An activity-independent selection system of thermostable protein variants

Nature Methods

Volumn 4, Issue 11, 2007, Pages 919-921

  Chautarf, Héè ^a   Blas Galindo, Emilio ^b   Menguy, Thierry ^a   Grand'Moursel, Laure ^a   Cava, Felipe ^b   Berenguer, José ^b   Delcourt, Marc ^a  

^a Biométhodes SA   (France)

^b UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA INTERFERON; BETA INTERFERON; GAMMA INTERFERON; MUTANT PROTEIN; NUCLEOTIDYLTRANSFERASE;

AMINO TERMINAL SEQUENCE; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIUM COLONY; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; GENE MUTATION; GENETIC SELECTION; MUTATIONAL ANALYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN ISOLATION; PROTEIN STRUCTURE; PROTEIN VARIANT; THERMOPHILE; THERMOPHILIC BACTERIUM; THERMOSTABILITY; THERMUS THERMOPHILUS; VALIDATION PROCESS;

AMINO ACID SUBSTITUTION; ESCHERICHIA COLI; FORMATE DEHYDROGENASES; HEAT; HUMANS; INTERFERON TYPE II; INTERFERON-ALPHA; INTERFERON-BETA; KANAMYCIN; LIPASE; NUCLEOTIDYLTRANSFERASES; PEPTIDE FRAGMENTS; PROTEIN ENGINEERING; PROTEIN FOLDING; PROTEINS; RECOMBINANT PROTEINS; THERMUS THERMOPHILUS;

EID: 35748932042     PISSN: 15487091     EISSN: 15491676     Source Type: Journal    
DOI: 10.1038/nmeth1090     Document Type: Article

References (15)

1. EijsinK, V.G., Gaseidnes, S., Borchert, T.V. & van den Burg, B. Biomol. Eng. 22, 21-30 (2005).
2. Shusta, E.V., Kieke, M.C., Parke, E., Kranz, D.M. & Wittrup, K.D. J. Mol. Biol. 292, 949-956 (1999).
3. Shusta, E.V., Holler, P.D., Kieke, M.C., Kranz, D.M. & Wittrup, K.D. Nat. Biotechnol. 18, 754-759 (2000).
4. Bommarius, A.S., Broering, J.M., Chaparro-Riggers, J.F. & Polizzi, K.M. Curr. Opin. Biotechnol. 17, 606-610 (2006).
5. Matsumura, M. & Aiba, S. J. Bool. Chem. 260, 15298-15303 (1985).
6. Waldo, G.S., Standish, B.M., Berendzen, J. & Terwilliger, T.C. Nat. Biotechnol. 17, 691-695 (1999).
7. Maxwell, K. L., Mittermaier, A.K., Forman-Kay, J.D. & Davidson, A.R. Protein Sci. 8, 1908-1911 (1999).
8. Slodowski, O., Bohm, J., Schone, B. & Otto, B. Eur J. Biochem. 202, 1133-1140 (1991).
9. Waschutza, G. et al. Protein Eng. 9, 905-912 (1996).
10. Saboulard, D. et al. Biotechniques 39, 363-368 (2005).
11. Delcourt, M. & Blesa, S. Method for massive mutagenesis. European patent 1311670 B1 and US patent 7202086.B2 (2001).
12. Cabantous, S. et al. J. Struct. Funct. Genomics 6, 113-119 (2005).
13. Park, C. & Marqusee, S. Nat. Methods 2, 207-212 (2005).
14. Beeby, M. et al. PLoS Biol 3, e309 (2005).
15. Sieber, V., Pluckthun, A. & Schmid, F.X. Nat. Biotechnol. 16 955-960 (1998).