Fine epitope mapping based on phage display and extensive mutagenesis of the target antigen

Methods in Molecular Biology

Volumn 1131, Issue , 2014, Pages 447-476

  Rojas, Gertrudis ^a  

^a CENTER OF MOLECULAR IMMUNOLOGY   (Cuba)

Author keywords

Antigen; ELISA screening; Epitope mapping; Kunkel mutagenesis; Monoclonal antibody; Phage display; Randomization

Indexed keywords

AMINO ACID; ANTIGEN; MONOCLONAL ANTIBODY; SOLVENT;

ANTIGEN BINDING; ANTIGENIC VARIATION; ARTICLE; BACTERIOPHAGE; CLINICAL PROTOCOL; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; EPITOPE MAPPING; HIGH THROUGHPUT SCREENING; HUMAN; MUTAGENESIS; PHAGE DISPLAY; PRIORITY JOURNAL; RANDOMIZATION; SEQUENCE ANALYSIS; SURFACE PROPERTY;

EPITOPE MAPPING; PEPTIDE LIBRARY;

EID: 84934439667     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-992-5_27     Document Type: Article

References (11)

1. Boyman O, Kovar M, Rubinstein MP et al (2006) Selective stimulation of T cell subsets with antibody-cytokine immune complexes. Science 311:1924-1927
2. Rojas G, Pupo A, Leon K et al (2013) Deciphering the molecular bases of the biological effects of antibodies against Interleukin-2: a versatile platform for fi ne epitope mapping. Immunobiology 218:105-113
3. Davies DR, Cohen GH (1996) Interactions of protein antigens with antibodies. Proc Natl Acad Sci U S A 93:7-12
4. Benjamin DC, Perdue SS (1996) Site-directed mutagenesis in epitope mapping. Methods 9: 508-515
5. Chao G, Cochran JR, Wittrup KD (2004) Fine epitope mapping of anti-epidermal growth factor receptor antibodies through random mutagenesis and yeast surface display. J Mol Biol 342:539-550
6. Smith GP, Petrenko VA (1997) Phage display. Chem Rev 97:391-410
7. Böttger V, Böttger A (2009) Epitope mapping using phage display peptide libraries. Methods Mol Biol 524:181-201
8. Deroo S, Muller CP (2001) Antigenic and immunogenic phage displayed mimotopes as substitute antigens: applications and limitations. Comb Chem High Throughput Screen 4:75-110
9. Saphire EO, Montero M, Menendez A et al (2007) Structure of a high affinity "mimotope" peptide bound to HIV-1 neutralizing antibody b12 explains its inability to elicit gp120 cross-reactive antibodies. J Mol Biol 369:696-709
10. Kunkel TA (1985) Rapid and efficient sitespecific mutagenesis without phenotypic selection. Proc Natl Acad Sci U S A 82:488-492
11. Fellouse FA, Sidhu SS (2007) Making antibodies in bacteria. In: Howard GC, Kaser MR (eds) Making and using antibodies. A practical handbook. CRC Press, Boca Raton, FL, pp 157-177