A tripartite fusion system for the selection of protein variants with increased stability in vivo

Methods in Molecular Biology

Volumn 978, Issue , 2013, Pages 1-20

  Foit, Linda ^a,b   Bardwell, James C A ^a,b  

^a HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^b UNIVERSITY OF MICHIGAN   (United States)

Author keywords

Genetic selection; Mutagenesis; Protein evolution; Protein stability; Reporter protein; Sandwich fusion; Tripartite fusion

Indexed keywords

AGAROSE; AMPICILLIN; BACTERIAL DNA; BETA LACTAMASE; CHLORAMPHENICOL; DNA POLYMERASE; PLASMID DNA; PROTEIN VARIANT; RESTRICTION ENDONUCLEASE; TETRACYCLINE;

ANTIBIOTIC RESISTANCE; ARTICLE; DNA REPLICATION ORIGIN; DNA SEQUENCE; GENETIC SELECTION; IN VIVO STUDY; MINIMUM INHIBITORY CONCENTRATION; MUTAGENESIS; MUTATION RATE; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; POINT MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN PURIFICATION; PROTEIN STABILITY; ROOM TEMPERATURE; SUPERNATANT; TRIPARTITE FUSION SYSTEM;

EID: 84934438404     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-293-3_1     Document Type: Article

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