Jay V (2002) Antony van Leeuwenhoek. Arch Pathol Lab Med 126(6):658-659
3. Lister JJ (1830) On some properties in achromatic object-glasses applicable to the improvement of the microscope. Phil Trans R Soc Lond 120:187-200
4. Boveri T (2008) Concerning the origin of malignant tumours by Theodor Boveri. Translated and annotated by Henry Harris. J Cell Sci 121(Suppl 1):1-84. doi: 10.1242/jcs.025742
5. Müller J (1838) Über den feineren Bau und die Formen der krankhaften Geschwülste. (On the nature and structural characteristics of cancer, and of those morbid growths which may be confounded with it) (trans: West C). G. Reimer, Berlin
6. Donne A (1844) Cours de Microscopie Complementaire des Etudes Medicales. Chez J.B Bailliere, Paris
7. Bennett JH (1845) Case 2, case of hypertrophy of the spleen and liver, in which death took place from suppuration of the blood. Edinb Med Surg J 64:413-423
8. Walshe WH (1846) The nature and treatment of cancer. Taylor and Walton, London
9. Vogel J (1847) The pathological anatomy of the human body. (The pathological anatomy of the human body) (trans: Day GEC, L.M.). H. Bailliere, London
10. Bennett JH (1849) On cancerous and cancroid growths. Sutherland & Knox, Edinburgh
11. Lebert H (1851) Traité Pratique des Maladies Cancéreuses et des Affections Curables Confondues aver le Cancer. Bailliere, Paris
12. Wilson EB (1925) The karyoplasmic ratio. In: Wilson EB (ed) The cell in development and heredity, 3rd edn. The Macmillan Company, New York, pp 727-733
13. Lebert H (1845) Physiologie pathologique ou récherches clinique, experimentales et microscopiques. Bailliere, Paris
14. Beale LS (1854) The microscope and its application to clinical medicine. Highley, London
15. Beale LS (1860) Examination of sputum from a case of cancer of the pharynx and the adjacent parts. Arch Med Lond 2:44
16. Naylor B (2000) The century for cytopathology. Acta Cytol 44(5):709-725
17. Virchow R (1863) Die Krankhaften Geschwülste, vol 1. Hirschwald, Berlin
18. Virchow R (1865) Die Krankhaften Geschwülste, vol 2. Hirschwald, Berlin
19. Virchow R (1865) Die Krankhaften Geschwülste, vol 3. Hirschwald, Berlin
20. Virchow R (1858) Die Cellularpathologie in ihrer Begrundung auf physiologische und pathologische Gewebelehre. (Cellular pathology as based upon physiological and pathological histology) (trans: Chance F). Hirschwald, Berlin
21. Wissowzky A (1876) Ueber das Eosin als reagenz auf Hämoglobin und die Bildung von Blutgefässen und Blutkörperchen bei Säugetier und Hühnerembryonen. Arch Mikrosk Anat 13:479-496
22. Papanicolaou GN (1928) New cancer diagnosis. Proceedings of the 3rd race betterment conference. RBF, Battle Creek, MI
23. Papanicolaou GN (1942) A New Procedure for Staining Vaginal Smears. Science 95(2469):438-439. doi: 10.1126/science.95.2469.438
24. Papanicolaou GN, Traut HF (1941) The diagnostic value of vaginal smears in carcinoma of the uterus. Am J Obstet Gynecol 42:193-206
25. Traut HF, Papanicolaou GN (1943) Cancer of the uterus: the vaginal smear in its diagnosis. Cal West Med 59(2):121-122
26. Fu YS, Reagan JW, Benington JL (1989) Pathology of the uterine cervix, vagina, and vulva. W.B. Saunders, Philadelphia
