Preparation of short RNA by in vitro transcription

Methods in Molecular Biology

Volumn 941, Issue , 2012, Pages 59-68

  Lu, Cheng ^a   Li, Pingwei ^a  

^a TEXAS A AND M UNIVERSITY   (United States)

Author keywords

Gel filtration chromatography; In vitro transcription; Protein RNA binding studies; RNA purification; Short RNA; T7 RNA polymerase

Indexed keywords

NUCLEOTIDE; OLIGOMER; TRANSFER RNA;

ARTICLE; GEL FILTRATION CHROMATOGRAPHY; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PURIFICATION; RNA SYNTHESIS; RNA TRANSCRIPTION;

EID: 84934441278     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-113-4_5     Document Type: Article

References (12)

1. Davanloo P, Rosenberg AH, Dunn JJ, Studier FW (1984) Cloning and expression of the gene for bacteriophage T7 RNA polymerase. Proc Natl Acad Sci USA 81: 2035-2039
2. Milligan JF, Uhlenbeck OC (1989) Synthesis of small RNAs using T7 RNA polymerase. Methods Enzymol 180:51-62
3. Pokrovskaya ID, Gurevich VV (1994) In vitro transcription: preparative RNA yields in analytical scale reactions. Anal Biochem 220: 420-423
4. Hornung V, Ellegast J, Kim S, Brzzka K, Jung A, Kato H, Poeck H, Akira S, Conzelmann KK, Schlee M, Endres S, Hartmann G (2006) 5-triphosphate RNA is the ligand for RIG-I. Science 314:994-997
5. Pichlmair A, Schulz O, Tan CP, Nslund TI, Liljeström P, Weber F, Reise Sousa C (2006) RIG-I-mediated antiviral responses to singlestranded RNA bearing 5-phosphates. Science 314:997-1001
6. Lu C, Xu H, Ranjith-Kumar CT, Brooks MT, Hou TY, Hu F, Herr AB, Strong RK, Kao CC, Li P (2010) The structural basis of 5triphosphate double-stranded RNA recognition by RIG-I C-terminal domain. Structure 18: 1032-1043
7. Gruegelsiepe H, Schön A, Kirsebom LA, Hartmann RK (2005) Enzymatic RNA synthesis using bacteriophage T7 RNA polymerase. In: Hartmann RK, Bindereif A, Schön A, Westhof E (eds) Handbook of RNA biochemistry. WILEY-VCH Verlag GmbH & Co. KGaA, Germany, pp 3-21
8. Beckert B, Masquida B (2011) Synthesis of RNA by in vitro transcription. Methods Mol Biol 703:29-41
9. Lu C, Ranjith-Kumar CT, Hao L, Kao CC, Li P (2011) Crystal structure of RIG-I C-terminal domain bound to blunt-ended double-strand RNA without 5triphosphate. Nucleic Acids Res 39:1565-1575
10. Li X, Lu C, Stewart M, Xu H, Strong RK, Igumenova T, Li P (2009) Structural basis of double-stranded RNA recognition by the RIG-I like receptor MDA5. Arch Biochem Biophys 488:23-33
11. Milligan JF, Groebe DR, Witherell GW, Uhlenbeck OC (1987) Oligoribo-nucleotide synthesis using T7 RNA polymerase and synthetic DNA template. Nucleic Acids Res 15:8783-8798
12. Clarke PA (1999) Labeling and purification of RNA synthesized by in vitro transcription. Methods Mol Biol 118:1-10