27. Morrison LF, Hopp ES, Wu R (1949) Diagnosis of malignancy of the nasopharynx; cytological studies by the smear technic. Ann Otol Rhinol Laryngol 58(1):18-32
28. Montgomery PW, Vonhaam E (1951) A study of the exfoliative cytology in patients with carcinoma of the oral mucosa. J Dent Res 30(3):308-313. doi: 10.1177/00220345510300030201
29. Patten SF, Reagan JW, Obenauf M (1959) An analytical approach to the diagnosis of endometrial adenocarcinoma in cytologic material. Trans Intersoc Cytol Council 7:153-156
30. Reagan JW, Patten SF (1961) An analytical study of the cellular changes in carcinoma in situ, squamous cell cancer and adenocarcinoma of the uterine cervix. Clin Obstet Gynecol 4: 1047-1127
31. Wied GL, Meier P, Clark LM (1964) An electronic data processing program for cytologic screening projects for uterine carcinoma. Acta Cytol 8(6):385-397
32. Wied GL, Messina AM, Manglano JI, Meier P, Blough RR (1964) Fluorospectrophotometric analyses of endometrial and endocervical epithelial cells. Acta Cytol 8(6):408-415
33. Wied GL, Bahr GF, Oldfi eld DG, Bartels PH (1968) Computer-assisted identifi cation of cells from uterine adenocarcinoma. A clinical feasibility study with Ticas. 1. Measurements at wavelength 530 Nm. Acta Cytol 12(5):357-370
34. Bartels PH, Wied GL (1997) Automated screening for cervical cancer: Diagnostic decision procedures. Acta Cytol 41(1):6-10
35. Bartels PH, Bahr GF, Calhoun DW, Wied GL (1970) Cell recognition by neighborhood grouping techniques in Ticas. Acta Cytol 14(6):313-324
36. Abdalla FB, Markus R, Buhmeida A, Boder J, Syrjanen K, Collan Y (2012) Estrogen receptor, progesterone receptor, and nuclear size features in female breast cancer in Libya: correlation with clinical features and survival. Anticancer Res 32(8):3485-3493
37. Nandakumar V, Kelbauskas L, Hernandez KF, Lintecum KM, Senechal P, Bussey KJ, Davies PC, Johnson RH, Meldrum DR (2012) Isotropic 3D nuclear morphometry of normal, fi brocystic and malignant breast epithelial cells reveals new structural alterations. PLoS One 7(1):e29230. doi: 10.1371/journal.pone.0029230
38. Tan PH, Goh BB, Chiang G, Bay BH (2001) Correlation of nuclear morphometry with pathologic parameters in ductal carcinoma in situ of the breast. Mod Pathol 14(10):937-941. doi: 10.1038/modpathol.3880415
39. Veta M, Kornegoor R, Huisman A, Verschuur-Maes AH, Viergever MA, Pluim JP, van Diest PJ (2012) Prognostic value of automatically extracted nuclear morphometric features in whole slide images of male breast cancer. Mod Pathol 25(12):1559-1565. doi: 10.1038/modpathol.2012.126
40. Saad RS, Kanbour-Shakir A, Lu E, Modery J, Kanbour A (2006) Cytomorphologic analysis and histological correlation of high-grade squamous intraepithelial lesions in postmenopausal women. Diagn Cytopathol 34(7):467-471. doi: 10.1002/dc.20475
41. Slater DN, Rice S, Stewart R, Melling SE, Hewer EM, Smith JH (2005) Proposed Sheffi eld quantitative criteria in cervical cytology to assist the grading of squamous cell dyskaryosis, as the British Society for Clinical Cytology defi nitions require amendment. Cytopathology 16(4):179-192. doi: 10.1111/j.1365-2303.2005.00271.x
42. Giorgadze T, Kanhere R, Pang C, Ganote C, Miller LE, Tabaczka P, Brown E, Husain M (2012) Small cell carcinoma of the cervix in liquid-based Pap test: utilization of split-sample immunocytochemical and molecular analysis. Diagn Cytopathol 40(3):214-219. doi: 10.1002/dc.21542
43. Eynard HG, Soria EA, Cuestas E, Rovasio RA, Eynard AR (2009) Assessment of colorectal cancer prognosis through nuclear morphometry. J Surg Res 154(2):345-348. doi: 10.1016/j. jss.2008.06.022
44. Malhotra S, Kazlouskaya V, Andres C, Gui J, Elston D (2013) Diagnostic cellular abnormalities in neoplastic and non-neoplastic lesions of the epidermis: a morphological and statistical study. J Cutan Pathol 40(4):371-378. doi: 10.1111/cup.12090
45. Meachem MD, Burgess HJ, Davies JL, Kidney BA (2012) Utility of nuclear morphometry in the cytologic evaluation of canine cutaneous soft tissue sarcomas. J Vet Diagn Invest 24(3):525-530. doi: 10.1177/1040638712440988
46. Ikeguchi M, Oka S, Saito H, Kondo A, Tsujitani S, Maeta M, Kaibara N (1999) Computerized nuclear morphometry: a new morphologic assessment for advanced gastric adenocarcinoma. Ann Surg 229(1):55-61
47. Ladekarl M, Baek-Hansen T, Henrik-Nielsen R, Mouritzen C, Henriques U, Sorensen FB (1995) Objective malignancy grading of squamous cell carcinoma of the lung. Stereologic estimates of mean nuclear size are of prognostic value, independent of clinical stage of disease. Cancer 76(5):797-802
48. Yan J, Zhuo S, Chen G, Wu X, Zhou D, Xie S, Jiang J, Ying M, Jia F, Chen J, Zhou J (2012) Preclinical study of using multiphoton microscopy to diagnose liver cancer and differentiate benign and malignant liver lesions. J Biomed Opt 17(2):026004. doi: 10.1117/1.JBO.17.2.026004
49. Na YR, Seok SH, Kim DJ, Han JH, Kim TH, Jung H, Lee BH, Park JH (2009) Isolation and characterization of spheroid cells from human malignant melanoma cell line WM-266-4. Tumour Biol 30(5-6):300-309. doi: 10.1159/000261073
50. Mossbacher U, Knollmayer S, Binder M, Steiner A, Wolff K, Pehamberger H (1996) Increased nuclear volume in metastasizing "thick" melanomas. J Invest Dermatol 106(3):437-440
51. Madsen C, Schroder HD (1996) Stereological estimation of nuclear mean volume in invasive meningiomas. APMIS 104(2):103-107
52. Natarajan S, Mahajan S, Boaz K, George T (2010) Prediction of lymph node metastases by preoperative nuclear morphometry in oral squamous cell carcinoma: a comparative image analysis study. Indian J Cancer 47(4):406-411. doi: 10.4103/0019-509X.73580
53. de Andrea CE, Petrilli AS, Jesus-Garcia R, Bleggi-Torres LF, Alves MT (2011) Large and round tumor nuclei in osteosarcoma: good clinical outcome. Int J Clin Exp Pathol 4(2):169-174
54. Zeimet AG, Fiegl H, Goebel G, Kopp F, Allasia C, Reimer D, Steppan I, Mueller-Holzner E, Ehrlich M, Marth C (2011) DNA ploidy, nuclear size, proliferation index and DNAhypomethylation in ovarian cancer. Gynecol Oncol 121(1):24-31. doi: 10.1016/j. ygyno.2010.12.332
55. Taira T, Kawahara A, Yamaguchi T, Abe H, Ishida Y, Okabe Y, Naito Y, Yano H, Kage M (2012) Morphometric image analysis of pancreatic disease by ThinPrep liquid-based cytology. Diagn Cytopathol 40(11):970-975. doi: 10.1002/dc.21704
56. Rashid F, Ul Haque A (2011) Frequencies of different nuclear morphological features in prostate adenocarcinoma. Ann Diagn Pathol 15(6):414-421. doi: 10.1016/j.anndiagpath.2011.06.002
57. Shih SR, Chang YC, Li HY, Liau JY, Lee CY, Chen CM, Chang TC (2013) Preoperative prediction of papillary thyroid carcinoma prognosis with the assistance of computerized morphometry of cytology samples obtained by fi ne-needle aspiration: preliminary report. Head Neck 35(1):28-34. doi: 10.1002/hed.22909
58. Helander K, Hofer PA, Holmberg G (1984) Karyometric investigations on urinary bladder carcinoma, correlated to histopathological grading. Virchows Arch A Pathol Anat Histopathol 403(2):117-125
59. van Velthoven R, Petein M, Oosterlinck WJ, Zandona C, Zlotta A, Van der Meijden AP, Pasteels JL, Roels H, Schulman C, Kiss R (1995) Image cytometry determination of ploidy level, proliferative activity, and nuclear size in a series of 314 transitional bladder cell carcinomas. Hum Pathol 26(1):3-11
60. Fukuzawa S, Hashimura T, Sasaki M, Yamabe H, Yoshida O (1995) Nuclear morphometry for improved prediction of the prognosis of human bladder carcinoma. Cancer 76(10):1790-1796
61. Suresh S (2007) Biomechanics and biophysics of cancer cells. Acta Biomater 3(4):413-438. doi: 10.1016/j.actbio.2007.04.002
62. Friedl P, Wolf K, Lammerding J (2011) Nuclear mechanics during cell migration. Curr Opin Cell Biol 23(1):55-64. doi: 10.1016/j.ceb.2010.10.015
63. Wolf K, Te Lindert M, Krause M, Alexander S, Te Riet J, Willis AL, Hoffman RM, Figdor CG, Weiss SJ, Friedl P (2013) Physical limits of cell migration: control by ECM space and nuclear deformation and tuning by proteolysis and traction force. J Cell Biol 201(7):1069-1084. doi: 10.1083/jcb.201210152
64. Ozaki T, Saijo M, Murakami K, Enomoto H, Taya Y, Sakiyama S (1994) Complex formation between lamin A and the retinoblastoma gene product: identifi cation of the domain on lamin A required for its interaction. Oncogene 9(9):2649-2653
65. Osada S, Ohmori SY, Taira M (2003) XMAN1, an inner nuclear membrane protein, antagonizes BMP signaling by interacting with Smad1 in Xenopus embryos. Development 130(9):1783-1794
66. Pan D, Estevez-Salmeron LD, Stroschein SL, Zhu X, He J, Zhou S, Luo K (2005) The integral inner nuclear membrane protein MAN1 physically interacts with the R-Smad proteins to repress signaling by the transforming growth factor-{beta} superfamily of cytokines. J Biol Chem 280(16):15992-16001. doi: 10.1074/jbc.M411234200
67. Zbarsky IB, Samarina OP, Yermolayeva LP (1964) Nuclear Proteins and Their Biosynthesis in the Tumour Cells. Acta Unio Int Contra Cancrum 20:937-940
68. Aaronson RP, Blobel G (1975) Isolation of nuclear pore complexes in association with a lamina. Proc Natl Acad Sci USA 72(3):1007-1011
69. Kuzmina SN, Buldyaeva TV, Akopov SB, Zbarsky IB (1984) Protein patterns of the nuclear matrix in differently proliferating and malignant cells. Mol Cell Biochem 58(1-2):183-186
70. Zackroff RV, Goldman AE, Jones JC, Steinert PM, Goldman RD (1984) Isolation and characterization of keratin-like proteins from cultured cells with fi broblastic morphology. J Cell Biol 98(4):1231-1237
71. McKeon FD, Kirschner MW, Caput D (1986) Homologies in both primary and secondary structure between nuclear envelope and intermediate fi lament proteins. Nature 319(6053):463-468. doi: 10.1038/319463a0
72. Fisher DZ, Chaudhary N, Blobel G (1986) cDNA sequencing of nuclear lamins A and C reveals primary and secondary structural homology to intermediate fi lament proteins. Proc Natl Acad Sci USA 83(17):6450-6454
73. Stuurman N, Heins S, Aebi U (1998) Nuclear lamins: their structure, assembly, and interactions. J Struct Biol 122(1-2):42-66. doi: 10.1006/jsbi.1998.3987
74. Janmey PA, Euteneuer U, Traub P, Schliwa M (1991) Viscoelastic properties of vimentin compared with other fi lamentous biopolymer networks. J Cell Biol 113(1):155-160
75. Schirmer EC, Gerace L (2004) The stability of the nuclear lamina polymer changes with the composition of lamin subtypes according to their individual binding strengths. J Biol Chem 279(41):42811-42817. doi: 10.1074/jbc.M407705200
76. Lammerding J, Fong LG, Ji JY, Reue K, Stewart CL, Young SG, Lee RT (2006) Lamins A and C but not lamin B1 regulate nuclear mechanics. J Biol Chem 281(35):25768-25780. doi: 10.1074/jbc.M513511200
77. Suntharalingam M, Wente SR (2003) Peering through the pore: nuclear pore complex structure, assembly, and function. Dev Cell 4(6):775-789
78. Schirmer EC, Florens L, Guan T, Yates JR 3rd, Gerace L (2003) Nuclear membrane proteins with potential disease links found by subtractive proteomics. Science 301(5638):1380-1382. doi: 10.1126/science.1088176
79. Wilkie GS, Korfali N, Swanson SK, Malik P, Srsen V, Batrakou DG, Heras J, Zuleger N, Kerr AR, Florens L, Schirmer EC (2011) Several novel nuclear envelope transmembrane proteins identifi ed in skeletal muscle have cytoskeletal associations. Mol Cell Proteomics 10(1):M110 003129. doi: 10.1074/mcp.M110. 003129
80. Korfali N, Wilkie GS, Swanson SK, Srsen V, Batrakou DG, Fairley EA, Malik P, Zuleger N, Goncharevich A, de Las HJ, Kelly DA, Kerr AR, Florens L, Schirmer EC (2010) The leukocyte nuclear envelope proteome varies with cell activation and contains novel transmembrane proteins that affect genome architecture. Mol Cell Proteomics 9(12):2571-2585. doi: 10.1074/mcp.M110.002915
81. Korfali N, Wilkie GS, Swanson SK, Srsen V, de Las HJ, Batrakou DG, Malik P, Zuleger N, Kerr AR, Florens L, Schirmer EC (2012) The nuclear envelope proteome differs notably between tissues. Nucleus 3(6):552-564. doi: 10.4161/nucl.22257
82. de Las Heras JI, Batrakou DG, Schirmer EC (2013) Cancer biology and the nuclear envelope: a convoluted relationship. Semin Cancer Biol 23(2):125-137. doi: 10.1016/j.semcancer.2012.01.008
83. Chow KH, Factor RE, Ullman KS (2012) The nuclear envelope environment and its cancer connections. Nat Rev Cancer 12(3):196-209. doi: 10.1038/nrc3219
84. Malik P, Korfali N, Srsen V, Lazou V, Batrakou DG, Zuleger N, Kavanagh DM, Wilkie GS, Goldberg MW, Schirmer EC (2010) Cell-specifi c and lamin-dependent targeting of novel transmembrane proteins in the nuclear envelope. Cell Mol Life Sci 67(8):1353-1369. doi: 10.1007/s00018-010-0257-2
85. Brachner A, Reipert S, Foisner R, Gotzmann J (2005) LEM2 is a novel MAN1-related inner nuclear membrane protein associated with A-type lamins. J Cell Sci 118(Pt 24):5797-5810. doi: 10.1242/jcs.02701
86. Chen IH, Huber M, Guan T, Bubeck A, Gerace L (2006) Nuclear envelope transmembrane proteins (NETs) that are up-regulated during myogenesis. BMC Cell Biol 7:38. doi: 10.1186/1471-2121-7-38
87. Rolls MM, Stein PA, Taylor SS, Ha E, McKeon F, Rapoport TA (1999) A visual screen of a GFP-fusion library identifi es a new type of nuclear envelope membrane protein. J Cell Biol 146(1):29-44
88. Lin F, Blake DL, Callebaut I, Skerjanc IS, Holmer L, McBurney MW, Paulin-Levasseur M, Worman HJ (2000) MAN1, an inner nuclear membrane protein that shares the LEM domain with lamina-associated polypeptide 2 and emerin. J Biol Chem 275(7):4840-4847
89. Worman HJ, Yuan J, Blobel G, Georgatos SD (1988) A lamin B receptor in the nuclear envelope. Proc Natl Acad Sci USA 85(22):8531-8534
90. Senior A, Gerace L (1988) Integral membrane proteins specifi c to the inner nuclear membrane and associated with the nuclear lamina. J Cell Biol 107(6 Pt 1):2029-2036
91. Foisner R, Gerace L (1993) Integral membrane proteins of the nuclear envelope interact with lamins and chromosomes, and binding is modulated by mitotic phosphorylation. Cell 73(7): 1267-1279
92. Hodzic DM, Yeater DB, Bengtsson L, Otto H, Stahl PD (2004) Sun2 is a novel mammalian inner nuclear membrane protein. J Biol Chem 279(24):25805-25812. doi: 10.1074/jbc.M313157200
93. Apel ED, Lewis RM, Grady RM, Sanes JR (2000) Syne-1, a dystrophin-and Klarsicht-related protein associated with synaptic nuclei at the neuromuscular junction. J Biol Chem 275(41):31986-31995. doi: 10.1074/jbc.M004775200
94. Clements L, Manilal S, Love DR, Morris GE (2000) Direct interaction between emerin and lamin A. Biochem Biophys Res Commun 267(3):709-714. doi: 10.1006/bbrc.1999.2023
95. Broers JL, Machiels BM, Kuijpers HJ, Smedts F, van den Kieboom R, Raymond Y, Ramaekers FC (1997) A-and B-type lamins are differentially expressed in normal human tissues. Histochem Cell Biol 107(6):505-517
96. Inoue H, Kauffman M, Shacham S, Landesman Y, Yang J, Evans CP, Weiss RH (2013) CRM1 blockade by selective inhibitors of nuclear export attenuates kidney cancer growth. J Urol 189(6):2317-2326. doi: 10.1016/j.juro.2012.10.018
97. Tai YT, Landesman Y, Acharya C, Calle Y, Zhong MY, Cea M, Tannenbaum D, Cagnetta A, Reagan M, Munshi AA, Senapedis W, Saint-Martin JR, Kashyap T, Shacham S, Kauffman M, Gu Y, Wu L, Ghobrial I, Zhan F, Kung AL, Schey SA, Richardson P, Munshi NC, Anderson KC (2013) CRM1 inhibition induces tumor cell cytotoxicity and impairs osteoclastogenesis in multiple myeloma: molecular mechanisms and therapeutic implications. Leukemia. doi: 10.1038/leu.2013.115
98. Salas Fragomeni RA, Chung HW, Landesman Y, Senapedis W, Saint-Martin JR, Tsao H, Flaherty KT, Shacham S, Kauffman M, Cusack JC (2013) CRM1 and BRAF inhibition synergize and induce tumor regression in BRAF-mutant melanoma. Mol Cancer Ther 12(7):1171-1179. doi: 10.1158/1535-7163.MCT-12-1171
99. Walker CJ, Oaks JJ, Santhanam R, Neviani P, Harb JG, Ferenchak G, Ellis JJ, Landesman Y, Eisfeld AK, Gabrail NY, Smith CL, Caligiuri MA, Hokland P, Roy DC, Reid A, Milojkovic D, Goldman JM, Apperley J, Garzon R, Marcucci G, Shacham S, Kauffman MG, Perrotti D (2013) Preclinical and clinical effi cacy of XPO1/CRM1 inhibition by the karyopherin inhibitor KPT-330 in Ph + leukemias. Blood 122(17):3034-3044. doi: 10.1182/blood-2013-04-495374